FBX27_HUMAN
ID FBX27_HUMAN Reviewed; 283 AA.
AC Q8NI29; Q96C87;
DT 09-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT 16-AUG-2004, sequence version 2.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=F-box only protein 27;
DE AltName: Full=F-box/G-domain protein 5;
GN Name=FBXO27; Synonyms=FBG5, FBX27;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX PubMed=12383498; DOI=10.1016/s0378-1119(02)00867-3;
RA Ilyin G.P., Serandour A.L., Pigeon C., Rialland M., Glaise D.,
RA Guguen-Guillouzo C.;
RT "A new subfamily of structurally related human F-box proteins.";
RL Gene 296:11-20(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Pancreas, and Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP SUGAR-BINDING, FUNCTION, INTERACTION WITH CUL1 AND SKP1, IDENTIFICATION IN
RP SCF-COMPLEX, AND MUTAGENESIS OF 262-PHE-TRP-263.
RX PubMed=18203720; DOI=10.1074/jbc.m709508200;
RA Glenn K.A., Nelson R.F., Wen H.M., Mallinger A.J., Paulson H.L.;
RT "Diversity in tissue expression, substrate binding, and SCF complex
RT formation for a lectin family of ubiquitin ligases.";
RL J. Biol. Chem. 283:12717-12729(2008).
CC -!- FUNCTION: Substrate-recognition component of the SCF (SKP1-CUL1-F-box
CC protein)-type E3 ubiquitin ligase complex. Able to recognize and bind
CC denatured glycoproteins, which are modified with complex-type
CC oligosaccharides. {ECO:0000269|PubMed:18203720}.
CC -!- SUBUNIT: Part of a SCF (SKP1-cullin-F-box) protein ligase complex.
CC Interacts with SKP1 and CUL1. {ECO:0000269|PubMed:18203720}.
CC -!- INTERACTION:
CC Q8NI29; P63208: SKP1; NbExp=3; IntAct=EBI-6425694, EBI-307486;
CC -!- TISSUE SPECIFICITY: Predominantly expressed in brain, heart and kidney.
CC Expressed at lower levels in liver and lung.
CC {ECO:0000269|PubMed:12383498}.
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DR EMBL; AF436061; AAM27918.1; -; mRNA.
DR EMBL; BC014527; AAH14527.1; -; mRNA.
DR EMBL; BC030060; AAH30060.1; -; mRNA.
DR CCDS; CCDS12527.1; -.
DR RefSeq; NP_849142.1; NM_178820.4.
DR AlphaFoldDB; Q8NI29; -.
DR SMR; Q8NI29; -.
DR BioGRID; 125992; 26.
DR CORUM; Q8NI29; -.
DR IntAct; Q8NI29; 4.
DR STRING; 9606.ENSP00000292853; -.
DR iPTMnet; Q8NI29; -.
DR PhosphoSitePlus; Q8NI29; -.
DR BioMuta; FBXO27; -.
DR DMDM; 51338809; -.
DR jPOST; Q8NI29; -.
DR MassIVE; Q8NI29; -.
DR MaxQB; Q8NI29; -.
DR PaxDb; Q8NI29; -.
DR PeptideAtlas; Q8NI29; -.
DR PRIDE; Q8NI29; -.
DR ProteomicsDB; 73819; -.
DR Antibodypedia; 30249; 133 antibodies from 22 providers.
DR DNASU; 126433; -.
DR Ensembl; ENST00000292853.9; ENSP00000292853.3; ENSG00000161243.9.
DR Ensembl; ENST00000509137.6; ENSP00000437662.1; ENSG00000161243.9.
DR GeneID; 126433; -.
DR KEGG; hsa:126433; -.
DR MANE-Select; ENST00000292853.9; ENSP00000292853.3; NM_178820.5; NP_849142.1.
DR UCSC; uc002okh.5; human.
DR CTD; 126433; -.
DR DisGeNET; 126433; -.
DR GeneCards; FBXO27; -.
DR HGNC; HGNC:18753; FBXO27.
