FBT1_ARATH
ID FBT1_ARATH Reviewed; 560 AA.
AC Q9SKZ5;
DT 31-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 2.
DT 25-MAY-2022, entry version 116.
DE RecName: Full=Folate-biopterin transporter 1, chloroplastic;
DE Flags: Precursor;
GN OrderedLocusNames=At2g32040; ORFNames=F22D22.21;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, AND
RP TISSUE SPECIFICITY.
RX PubMed=16162503; DOI=10.1074/jbc.m507432200;
RA Klaus S.M., Kunji E.R., Bozzo G.G., Noiriel A., de la Garza R.D.,
RA Basset G.J., Ravanel S., Rebeille F., Gregory J.F. III, Hanson A.D.;
RT "Higher plant plastids and cyanobacteria have folate carriers related to
RT those of trypanosomatids.";
RL J. Biol. Chem. 280:38457-38463(2005).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-67, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
RN [7]
RP FUNCTION.
RX PubMed=19923217; DOI=10.1074/jbc.m109.063651;
RA Eudes A., Kunji E.R., Noiriel A., Klaus S.M., Vickers T.J., Beverley S.M.,
RA Gregory J.F. III, Hanson A.D.;
RT "Identification of transport-critical residues in a folate transporter from
RT the folate-biopterin transporter (FBT) family.";
RL J. Biol. Chem. 285:2867-2875(2010).
CC -!- FUNCTION: Mediates folate monoglutamate transport involved in
CC tetrahydrofolate biosynthesis. It also mediates transport of
CC antifolates, such as methotrexate and aminopterin.
CC {ECO:0000269|PubMed:16162503, ECO:0000269|PubMed:19923217}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane
CC {ECO:0000269|PubMed:16162503}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:16162503}. Plastid membrane {ECO:0000305}; Multi-
CC pass membrane protein {ECO:0000305}. Note=Expressed in green as well as
CC in non-green plastids.
CC -!- TISSUE SPECIFICITY: Constitutively expressed at low level.
CC {ECO:0000269|PubMed:16162503}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype.
CC {ECO:0000269|PubMed:16162503}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Folate-
CC biopterin transporter (TC 2.A.71) family. {ECO:0000305}.
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DR EMBL; AC006223; AAD15400.2; -; Genomic_DNA.
DR EMBL; CP002685; AEC08625.1; -; Genomic_DNA.
DR EMBL; AY081306; AAL91195.1; -; mRNA.
DR EMBL; BT003363; AAO29981.1; -; mRNA.
DR EMBL; AY085525; AAM62749.1; -; mRNA.
DR PIR; C84728; C84728.
DR RefSeq; NP_565734.1; NM_128764.4.
DR AlphaFoldDB; Q9SKZ5; -.
DR BioGRID; 3111; 5.
DR IntAct; Q9SKZ5; 5.
DR STRING; 3702.AT2G32040.1; -.
DR TCDB; 2.A.71.2.2; the folate-biopterin transporter (fbt) family.
DR iPTMnet; Q9SKZ5; -.
DR PaxDb; Q9SKZ5; -.
DR PRIDE; Q9SKZ5; -.
DR ProteomicsDB; 222433; -.
DR EnsemblPlants; AT2G32040.1; AT2G32040.1; AT2G32040.
DR GeneID; 817764; -.
DR Gramene; AT2G32040.1; AT2G32040.1; AT2G32040.
DR KEGG; ath:AT2G32040; -.
DR Araport; AT2G32040; -.
DR TAIR; locus:2045462; AT2G32040.
DR eggNOG; ENOG502QPYM; Eukaryota.
DR HOGENOM; CLU_018563_3_0_1; -.
DR InParanoid; Q9SKZ5; -.
DR OrthoDB; 709372at2759; -.
DR PhylomeDB; Q9SKZ5; -.
DR PRO; PR:Q9SKZ5; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9SKZ5; baseline and differential.
DR Genevisible; Q9SKZ5; AT.
DR GO; GO:0009941; C:chloroplast envelope; IDA:TAIR.
DR GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015231; F:5-formyltetrahydrofolate transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0008517; F:folic acid transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0015350; F:methotrexate transmembrane transporter activity; IDA:UniProtKB.
DR GO; GO:0051958; P:methotrexate transport; IDA:UniProtKB.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR039309; BT1.
DR InterPro; IPR004324; FBT.
DR InterPro; IPR036259; MFS_trans_sf.
DR PANTHER; PTHR31585; PTHR31585; 1.
DR Pfam; PF03092; BT1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00788; fbt; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Membrane; Phosphoprotein; Plastid; Reference proteome;
KW Transit peptide; Transmembrane; Transmembrane helix; Transport.
FT TRANSIT 1..30
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 31..560
FT /note="Folate-biopterin transporter 1, chloroplastic"
FT /id="PRO_0000420113"
FT TRANSMEM 129..149
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 162..182
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 195..215
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 219..239
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 261..281
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 288..308
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 339..359
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 390..410
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 415..435
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 450..470
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 483..503
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 521..541
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 82..110
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 67
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19376835"
SQ SEQUENCE 560 AA; 60707 MW; FE4DD6AFF6E97912 CRC64;
MASRSLFSIS ISPSQFTVLP SINNERRRLA IAARSHRLSR RKIRKRPPDR DMSSIVSIPE
LPRRRDSEES LLLDSRISVA EGDTSNTDVE GDRDTTSSIR TQPPPRKGRN LSSSRIKFYG
VELSPDNVAV AMVYFVQGVL GLARLAVSFY LKDDLHLDPA ETAVITGLSS LPWLVKPLYG
FISDSVPLFG YRRRSYLVLS GLLGAFSWSL MAGFVDSKYS AAFCILLGSL SVAFSDVVVD
SMVVERARGE SQSVSGSLQS LCWGSSAFGG IVSSYFSGSL VESYGVRFVF GVTALLPLIT
SAVAVLVNEQ RVVRPASGQK ENITLLSPGF LQTSKQNMIQ LWGAIKQPNV FLPTLFIFLW
QATPHSDSAM FYFTTNKLGF TPEFLGRVKL VTSIASLLGV GLYNGFLKTV PLRKIFLVTT
IFGTGLGMTQ VILVSGFNRQ LGISDEWFAI GDSLILTVLA QASFMPVLVL AARLCPEGME
ATLFATLMSI SNGGSVLGGL MGAGLTQAFG ITRDSFGNLS TLIILCNLSS LLPLPLLGLL
PRDSPDTLTK DDADVEMKSN
