FBX21_HUMAN
ID FBX21_HUMAN Reviewed; 628 AA.
AC O94952; B3KMF0; Q5BJG0; Q9H087;
DT 09-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT 16-DEC-2008, sequence version 2.
DT 03-AUG-2022, entry version 170.
DE RecName: Full=F-box only protein 21;
GN Name=FBXO21; Synonyms=FBX21, KIAA0875;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RX PubMed=10531035; DOI=10.1016/s0960-9822(00)80020-2;
RA Cenciarelli C., Chiaur D.S., Guardavaccaro D., Parks W., Vidal M.,
RA Pagano M.;
RT "Identification of a family of human F-box proteins.";
RL Curr. Biol. 9:1177-1179(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Brain;
RX PubMed=10048485; DOI=10.1093/dnares/5.6.355;
RA Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N.,
RA Tanaka A., Kotani H., Nomura N., Ohara O.;
RT "Prediction of the coding sequences of unidentified human genes. XII. The
RT complete sequences of 100 new cDNA clones from brain which code for large
RT proteins in vitro.";
RL DNA Res. 5:355-364(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16541075; DOI=10.1038/nature04569;
RA Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y.,
RA Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C.,
RA Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C.,
RA Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R.,
RA Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E.,
RA Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y.,
RA Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G.,
RA Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H.,
RA Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S.,
RA Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M.,
RA Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H.,
RA Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q.,
RA Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V.,
RA Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E.,
RA Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K.,
RA Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D.,
RA Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R.,
RA David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E.,
RA D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N.,
RA Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N.,
RA Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R.,
RA Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S.,
RA LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H.,
RA Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P.,
RA Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G.,
RA Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E.,
RA Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S.,
RA Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O.,
RA Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J.,
RA Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A.,
RA Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M.,
RA Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I.,
RA Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A.,
RA Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y.,
RA Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A.,
RA Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F.,
RA Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L.,
RA Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G.,
RA Gibbs R.A.;
RT "The finished DNA sequence of human chromosome 12.";
RL Nature 440:346-351(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-628 (ISOFORM 2).
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 51-628 (ISOFORM 1).
RC TISSUE=Testis;
RX PubMed=11230166; DOI=10.1101/gr.gr1547r;
RA Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S.,
RA Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J.,
RA Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W.,
RA Ottenwaelder B., Obermaier B., Tampe J., Heubner D., Wambutt R., Korn B.,
RA Klein M., Poustka A.;
RT "Towards a catalog of human genes and proteins: sequencing and analysis of
RT 500 novel complete protein coding human cDNAs.";
RL Genome Res. 11:422-435(2001).
CC -!- FUNCTION: Substrate-recognition component of the SCF (SKP1-CUL1-F-box
CC protein)-type E3 ubiquitin ligase complex. {ECO:0000250}.
CC -!- SUBUNIT: Directly interacts with SKP1 and CUL1. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=O94952-2; Sequence=Displayed;
CC Name=2;
CC IsoId=O94952-1; Sequence=VSP_035975;
CC -!- MISCELLANEOUS: [Isoform 1]: May be due to a competing acceptor splice
CC site.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB66833.2; Type=Erroneous termination; Note=Truncated C-terminus.; Evidence={ECO:0000305};
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DR EMBL; AF174601; AAF04522.1; -; mRNA.
DR EMBL; AB020682; BAA74898.1; -; mRNA.
DR EMBL; AK001699; BAG50962.1; -; mRNA.
DR EMBL; AC026364; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC026368; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471054; EAW98105.1; -; Genomic_DNA.
DR EMBL; BC091496; AAH91496.1; -; mRNA.
DR EMBL; AL136899; CAB66833.2; ALT_SEQ; mRNA.
DR CCDS; CCDS44989.1; -. [O94952-1]
DR CCDS; CCDS9184.1; -. [O94952-2]
DR RefSeq; NP_055817.1; NM_015002.2. [O94952-1]
DR RefSeq; NP_296373.1; NM_033624.2. [O94952-2]
DR AlphaFoldDB; O94952; -.
DR BioGRID; 116656; 60.
DR IntAct; O94952; 16.
DR MINT; O94952; -.
DR STRING; 9606.ENSP00000328187; -.
DR iPTMnet; O94952; -.
DR PhosphoSitePlus; O94952; -.
DR BioMuta; FBXO21; -.
DR EPD; O94952; -.
DR jPOST; O94952; -.
DR MassIVE; O94952; -.
DR MaxQB; O94952; -.
DR PaxDb; O94952; -.
DR PeptideAtlas; O94952; -.
DR PRIDE; O94952; -.
DR ProteomicsDB; 50572; -. [O94952-2]
DR ProteomicsDB; 50573; -. [O94952-1]
DR Antibodypedia; 31332; 300 antibodies from 22 providers.
DR DNASU; 23014; -.
DR Ensembl; ENST00000330622.9; ENSP00000328187.5; ENSG00000135108.16. [O94952-2]
DR Ensembl; ENST00000622495.5; ENSP00000483508.1; ENSG00000135108.16. [O94952-1]
DR GeneID; 23014; -.
