FBT8_ARATH
ID FBT8_ARATH Reviewed; 542 AA.
AC F4I5Q2; O23028; Q8GYA1;
DT 31-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 64.
DE RecName: Full=Probable folate-biopterin transporter 8, chloroplastic;
DE Flags: Precursor;
GN OrderedLocusNames=At1g04570; ORFNames=T1G11.18;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP GENE FAMILY.
RX PubMed=16162503; DOI=10.1074/jbc.m507432200;
RA Klaus S.M., Kunji E.R., Bozzo G.G., Noiriel A., de la Garza R.D.,
RA Basset G.J., Ravanel S., Rebeille F., Gregory J.F. III, Hanson A.D.;
RT "Higher plant plastids and cyanobacteria have folate carriers related to
RT those of trypanosomatids.";
RL J. Biol. Chem. 280:38457-38463(2005).
CC -!- FUNCTION: Could mediate folate transport. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane {ECO:0000305};
CC Multi-pass membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Folate-
CC biopterin transporter (TC 2.A.71) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB80629.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC002376; AAB80629.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE27716.1; -; Genomic_DNA.
DR EMBL; AK117774; BAC42421.1; -; mRNA.
DR EMBL; BT005916; AAO64851.1; -; mRNA.
DR PIR; C86178; C86178.
DR RefSeq; NP_171951.2; NM_100336.3.
DR AlphaFoldDB; F4I5Q2; -.
DR STRING; 3702.AT1G04570.1; -.
DR PaxDb; F4I5Q2; -.
DR PRIDE; F4I5Q2; -.
DR ProteomicsDB; 222584; -.
DR EnsemblPlants; AT1G04570.1; AT1G04570.1; AT1G04570.
DR GeneID; 839486; -.
DR Gramene; AT1G04570.1; AT1G04570.1; AT1G04570.
DR KEGG; ath:AT1G04570; -.
DR Araport; AT1G04570; -.
DR TAIR; locus:2197803; AT1G04570.
DR eggNOG; ENOG502QSTI; Eukaryota.
DR HOGENOM; CLU_018563_0_0_1; -.
DR InParanoid; F4I5Q2; -.
DR OMA; KLIGMVQ; -.
DR OrthoDB; 709372at2759; -.
DR PRO; PR:F4I5Q2; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; F4I5Q2; baseline and differential.
DR GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR039309; BT1.
DR InterPro; IPR004324; FBT.
DR InterPro; IPR036259; MFS_trans_sf.
DR PANTHER; PTHR31585; PTHR31585; 1.
DR Pfam; PF03092; BT1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00788; fbt; 1.
PE 2: Evidence at transcript level;
KW Chloroplast; Membrane; Plastid; Reference proteome; Transit peptide;
KW Transmembrane; Transmembrane helix; Transport.
FT TRANSIT 1..78
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 79..542
FT /note="Probable folate-biopterin transporter 8,
FT chloroplastic"
FT /id="PRO_0000420120"
FT TRANSMEM 103..123
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 132..152
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 155..175
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 181..201
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 223..243
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 246..266
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 308..328
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 338..358
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 369..389
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 404..424
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 446..466
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 477..497
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 24..45
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 506..542
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 520..542
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 235
FT /note="N -> S (in Ref. 3; BAC42421 and 4; AAO64851)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 542 AA; 59507 MW; D66B490A6AF90960 CRC64;
MERIMINPLL SIQNSPTKFL KPPLSSIHRQ QQQQERQSNN NTLFMGEARP RRATTFACLS
IRRRRNNNGV SEFEETARFE QVGGKGISVL CGLGYWVQGS RCFPWLALNF HMVHSLALQP
STLQLVQYSC SLPMVAKPLY GVLSDVLYIG SGRRVPYIAI GVFLQVLAWG SMGIFQGARE
VLPSLVACVL LSNLGASITE VAKDALVAEY GLRYRINGLQ SYALMASAAG GVLGNLLGGY
LLLTTPPKIS FLVFSALLSL QLVVSLSSKE ESFGLPRIAE TSSVLESVKK QISNLKEAIQ
ADEISQPLIW AVVSIAMVPL LSGSVFCYQT QVLNLDPSVI GMSKVIGQLM LLCLTVVYDR
YLKTLPMRPL IHIIQLLYGL SILLDYILVK QINLGFGISN EVYVLCFSSL AEILAQFKIL
PFAVRLASMC PQGCEGSVTS FLASTLCLSQ IVSAFLGVGL ANLIGITSSN YSNLSSGILI
QSLAALAPLC FMHLVPMSEP VIEKEGKRGI SKRSRRNRRV GRVVDKESVT YRRERESEEA
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