FBX25_MOUSE
ID FBX25_MOUSE Reviewed; 357 AA.
AC Q9D2Y6; Q9D8H3;
DT 09-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=F-box only protein 25;
GN Name=Fbxo25; Synonyms=Fbx25;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Cecum;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Kidney, and Small intestine;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=16278047; DOI=10.1016/j.bbagen.2005.09.018;
RA Hagens O., Minina E., Schweiger S., Ropers H.-H., Kalscheuer V.;
RT "Characterization of FBX25, encoding a novel brain-expressed F-box
RT protein.";
RL Biochim. Biophys. Acta 1760:110-118(2006).
RN [4]
RP FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, IDENTIFICATION IN A SCF
RP PROTEIN LIGASE COMPLEX, AND INTERACTION WITH SKP1.
RX PubMed=16714087; DOI=10.1016/j.bbagen.2006.03.020;
RA Maragno A.L., Baqui M.M., Gomes M.D.;
RT "FBXO25, an F-box protein homologue of atrogin-1, is not induced in
RT atrophying muscle.";
RL Biochim. Biophys. Acta 1760:966-972(2006).
RN [5]
RP FUNCTION, TISSUE SPECIFICITY, IDENTIFICATION IN A SCF PROTEIN LIGASE
RP COMPLEX, INTERACTION WITH SKP1, AND SUBCELLULAR LOCATION.
RX PubMed=18287534; DOI=10.1091/mbc.e07-08-0815;
RA Manfiolli A.O., Maragno A.L., Baqui M.M., Yokoo S., Teixeira F.R.,
RA Oliveira E.B., Gomes M.D.;
RT "FBXO25-associated nuclear domains: a novel subnuclear structure.";
RL Mol. Biol. Cell 19:1848-1861(2008).
CC -!- FUNCTION: Substrate-recognition component of the SCF (SKP1-CUL1-F-box
CC protein)-type E3 ubiquitin ligase complex. May play a role in
CC accumulation of expanded polyglutamine (polyQ) protein huntingtin
CC (HTT). {ECO:0000269|PubMed:16714087, ECO:0000269|PubMed:18287534}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Part of a SCF (SKP1-cullin-F-box) protein ligase complex
CC consisting of FBXO25, SKP1, CUL1 and RBX1. Interacts directly with SKP1
CC and CUL1. Interacts (via C-terminus) with beta-actin (via N-terminus)
CC (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:16714087,
CC ECO:0000269|PubMed:18287534}. Note=In the nucleus, associates with a
CC specific and novel subnuclear dot-like structure. Colocalized with
CC SKP1.
CC -!- TISSUE SPECIFICITY: Expressed in all tissues tested, except striated
CC muscle (at protein level). Expressed predominantly in the cerebral
CC cortex, the hippocampus and the Purkinje cell layer of the brain.
CC Intestine and kidney show also significant levels.
CC {ECO:0000269|PubMed:16278047, ECO:0000269|PubMed:16714087,
CC ECO:0000269|PubMed:18287534}.
CC -!- DEVELOPMENTAL STAGE: Expressed in neuronal tissues of 14.5 dpc mice.
CC {ECO:0000269|PubMed:16278047}.
CC -!- DOMAIN: The F-box is necessary for the interaction with SKP1.
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DR EMBL; AK008027; BAB25418.1; -; mRNA.
DR EMBL; AK018626; BAB31315.1; -; mRNA.
DR EMBL; BC014749; AAH14749.1; -; mRNA.
DR CCDS; CCDS22118.1; -.
DR RefSeq; NP_080061.1; NM_025785.2.
DR AlphaFoldDB; Q9D2Y6; -.
DR BioGRID; 211742; 2.
DR STRING; 10090.ENSMUSP00000039544; -.
DR iPTMnet; Q9D2Y6; -.
DR PhosphoSitePlus; Q9D2Y6; -.
DR MaxQB; Q9D2Y6; -.
DR PaxDb; Q9D2Y6; -.
DR PRIDE; Q9D2Y6; -.
DR ProteomicsDB; 272963; -.
DR Antibodypedia; 21946; 156 antibodies from 22 providers.
DR DNASU; 66822; -.
DR Ensembl; ENSMUST00000043520; ENSMUSP00000039544; ENSMUSG00000038365.
DR GeneID; 66822; -.
DR KEGG; mmu:66822; -.
DR UCSC; uc009kys.1; mouse.
DR CTD; 26260; -.
DR MGI; MGI:1914072; Fbxo25.
DR VEuPathDB; HostDB:ENSMUSG00000038365; -.
DR eggNOG; KOG3926; Eukaryota.
DR GeneTree; ENSGT00390000004915; -.
DR HOGENOM; CLU_065667_0_0_1; -.
DR InParanoid; Q9D2Y6; -.
DR OMA; CTSMSEA; -.
DR OrthoDB; 623008at2759; -.
DR PhylomeDB; Q9D2Y6; -.
DR TreeFam; TF313070; -.
DR UniPathway; UPA00143; -.
DR BioGRID-ORCS; 66822; 1 hit in 72 CRISPR screens.
DR PRO; PR:Q9D2Y6; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q9D2Y6; protein.
DR Bgee; ENSMUSG00000038365; Expressed in small intestine Peyer's patch and 266 other tissues.
DR ExpressionAtlas; Q9D2Y6; baseline and differential.
DR Genevisible; Q9D2Y6; MM.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0019005; C:SCF ubiquitin ligase complex; IDA:UniProtKB.
DR GO; GO:0003779; F:actin binding; IEA:UniProtKB-KW.
DR GO; GO:0016567; P:protein ubiquitination; IDA:UniProtKB.
DR InterPro; IPR036047; F-box-like_dom_sf.
DR InterPro; IPR040394; FBX25/32.
DR PANTHER; PTHR13123; PTHR13123; 1.
DR SUPFAM; SSF81383; SSF81383; 1.
PE 1: Evidence at protein level;
KW Actin-binding; Nucleus; Reference proteome; Ubl conjugation pathway.
FT CHAIN 1..357
FT /note="F-box only protein 25"
FT /id="PRO_0000119912"
FT DOMAIN 225..273
FT /note="F-box"
FT REGION 1..83
FT /note="Interaction with beta-actin"
FT /evidence="ECO:0000250"
FT CONFLICT 237
FT /note="N -> K (in Ref. 1; BAB25418)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 357 AA; 41826 MW; C262131D76036ADD CRC64;
MPFLGQDWRS PGWSWIKTED GWKRCDPCSH ELRSEDSQYT INHSIILNSG EEEIFNNECE
YAAKKRKKEH FGNDTAAHSF YREKWIYVHK ESTKERHGYC TLGEAFNRLD FSSAIQDIRR
FTYVVKLLQL IAKSQLTSLS GVAQKNYFNI LDKIVQKVLD DHQNPRLIKG LLQDLSSTLG
ILVRGVGKSV LVGNINIWIC RLETVLSWQQ QLQNLQVTKQ VNTGLTLSDL PLHMLNNILY
RFSDGWDIVT LGQVTPTLYM LSEDRRLWKR LCQYHFAEQQ FCRHLILSEK GHIEWKLMYF
TLQKYYPTKE QYGDTLHFCR HCSILFWKDS GHPCTAADPD SCFTPVSPEH FIDLFKF
