ID   FBX25_RAT               Reviewed;         357 AA.
AC   Q641X7;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2004, sequence version 1.
DT   25-MAY-2022, entry version 72.
DE   RecName: Full=F-box only protein 25;
GN   Name=Fbxo25;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Substrate-recognition component of the SCF (SKP1-CUL1-F-box
CC       protein)-type E3 ubiquitin ligase complex. May play a role in
CC       accumulation of expanded polyglutamine (polyQ) protein huntingtin (HTT)
CC       (By similarity). {ECO:0000250}.
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC   -!- SUBUNIT: Part of a SCF (SKP1-cullin-F-box) protein ligase complex
CC       consisting of FBXO25, SKP1, CUL1 and RBX1. Interacts directly with SKP1
CC       and CUL1 (By similarity). Interacts (via C-terminus) with actin (via N-
CC       terminus) (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Note=In the nucleus,
CC       associates with a specific and novel subnuclear dot-like structure.
CC   -!- DOMAIN: The F-box is necessary for the interaction with SKP1.
CC       {ECO:0000250}.
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DR   EMBL; BC082088; AAH82088.1; -; mRNA.
DR   RefSeq; NP_001014261.1; NM_001014239.1.
DR   AlphaFoldDB; Q641X7; -.
DR   iPTMnet; Q641X7; -.
DR   PhosphoSitePlus; Q641X7; -.
DR   GeneID; 364637; -.
DR   KEGG; rno:364637; -.
DR   CTD; 26260; -.
DR   RGD; 1359105; Fbxo25.
DR   InParanoid; Q641X7; -.
DR   OrthoDB; 623008at2759; -.
DR   PhylomeDB; Q641X7; -.
DR   UniPathway; UPA00143; -.
DR   PRO; PR:Q641X7; -.
DR   Proteomes; UP000002494; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0019005; C:SCF ubiquitin ligase complex; ISS:UniProtKB.
DR   GO; GO:0016567; P:protein ubiquitination; ISS:UniProtKB.
DR   InterPro; IPR036047; F-box-like_dom_sf.
DR   InterPro; IPR040394; FBX25/32.
DR   PANTHER; PTHR13123; PTHR13123; 1.
DR   SUPFAM; SSF81383; SSF81383; 1.
PE   2: Evidence at transcript level;
KW   Nucleus; Reference proteome; Ubl conjugation pathway.
FT   CHAIN           1..357
FT                   /note="F-box only protein 25"
FT                   /id="PRO_0000119913"
FT   DOMAIN          224..271
FT                   /note="F-box"
FT   REGION          1..83
FT                   /note="Interaction with beta-actin"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   357 AA;  41984 MW;  0AB159D60CFCED42 CRC64;
     MPFLGQDWRS PGWSWIKTED GWKRCDPCSH EIRSEDNQYP VNHSIILNSG EEEIFNNDCE
     YAAKKRKKQH FGNDTAAHSF YREKWIYVHK ESTKERHGYC TLGEAFNRLD FSSAIQDIRR
     FTYVVKLLQL IAKSQLTSLS GVAQKNYFNI LDKIVQKVLD DHQNPRLIKD LLQDLSSTLC
     ILVRGVGKSV LVGNINIWIC RLETVLNWQE KLQNLQMTKQ VNTGLTLSDL PLHMLNNILY
     RFSDGWDIVT LGQVTPTLYM LSEDRRLWKR LCQYHFAEKQ FCRHLILSEK GHIEWKLMYF
     TLQKYYPTKE QYGDTLHFCR HCSILFWKDS GHPCTAADPD SCFTPVSPEH FIDLFKF