FBT5_ARATH
ID FBT5_ARATH Reviewed; 492 AA.
AC F4KIL8; F4KIM0; Q67YF0;
DT 31-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT 31-OCT-2012, sequence version 2.
DT 25-MAY-2022, entry version 67.
DE RecName: Full=Probable folate-biopterin transporter 5;
GN OrderedLocusNames=At5g25040; ORFNames=T11H3.50;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENE FAMILY.
RX PubMed=16162503; DOI=10.1074/jbc.m507432200;
RA Klaus S.M., Kunji E.R., Bozzo G.G., Noiriel A., de la Garza R.D.,
RA Basset G.J., Ravanel S., Rebeille F., Gregory J.F. III, Hanson A.D.;
RT "Higher plant plastids and cyanobacteria have folate carriers related to
RT those of trypanosomatids.";
RL J. Biol. Chem. 280:38457-38463(2005).
CC -!- FUNCTION: Could mediate folate transport. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=F4KIL8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=F4KIL8-2; Sequence=VSP_044406, VSP_044407;
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Folate-
CC biopterin transporter (TC 2.A.71) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AED93392.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC005964; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002688; AED93392.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; ANM69429.1; -; Genomic_DNA.
DR EMBL; AK176518; BAD44281.1; -; mRNA.
DR RefSeq; NP_001190390.1; NM_001203461.1.
DR RefSeq; NP_001331109.1; NM_001343908.1. [F4KIL8-1]
DR AlphaFoldDB; F4KIL8; -.
DR SMR; F4KIL8; -.
DR STRING; 3702.AT5G25040.2; -.
DR iPTMnet; F4KIL8; -.
DR PaxDb; F4KIL8; -.
DR PRIDE; F4KIL8; -.
DR ProteomicsDB; 222580; -. [F4KIL8-1]
DR EnsemblPlants; AT5G25040.4; AT5G25040.4; AT5G25040. [F4KIL8-1]
DR GeneID; 832574; -.
DR Gramene; AT5G25040.4; AT5G25040.4; AT5G25040. [F4KIL8-1]
DR KEGG; ath:AT5G25040; -.
DR Araport; AT5G25040; -.
DR TAIR; locus:2179275; AT5G25040.
DR eggNOG; ENOG502QPUV; Eukaryota.
DR HOGENOM; CLU_018563_3_2_1; -.
DR InParanoid; F4KIL8; -.
DR OMA; KFFWYTD; -.
DR OrthoDB; 659174at2759; -.
DR PRO; PR:F4KIL8; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; F4KIL8; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009536; C:plastid; HDA:TAIR.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR039309; BT1.
DR InterPro; IPR036259; MFS_trans_sf.
DR PANTHER; PTHR31585; PTHR31585; 1.
DR Pfam; PF03092; BT1; 2.
DR SUPFAM; SSF103473; SSF103473; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..492
FT /note="Probable folate-biopterin transporter 5"
FT /id="PRO_0000420117"
FT TRANSMEM 46..66
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 92..112
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 117..137
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 142..162
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 186..206
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 210..230
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 264..285
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 298..318
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 328..348
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 361..381
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 396..416
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 433..453
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VAR_SEQ 424..434
FT /note="VTRTEFGNLWL -> EIRTRSSSRLR (in isoform 2)"
FT /evidence="ECO:0000303|Ref.3"
FT /id="VSP_044406"
FT VAR_SEQ 435..492
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.3"
FT /id="VSP_044407"
FT CONFLICT 19
FT /note="R -> G (in Ref. 3; BAD44281)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 492 AA; 55330 MW; E42B3FEDAB36939A CRC64;
MAREEQNIEN RGSVHVVERE SNLRSVVCGP VRWLKMLASK LHWSYVFAVV SNCGINQGFG
YSLGHVATDY YMKDVQKVQP SQYQALSAIT RISWIIFKPL FGILTDVLPI FGFHRRPYFI
LAGVIGVVSL LFISLQSNLH LYLALFWMTI SSAAMAIADV TIDACTTYNS NKHPSLASDM
QSLCSLSYSI GELLGFFMSG ILVHLVGSKG VFGLLTFTFA LVSVVGVLFS EPRVHGFSFK
QNFTNAMKAM WRTIKCSDVW QPSLYMFITR ALGLDIKEGL FYWFTDSKDG PLFAQETVGF
ILSIGSIGSI LGVLLYNLRL KDHPFRKLFL WTQLLFALSG MFDLILVLRL NLKFGLPDYL
FIVVDGIVSK MIIRLTWMVI FVLNTKLCPH GIEGTFFALL MSIDNAGLMT SSWLGGKMLH
VLKVTRTEFG NLWLAVLVRN VMRLLPICFL FLVPQGDQNT FKLPAEIMGE DSEEEGTRNL
ELASLVDYVD RS
