FBSB_THEVB
ID FBSB_THEVB Reviewed; 347 AA.
AC Q8DJE9;
DT 01-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 90.
DE RecName: Full=D-fructose 1,6-bisphosphatase class 2/sedoheptulose 1,7-bisphosphatase;
DE Short=FBPase class 2/SBPase;
DE EC=3.1.3.11;
DE EC=3.1.3.37;
GN OrderedLocusNames=tll1276;
OS Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1).
OC Bacteria; Cyanobacteria; Pseudanabaenales; Thermosynechococcaceae;
OC Thermosynechococcus.
OX NCBI_TaxID=197221;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NIES-2133 / IAM M-273 / BP-1;
RX PubMed=12240834; DOI=10.1093/dnares/9.4.123;
RA Nakamura Y., Kaneko T., Sato S., Ikeuchi M., Katoh H., Sasamoto S.,
RA Watanabe A., Iriguchi M., Kawashima K., Kimura T., Kishida Y., Kiyokawa C.,
RA Kohara M., Matsumoto M., Matsuno A., Nakazaki N., Shimpo S., Sugimoto M.,
RA Takeuchi C., Yamada M., Tabata S.;
RT "Complete genome structure of the thermophilic cyanobacterium
RT Thermosynechococcus elongatus BP-1.";
RL DNA Res. 9:123-130(2002).
CC -!- FUNCTION: Catalyzes the hydrolysis of fructose 1,6-bisphosphate (Fru
CC 1,6-P2) and sedoheptulose 1,7-bisphosphate (Sed 1,7-P2) to fructose 6-
CC phosphate and sedoheptulose 7-phosphate, respectively. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=beta-D-fructose 1,6-bisphosphate + H2O = beta-D-fructose 6-
CC phosphate + phosphate; Xref=Rhea:RHEA:11064, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:32966, ChEBI:CHEBI:43474, ChEBI:CHEBI:57634; EC=3.1.3.11;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-sedoheptulose 1,7-bisphosphate + H2O = D-sedoheptulose 7-
CC phosphate + phosphate; Xref=Rhea:RHEA:17461, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:57483, ChEBI:CHEBI:58335; EC=3.1.3.37;
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC -!- PATHWAY: Carbohydrate biosynthesis; Calvin cycle.
CC -!- SUBUNIT: Homotetramer. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the FBPase class 2 family. {ECO:0000305}.
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DR EMBL; BA000039; BAC08828.1; -; Genomic_DNA.
DR RefSeq; NP_682066.1; NC_004113.1.
DR RefSeq; WP_011057116.1; NC_004113.1.
DR PDB; 5A5L; X-ray; 2.34 A; A=1-347.
DR PDBsum; 5A5L; -.
DR AlphaFoldDB; Q8DJE9; -.
DR SMR; Q8DJE9; -.
DR STRING; 197221.22295000; -.
DR EnsemblBacteria; BAC08828; BAC08828; BAC08828.
DR KEGG; tel:tll1276; -.
DR PATRIC; fig|197221.4.peg.1343; -.
DR eggNOG; COG1494; Bacteria.
DR OMA; AVFYMDK; -.
DR OrthoDB; 879338at2; -.
DR BRENDA; 3.1.3.11; 7763.
DR BRENDA; 3.1.3.37; 7763.
DR UniPathway; UPA00116; -.
DR Proteomes; UP000000440; Chromosome.
DR GO; GO:0042132; F:fructose 1,6-bisphosphate 1-phosphatase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0050278; F:sedoheptulose-bisphosphatase activity; IEA:UniProtKB-EC.
DR GO; GO:0006094; P:gluconeogenesis; IEA:InterPro.
DR GO; GO:0006071; P:glycerol metabolic process; IEA:InterPro.
DR GO; GO:0019253; P:reductive pentose-phosphate cycle; IEA:UniProtKB-UniPathway.
DR CDD; cd01516; FBPase_glpX; 1.
DR InterPro; IPR004464; FBPase_class-2/SBPase.
DR PANTHER; PTHR30447; PTHR30447; 1.
DR Pfam; PF03320; FBPase_glpX; 1.
DR PIRSF; PIRSF004532; GlpX; 1.
DR TIGRFAMs; TIGR00330; glpX; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Calvin cycle; Carbohydrate metabolism; Hydrolase; Manganese;
KW Metal-binding; Reference proteome.
