ID   FBT_SYNY3               Reviewed;         494 AA.
AC   Q55721;
DT   31-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 106.
DE   RecName: Full=Folate-biopterin transporter;
GN   OrderedLocusNames=slr0642;
OS   Synechocystis sp. (strain PCC 6803 / Kazusa).
OC   Bacteria; Cyanobacteria; Synechococcales; Merismopediaceae; Synechocystis;
OC   unclassified Synechocystis.
OX   NCBI_TaxID=1111708;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PCC 6803 / Kazusa;
RX   PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA   Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA   Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T.,
RA   Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.,
RA   Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT   "Sequence analysis of the genome of the unicellular cyanobacterium
RT   Synechocystis sp. strain PCC6803. II. Sequence determination of the entire
RT   genome and assignment of potential protein-coding regions.";
RL   DNA Res. 3:109-136(1996).
RN   [2]
RP   FUNCTION.
RX   PubMed=16162503; DOI=10.1074/jbc.m507432200;
RA   Klaus S.M., Kunji E.R., Bozzo G.G., Noiriel A., de la Garza R.D.,
RA   Basset G.J., Ravanel S., Rebeille F., Gregory J.F. III, Hanson A.D.;
RT   "Higher plant plastids and cyanobacteria have folate carriers related to
RT   those of trypanosomatids.";
RL   J. Biol. Chem. 280:38457-38463(2005).
RN   [3]
RP   MUTAGENESIS OF ARG-53; LYS-61; ASP-62; TRP-82; LYS-85; ASP-93; ASP-145;
RP   ASP-149; ARG-155; PHE-252; THR-259; GLU-263; PHE-267; ARG-309; ARG-335;
RP   ASP-348; GLN-357; ARG-369; CYS-371; PHE-380; MET-384 AND ASN-388, AND
RP   FUNCTION.
RX   PubMed=19923217; DOI=10.1074/jbc.m109.063651;
RA   Eudes A., Kunji E.R., Noiriel A., Klaus S.M., Vickers T.J., Beverley S.M.,
RA   Gregory J.F. III, Hanson A.D.;
RT   "Identification of transport-critical residues in a folate transporter from
RT   the folate-biopterin transporter (FBT) family.";
RL   J. Biol. Chem. 285:2867-2875(2010).
CC   -!- FUNCTION: Mediates folate monoglutamate transport involved in
CC       tetrahydrofolate biosynthesis. It also mediates transport of
CC       antifolates, such as methotrexate and aminopterin.
CC       {ECO:0000269|PubMed:16162503, ECO:0000269|PubMed:19923217}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily. Folate-
CC       biopterin transporter (TC 2.A.71) family. {ECO:0000305}.
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DR   EMBL; BA000022; BAA10362.1; -; Genomic_DNA.
DR   PIR; S76516; S76516.
DR   AlphaFoldDB; Q55721; -.
DR   IntAct; Q55721; 9.
DR   STRING; 1148.1001631; -.
DR   TCDB; 2.A.71.2.3; the folate-biopterin transporter (fbt) family.
DR   PaxDb; Q55721; -.
DR   PRIDE; Q55721; -.
DR   EnsemblBacteria; BAA10362; BAA10362; BAA10362.
DR   KEGG; syn:slr0642; -.
DR   eggNOG; COG2211; Bacteria.
DR   InParanoid; Q55721; -.
DR   OMA; GIDDHWF; -.
DR   PhylomeDB; Q55721; -.
DR   Proteomes; UP000001425; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015231; F:5-formyltetrahydrofolate transmembrane transporter activity; IDA:UniProtKB.
DR   GO; GO:0008517; F:folic acid transmembrane transporter activity; IDA:UniProtKB.
DR   GO; GO:0015350; F:methotrexate transmembrane transporter activity; IDA:UniProtKB.
DR   GO; GO:0015885; P:5-formyltetrahydrofolate transport; IDA:UniProtKB.
DR   GO; GO:0015884; P:folic acid transport; IDA:UniProtKB.
DR   GO; GO:0051958; P:methotrexate transport; IDA:UniProtKB.
DR   Gene3D; 1.20.1250.20; -; 1.
DR   InterPro; IPR039309; BT1.
DR   InterPro; IPR004324; FBT.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   PANTHER; PTHR31585; PTHR31585; 1.
DR   Pfam; PF03092; BT1; 2.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   TIGRFAMs; TIGR00788; fbt; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..494
FT                   /note="Folate-biopterin transporter"
FT                   /id="PRO_0000420112"
FT   TRANSMEM        31..51
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        64..84
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        104..124
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        133..153
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        170..190
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        197..217
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        246..266
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        284..303
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        310..330
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        346..366
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        375..395
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        415..435
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          441..461
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        442..457
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MUTAGEN         53
FT                   /note="R->C: Affects 5-formyltetrahydrofolate transporter
FT                   activity."
FT                   /evidence="ECO:0000269|PubMed:19923217"
FT   MUTAGEN         61
FT                   /note="K->C: Affects 5-formyltetrahydrofolate transporter
FT                   activity."
FT                   /evidence="ECO:0000269|PubMed:19923217"
FT   MUTAGEN         62
FT                   /note="D->C: Affects 5-formyltetrahydrofolate transporter
FT                   activity."
