FBX28_MOUSE
ID FBX28_MOUSE Reviewed; 368 AA.
AC Q8BIG4; Q3TS97; Q7TMH9; Q8BIF6;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=F-box only protein 28;
GN Name=Fbxo28; Synonyms=D1Ertd578e, Kiaa0483;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=12693553; DOI=10.1093/dnares/10.1.35;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S.,
RA Nakajima D., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: II.
RT The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 10:35-48(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and FVB/N; TISSUE=Brain, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-344, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Lung;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Probably recognizes and binds to some phosphorylated proteins
CC and promotes their ubiquitination and degradation. {ECO:0000250}.
CC -!- SUBUNIT: Part of a SCF (SKP1-cullin-F-box) protein ligase complex.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Chromosome, centromere, kinetochore
CC {ECO:0000250|UniProtKB:Q9NVF7}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH55458.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AK122295; BAC65577.1; -; mRNA.
DR EMBL; AK076211; BAC36256.1; -; mRNA.
DR EMBL; AK077653; BAC36929.1; -; mRNA.
DR EMBL; AK162188; BAE36778.1; -; mRNA.
DR EMBL; BC055458; AAH55458.1; ALT_INIT; mRNA.
DR EMBL; BC094542; AAH94542.1; -; mRNA.
DR CCDS; CCDS15588.1; -.
DR RefSeq; NP_780336.1; NM_175127.2.
DR AlphaFoldDB; Q8BIG4; -.
DR SMR; Q8BIG4; -.
DR BioGRID; 212559; 2.
DR STRING; 10090.ENSMUSP00000054718; -.
DR iPTMnet; Q8BIG4; -.
DR PhosphoSitePlus; Q8BIG4; -.
DR EPD; Q8BIG4; -.
DR MaxQB; Q8BIG4; -.
DR PaxDb; Q8BIG4; -.
DR PeptideAtlas; Q8BIG4; -.
DR PRIDE; Q8BIG4; -.
DR ProteomicsDB; 267580; -.
DR Antibodypedia; 34636; 192 antibodies from 26 providers.
DR DNASU; 67948; -.
DR Ensembl; ENSMUST00000051431; ENSMUSP00000054718; ENSMUSG00000047539.
DR GeneID; 67948; -.
DR KEGG; mmu:67948; -.
DR UCSC; uc007dyb.1; mouse.
DR CTD; 23219; -.
DR MGI; MGI:1261890; Fbxo28.
DR VEuPathDB; HostDB:ENSMUSG00000047539; -.
DR eggNOG; ENOG502QT70; Eukaryota.
DR GeneTree; ENSGT00390000002970; -.
DR HOGENOM; CLU_024146_1_1_1; -.
DR InParanoid; Q8BIG4; -.
DR OMA; DCLRKSK; -.
DR OrthoDB; 798303at2759; -.
DR PhylomeDB; Q8BIG4; -.
DR TreeFam; TF324672; -.
DR BioGRID-ORCS; 67948; 8 hits in 74 CRISPR screens.
DR ChiTaRS; Fbxo28; mouse.
DR PRO; PR:Q8BIG4; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; Q8BIG4; protein.
DR Bgee; ENSMUSG00000047539; Expressed in animal zygote and 251 other tissues.
DR ExpressionAtlas; Q8BIG4; baseline and differential.
DR Genevisible; Q8BIG4; MM.
DR GO; GO:0000776; C:kinetochore; ISS:UniProtKB.
DR GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR GO; GO:0000209; P:protein polyubiquitination; IBA:GO_Central.
DR InterPro; IPR036047; F-box-like_dom_sf.
DR InterPro; IPR001810; F-box_dom.
DR InterPro; IPR039719; FBXO28.
DR PANTHER; PTHR13252; PTHR13252; 1.
DR Pfam; PF00646; F-box; 1.
DR SUPFAM; SSF81383; SSF81383; 1.
DR PROSITE; PS50181; FBOX; 1.
PE 1: Evidence at protein level;
KW Centromere; Chromosome; Kinetochore; Phosphoprotein; Reference proteome;
KW Ubl conjugation pathway.
FT CHAIN 1..368
FT /note="F-box only protein 28"
FT /id="PRO_0000307725"
FT DOMAIN 61..109
FT /note="F-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00080"
FT REGION 1..56
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 328..368
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 34..49
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 342..360
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 235
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NVF7"
FT MOD_RES 242
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NVF7"
FT MOD_RES 270
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q9NVF7"
FT MOD_RES 344
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT CONFLICT 356
FT /note="D -> N (in Ref. 2; BAC36929)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 368 AA; 40978 MW; 4EA50210B5AFF175 CRC64;
MAAASEERMA EEGGGGHGDG GSCSAAGSAQ RQPPAPPSQA PPPGSQAPAA PALAPDHLPQ
NNTLVALPIV AIENILSFMS YDEISQLRLV CKRMDLVCQR MLNQGFLKVE RFHNLCQKQV
KAQLPRRESE RRNHSLARHA DILAAVETRL SLLNMTFMKY VDSNLCCFIP GKVIDEIYRV
LRYVNSTRAP QRAHEVLQEL RDISSMAMEY FDEKIVPILK RKLPGSDVSG RLMGSPPVPG
PSAALTTMQL FSKQNPSRQE VTKLQQQVRT NGAGVTVLRR EISELRTKVQ EQQKQLQDQD
QKLLEQTQII GEQNARLAEL ERKLREVMES AVGTSSGSGQ SEESPRKRRK ATEAIDSLRK
SKRLRNRK