FT CHAIN 1..347
FT /note="D-fructose 1,6-bisphosphatase class 2/sedoheptulose
FT 1,7-bisphosphatase"
FT /id="PRO_0000342724"
FT BINDING 33
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 57
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 97
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 100..102
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 100
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 131
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 176..178
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 198..200
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 225
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT HELIX 5..21
FT /evidence="ECO:0007829|PDB:5A5L"
FT TURN 22..25
FT /evidence="ECO:0007829|PDB:5A5L"
FT HELIX 29..44
FT /evidence="ECO:0007829|PDB:5A5L"
FT STRAND 50..57
FT /evidence="ECO:0007829|PDB:5A5L"
FT TURN 60..62
FT /evidence="ECO:0007829|PDB:5A5L"
FT STRAND 64..66
FT /evidence="ECO:0007829|PDB:5A5L"
FT STRAND 71..73
FT /evidence="ECO:0007829|PDB:5A5L"
FT HELIX 74..76
FT /evidence="ECO:0007829|PDB:5A5L"
FT HELIX 80..82
FT /evidence="ECO:0007829|PDB:5A5L"
FT TURN 86..89
FT /evidence="ECO:0007829|PDB:5A5L"
FT STRAND 90..100
FT /evidence="ECO:0007829|PDB:5A5L"
FT HELIX 102..106
FT /evidence="ECO:0007829|PDB:5A5L"
FT STRAND 113..120
FT /evidence="ECO:0007829|PDB:5A5L"
FT STRAND 131..137
FT /evidence="ECO:0007829|PDB:5A5L"
FT HELIX 139..141
FT /evidence="ECO:0007829|PDB:5A5L"
FT TURN 142..144
FT /evidence="ECO:0007829|PDB:5A5L"
FT HELIX 151..162
FT /evidence="ECO:0007829|PDB:5A5L"
FT HELIX 166..168
FT /evidence="ECO:0007829|PDB:5A5L"
FT STRAND 170..174
FT /evidence="ECO:0007829|PDB:5A5L"
FT HELIX 177..179
FT /evidence="ECO:0007829|PDB:5A5L"
FT HELIX 180..189
FT /evidence="ECO:0007829|PDB:5A5L"
FT STRAND 192..198
FT /evidence="ECO:0007829|PDB:5A5L"
FT HELIX 200..206
FT /evidence="ECO:0007829|PDB:5A5L"
FT STRAND 216..222
FT /evidence="ECO:0007829|PDB:5A5L"
FT HELIX 223..236
FT /evidence="ECO:0007829|PDB:5A5L"
FT STRAND 239..245
FT /evidence="ECO:0007829|PDB:5A5L"
FT HELIX 248..251
FT /evidence="ECO:0007829|PDB:5A5L"
FT TURN 255..258
FT /evidence="ECO:0007829|PDB:5A5L"
FT HELIX 261..270
FT /evidence="ECO:0007829|PDB:5A5L"
FT STRAND 279..281
FT /evidence="ECO:0007829|PDB:5A5L"
FT HELIX 282..285
FT /evidence="ECO:0007829|PDB:5A5L"
FT STRAND 292..299
FT /evidence="ECO:0007829|PDB:5A5L"
FT STRAND 302..304
FT /evidence="ECO:0007829|PDB:5A5L"
FT STRAND 308..310
FT /evidence="ECO:0007829|PDB:5A5L"
FT STRAND 313..322
FT /evidence="ECO:0007829|PDB:5A5L"
FT TURN 323..326
FT /evidence="ECO:0007829|PDB:5A5L"
FT STRAND 327..336
FT /evidence="ECO:0007829|PDB:5A5L"
SQ SEQUENCE 347 AA; 37492 MW; 5C2FF832B0AC27B3 CRC64;
MDNVIGLEII EVVEQAAIAS ARWMGKGDKN MADQAAVDAM RNRMNQIHMR GRIVIGEGER
DEAPMLYIGE EVGICTRPDA AQYCNPEELI EIDIAVDPCE GTNLCAYGQP GSMAVLAISE
KGGLFAAPDF YMKKLAAPPA AKGKVDIRNS ATENLKILSE CLDRAIDELV VVVMKRDRHN
DLIQEIRDAG ARVQLISDGD VSAALACAFS GTNIHALMGI GAAPEGVISA AAMRALGGHF
QGQLVYDPAV VMTKEWANRT REGNLEELKK AGITDPDKVY EAEELASGET VLFAACGITP
GMLMKGVRFF KGGARTQSLV ISTQSKTARF VDTIHMFDQQ LKSLQLY