DR HPA; ENSG00000161243; Tissue enhanced (skin).
DR MIM; 609099; gene.
DR neXtProt; NX_Q8NI29; -.
DR OpenTargets; ENSG00000161243; -.
DR PharmGKB; PA38675; -.
DR VEuPathDB; HostDB:ENSG00000161243; -.
DR eggNOG; ENOG502RZA6; Eukaryota.
DR GeneTree; ENSGT00940000161841; -.
DR InParanoid; Q8NI29; -.
DR OMA; NNVCLHV; -.
DR OrthoDB; 922544at2759; -.
DR PhylomeDB; Q8NI29; -.
DR TreeFam; TF320527; -.
DR PathwayCommons; Q8NI29; -.
DR Reactome; R-HSA-8951664; Neddylation.
DR Reactome; R-HSA-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR SignaLink; Q8NI29; -.
DR BioGRID-ORCS; 126433; 10 hits in 1119 CRISPR screens.
DR ChiTaRS; FBXO27; human.
DR GenomeRNAi; 126433; -.
DR Pharos; Q8NI29; Tdark.
DR PRO; PR:Q8NI29; -.
DR Proteomes; UP000005640; Chromosome 19.
DR RNAct; Q8NI29; protein.
DR Bgee; ENSG00000161243; Expressed in secondary oocyte and 100 other tissues.
DR ExpressionAtlas; Q8NI29; baseline and differential.
DR Genevisible; Q8NI29; HS.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0019005; C:SCF ubiquitin ligase complex; IDA:UniProtKB.
DR GO; GO:0006516; P:glycoprotein catabolic process; IBA:GO_Central.
DR GO; GO:0016567; P:protein ubiquitination; IEA:GOC.
DR GO; GO:0031146; P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR GO; GO:0030433; P:ubiquitin-dependent ERAD pathway; IBA:GO_Central.
DR InterPro; IPR007397; F-box-assoc_dom.
DR InterPro; IPR036047; F-box-like_dom_sf.
DR InterPro; IPR001810; F-box_dom.
DR InterPro; IPR039752; F-box_only.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR PANTHER; PTHR12125; PTHR12125; 1.
DR Pfam; PF00646; F-box; 1.
DR Pfam; PF04300; FBA; 1.
DR SMART; SM01198; FBA; 1.
DR SMART; SM00256; FBOX; 1.
DR SUPFAM; SSF49785; SSF49785; 1.
DR SUPFAM; SSF81383; SSF81383; 1.
DR PROSITE; PS51114; FBA; 1.
DR PROSITE; PS50181; FBOX; 1.
PE 1: Evidence at protein level;
KW Reference proteome; Ubl conjugation pathway.
FT CHAIN 1..283
FT /note="F-box only protein 27"
FT /id="PRO_0000119914"
FT DOMAIN 23..70
FT /note="F-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00080"
FT DOMAIN 104..280
FT /note="FBA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00482"
FT REGION 1..23
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 262..263
FT /note="FW->AA: Reduces interaction with glycosylated
FT concanavalin-A in vitro."
FT /evidence="ECO:0000269|PubMed:18203720"
FT CONFLICT 8
FT /note="G -> C (in Ref. 1; AAM27918)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 283 AA; 31623 MW; 0A66E26971320B5F CRC64;
MGASVSRGRA ARVPAPEPEP EEALDLSQLP PELLLVVLSH VPPRTLLGRC RQVCRGWRAL
VDGQALWLLI LARDHGATGR ALLHLARSCQ SPARNARPCP LGRFCARRPI GRNLIRNPCG
QEGLRKWMVQ HGGDGWVVEE NRTTVPGAPS QTCFVTSFSW CCKKQVLDLE EEGLWPELLD
SGRIEICVSD WWGARHDSGC MYRLLVQLLD ANQTVLDKFS AVPDPIPQWN NNACLHVTHV
FSNIKMGVRF VSFEHRGQDT QFWAGHYGAR VTNSSVIVRV RLS