DR KEGG; hsa:23014; -.
DR MANE-Select; ENST00000622495.5; ENSP00000483508.1; NM_015002.3; NP_055817.1. [O94952-1]
DR UCSC; uc001twj.4; human. [O94952-2]
DR CTD; 23014; -.
DR DisGeNET; 23014; -.
DR GeneCards; FBXO21; -.
DR HGNC; HGNC:13592; FBXO21.
DR HPA; ENSG00000135108; Tissue enhanced (fallopian).
DR MIM; 609095; gene.
DR neXtProt; NX_O94952; -.
DR OpenTargets; ENSG00000135108; -.
DR PharmGKB; PA28034; -.
DR VEuPathDB; HostDB:ENSG00000135108; -.
DR eggNOG; ENOG502QS7Z; Eukaryota.
DR GeneTree; ENSGT00390000005653; -.
DR InParanoid; O94952; -.
DR OMA; CVITGWD; -.
DR PhylomeDB; O94952; -.
DR TreeFam; TF313177; -.
DR PathwayCommons; O94952; -.
DR Reactome; R-HSA-8951664; Neddylation.
DR Reactome; R-HSA-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR SignaLink; O94952; -.
DR BioGRID-ORCS; 23014; 7 hits in 1116 CRISPR screens.
DR ChiTaRS; FBXO21; human.
DR GenomeRNAi; 23014; -.
DR Pharos; O94952; Tbio.
DR PRO; PR:O94952; -.
DR Proteomes; UP000005640; Chromosome 12.
DR RNAct; O94952; protein.
DR Bgee; ENSG00000135108; Expressed in corpus epididymis and 205 other tissues.
DR ExpressionAtlas; O94952; baseline and differential.
DR Genevisible; O94952; HS.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0000151; C:ubiquitin ligase complex; NAS:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; NAS:UniProtKB.
DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; NAS:UniProtKB.
DR Gene3D; 2.30.30.390; -; 1.
DR InterPro; IPR036047; F-box-like_dom_sf.
DR InterPro; IPR011722; Hemimethylated_DNA-bd_dom.
DR InterPro; IPR036623; Hemimethylated_DNA-bd_sf.
DR InterPro; IPR032698; SirB1_N.
DR Pfam; PF13369; Transglut_core2; 1.
DR Pfam; PF08755; YccV-like; 1.
DR SMART; SM00992; YccV-like; 1.
DR SUPFAM; SSF141255; SSF141255; 1.
DR SUPFAM; SSF81383; SSF81383; 1.
DR TIGRFAMs; TIGR02097; yccV; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Reference proteome; Ubl conjugation pathway.
FT CHAIN 1..628
FT /note="F-box only protein 21"
FT /id="PRO_0000119903"
FT DOMAIN 28..84
FT /note="F-box"
FT VAR_SEQ 442..448
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:10048485,
FT ECO:0000303|PubMed:10531035, ECO:0000303|PubMed:14702039,
FT ECO:0000303|PubMed:15489334"
FT /id="VSP_035975"
FT VARIANT 180
FT /note="N -> T (in dbSNP:rs11556202)"
FT /id="VAR_047919"
SQ SEQUENCE 628 AA; 72270 MW; 56144BB92D3CE446 CRC64;
MAAAAVDSAM EVVPALAEEA APEVAGLSCL VNLPGEVLEY ILCCGSLTAA DIGRVSSTCR
RLRELCQSSG KVWKEQFRVR WPSLMKHYSP TDYVNWLEEY KVRQKAGLEA RKIVASFSKR
FFSEHVPCNG FSDIENLEGP EIFFEDELVC ILNMEGRKAL TWKYYAKKIL YYLRQQKILN
NLKAFLQQPD DYESYLEGAV YIDQYCNPLS DISLKDIQAQ IDSIVELVCK TLRGINSRHP
SLAFKAGESS MIMEIELQSQ VLDAMNYVLY DQLKFKGNRM DYYNALNLYM HQVLIRRTGI
PISMSLLYLT IARQLGVPLE PVNFPSHFLL RWCQGAEGAT LDIFDYIYID AFGKGKQLTV
KECEYLIGQH VTAALYGVVN VKKVLQRMVG NLLSLGKREG IDQSYQLLRD SLDLYLAMYP
DQVQLLLLQA RLYFHLGIWP EKSFCLVLKV LDILQHIQTL DPGQHGAVGY LVQHTLEHIE
RKKEEVGVEV KLRSDEKHRD VCYSIGLIMK HKRYGYNCVI YGWDPTCMMG HEWIRNMNVH
SLPHGHHQPF YNVLVEDGSC RYAAQENLEY NVEPQEISHP DVGRYFSEFT GTHYIPNAEL
EIRYPEDLEF VYETVQNIYS AKKENIDE
