FBX21_MOUSE
ID FBX21_MOUSE Reviewed; 627 AA.
AC Q8VDH1; Q8BHA5; Q8CHC6;
DT 16-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=F-box only protein 21;
GN Name=Fbxo21; Synonyms=Fbx21, Kiaa0875;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Head, and Spinal cord;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Brain;
RX PubMed=12465718; DOI=10.1093/dnares/9.5.179;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Hara Y., Nagase T., Ohara O.,
RA Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: I.
RT The complete nucleotide sequences of 100 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 9:179-188(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Kidney, Liver, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Substrate-recognition component of the SCF (SKP1-CUL1-F-box
CC protein)-type E3 ubiquitin ligase complex. {ECO:0000250}.
CC -!- SUBUNIT: Directly interacts with SKP1 and CUL1. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8VDH1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8VDH1-2; Sequence=VSP_011350;
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC41454.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK048131; BAC33252.1; -; mRNA.
DR EMBL; AK048542; BAC33369.1; -; mRNA.
DR EMBL; AK049723; BAC33894.1; -; mRNA.
DR EMBL; AB093270; BAC41454.1; ALT_INIT; mRNA.
DR EMBL; BC021871; AAH21871.1; -; mRNA.
DR CCDS; CCDS19607.1; -. [Q8VDH1-1]
DR CCDS; CCDS84947.1; -. [Q8VDH1-2]
DR RefSeq; NP_001333517.1; NM_001346588.1. [Q8VDH1-2]
DR RefSeq; NP_663539.1; NM_145564.4. [Q8VDH1-1]
DR AlphaFoldDB; Q8VDH1; -.
DR BioGRID; 231154; 30.
DR IntAct; Q8VDH1; 26.
DR STRING; 10090.ENSMUSP00000035506; -.
DR iPTMnet; Q8VDH1; -.
DR PhosphoSitePlus; Q8VDH1; -.
DR EPD; Q8VDH1; -.
DR MaxQB; Q8VDH1; -.
DR PaxDb; Q8VDH1; -.
DR PeptideAtlas; Q8VDH1; -.
DR PRIDE; Q8VDH1; -.
DR ProteomicsDB; 271882; -. [Q8VDH1-1]
DR ProteomicsDB; 271883; -. [Q8VDH1-2]
DR Antibodypedia; 31332; 300 antibodies from 22 providers.
DR Ensembl; ENSMUST00000035579; ENSMUSP00000035506; ENSMUSG00000032898. [Q8VDH1-2]
DR Ensembl; ENSMUST00000202447; ENSMUSP00000143873; ENSMUSG00000032898. [Q8VDH1-1]
DR GeneID; 231670; -.
DR KEGG; mmu:231670; -.
DR UCSC; uc008zga.1; mouse. [Q8VDH1-1]
DR UCSC; uc008zgb.1; mouse. [Q8VDH1-2]
DR CTD; 23014; -.
DR MGI; MGI:1924223; Fbxo21.
DR VEuPathDB; HostDB:ENSMUSG00000032898; -.
DR eggNOG; ENOG502QS7Z; Eukaryota.
DR GeneTree; ENSGT00390000005653; -.
DR InParanoid; Q8VDH1; -.
DR OMA; CVITGWD; -.
DR OrthoDB; 251112at2759; -.
DR PhylomeDB; Q8VDH1; -.
DR TreeFam; TF313177; -.
DR Reactome; R-MMU-8951664; Neddylation.
DR Reactome; R-MMU-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR BioGRID-ORCS; 231670; 3 hits in 72 CRISPR screens.
DR ChiTaRS; Fbxo21; mouse.
DR PRO; PR:Q8VDH1; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; Q8VDH1; protein.
DR Bgee; ENSMUSG00000032898; Expressed in cortical plate and 251 other tissues.
DR ExpressionAtlas; Q8VDH1; baseline and differential.
DR Genevisible; Q8VDH1; MM.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR Gene3D; 2.30.30.390; -; 1.
DR InterPro; IPR036047; F-box-like_dom_sf.
DR InterPro; IPR001810; F-box_dom.
DR InterPro; IPR011722; Hemimethylated_DNA-bd_dom.
DR InterPro; IPR036623; Hemimethylated_DNA-bd_sf.
DR InterPro; IPR032698; SirB1_N.
DR Pfam; PF13369; Transglut_core2; 1.
DR Pfam; PF08755; YccV-like; 1.
DR SMART; SM00992; YccV-like; 1.
DR SUPFAM; SSF141255; SSF141255; 1.
DR SUPFAM; SSF81383; SSF81383; 1.
DR TIGRFAMs; TIGR02097; yccV; 1.
DR PROSITE; PS50181; FBOX; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Reference proteome; Ubl conjugation pathway.
FT CHAIN 1..627
FT /note="F-box only protein 21"
FT /id="PRO_0000119904"
FT DOMAIN 27..76
FT /note="F-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00080"
FT VAR_SEQ 443..449
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12465718,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_011350"
FT CONFLICT 396
FT /note="G -> S (in Ref. 1; BAC33252/BAC33369)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 627 AA; 72173 MW; A62939DCC7482112 CRC64;
MASVAGDSAM EVVPALAEEA AAEATGPSCL VQLPGEVLEY ILCSGSLTAL DIGRVSSTCR
RLREVCQSSG QVWKEQFRVR WPSLMKHYSP TDYVNWLEEY KVRQKAGLEA RKIVASFSKR
FFSEHVPCNG FSDIENLEGP EIFFEDELVC ILNMEGRKAL TWKYYAKKIL YYLRQQKILN
NLKAFLQQPD DYESYLEGAV YIDQYCNPLS DISFRDIQAQ IHSIVELVCK TLRGINSRHP
SLTFRAGESS MIMEIELQSQ VLDAINYVLY DQLKFKGNRM DYYNALNLYM HQVLTRRTGI
PISMSLLYLT VARQLGVPLE PVNFPSHFLL RWCQGAEGAT LDIFDYIYID AFGKGKQLTV
KECEYLIGQH VTAALYGVVN VKKVLQRMVG NLLSLGKREG IDQSYQLLRD SLDLYLAMYP
DQVQLLLLQA RLYFHLGIWP EKSFCLVLKV LDILQHIQTL DPGQHGAVGY LVQHTLEHIE
RKKEEVGVEV KLRSEEKHRD VCYSIGLVMK HKRYGYNCVI YGWDPTCMMG HEWIRNMNVH
SLPHGHHQPF YNVLVEDGSC RYAAQENLEY NVEPQEISHP DVGRYFSEFT GTHYIPNAEL
EIRYPEDLEF VYETVQNIYS AKEDTAE