FT                   /evidence="ECO:0000269|PubMed:19923217"
FT   MUTAGEN         82
FT                   /note="W->C: Affects 5-formyltetrahydrofolate transporter
FT                   activity."
FT                   /evidence="ECO:0000269|PubMed:19923217"
FT   MUTAGEN         85
FT                   /note="K->C: Affects 5-formyltetrahydrofolate transporter
FT                   activity."
FT                   /evidence="ECO:0000269|PubMed:19923217"
FT   MUTAGEN         93
FT                   /note="D->C: Affects 5-formyltetrahydrofolate transporter
FT                   activity."
FT                   /evidence="ECO:0000269|PubMed:19923217"
FT   MUTAGEN         145
FT                   /note="D->C: Affects 5-formyltetrahydrofolate transporter
FT                   activity."
FT                   /evidence="ECO:0000269|PubMed:19923217"
FT   MUTAGEN         149
FT                   /note="D->C: Affects 5-formyltetrahydrofolate transporter
FT                   activity."
FT                   /evidence="ECO:0000269|PubMed:19923217"
FT   MUTAGEN         155
FT                   /note="R->C: Affects 5-formyltetrahydrofolate transporter
FT                   activity."
FT                   /evidence="ECO:0000269|PubMed:19923217"
FT   MUTAGEN         252
FT                   /note="F->C: Affects 5-formyltetrahydrofolate transporter
FT                   activity."
FT                   /evidence="ECO:0000269|PubMed:19923217"
FT   MUTAGEN         259
FT                   /note="T->C: Affects 5-formyltetrahydrofolate transporter
FT                   activity."
FT                   /evidence="ECO:0000269|PubMed:19923217"
FT   MUTAGEN         263
FT                   /note="E->C: Affects 5-formyltetrahydrofolate transporter
FT                   activity."
FT                   /evidence="ECO:0000269|PubMed:19923217"
FT   MUTAGEN         267
FT                   /note="F->C: Affects 5-formyltetrahydrofolate transporter
FT                   activity."
FT                   /evidence="ECO:0000269|PubMed:19923217"
FT   MUTAGEN         309
FT                   /note="R->C: Affects 5-formyltetrahydrofolate transporter
FT                   activity."
FT                   /evidence="ECO:0000269|PubMed:19923217"
FT   MUTAGEN         335
FT                   /note="R->C: Affects 5-formyltetrahydrofolate transporter
FT                   activity."
FT                   /evidence="ECO:0000269|PubMed:19923217"
FT   MUTAGEN         348
FT                   /note="D->C: Affects 5-formyltetrahydrofolate transporter
FT                   activity."
FT                   /evidence="ECO:0000269|PubMed:19923217"
FT   MUTAGEN         357
FT                   /note="Q->C: Affects 5-formyltetrahydrofolate transporter
FT                   activity."
FT                   /evidence="ECO:0000269|PubMed:19923217"
FT   MUTAGEN         369
FT                   /note="R->C: Affects 5-formyltetrahydrofolate transporter
FT                   activity."
FT                   /evidence="ECO:0000269|PubMed:19923217"
FT   MUTAGEN         371
FT                   /note="C->A: Affects 5-formyltetrahydrofolate transporter
FT                   activity."
FT                   /evidence="ECO:0000269|PubMed:19923217"
FT   MUTAGEN         380
FT                   /note="F->C: Affects 5-formyltetrahydrofolate transporter
FT                   activity."
FT                   /evidence="ECO:0000269|PubMed:19923217"
FT   MUTAGEN         384
FT                   /note="M->C: Affects 5-formyltetrahydrofolate transporter
FT                   activity."
FT                   /evidence="ECO:0000269|PubMed:19923217"
FT   MUTAGEN         388
FT                   /note="N->C: Affects 5-formyltetrahydrofolate transporter
FT                   activity."
FT                   /evidence="ECO:0000269|PubMed:19923217"
SQ   SEQUENCE   494 AA;  53586 MW;  101BAB039B77E66A CRC64;
     MLVAMSMTPI AILFSTPLKR FLREKVLLGN APSWELLAIL SIYFVQGVLG LSRLAVSFFL
     KDELGLSPAA MGALIGLGAA PWILKPVLGL MSDTVPLFGY RRRSYLWLSG LMGSAGWLLF
     AAWVSSGTQA GLVLLFTSLS VAIGDVIVDS LVVERAQRES LAQVGSLQSL TWGAAAVGGI
     ITAYASGALL EWFSTRTVFA ITAIFPLLTV GAAFLISEVS TAEEEEKPQP KAQIKLVWQA
     VRQKTILLPT LFIFFWQATP SAESAFFYFT TNELGFEPKF LGRVRLVTSV AGLIGVGLYQ
     RFLKTLPFRV IMGWSTVISS LLGLTTLILI THANRAMGID DHWFSLGDSI ILTVTGQIAF
     MPVLVLAARL CPPGIEATLF ALLMSVMNLA GVLSFEVGSL LTHWLGVTET QFDNLALLVI
     ITNLSTLLPL PFLGLLPAGD PQVKDKTEKE DNPDDPGDRL VLPPAEVFEH HTVGSLSDQN
     FLPEFFPEKS SSRP