FBX32_MOUSE
ID FBX32_MOUSE Reviewed; 355 AA.
AC Q9CPU7;
DT 16-APR-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=F-box only protein 32;
DE AltName: Full=Atrogin-1;
DE AltName: Full=Muscle atrophy F-box protein;
DE Short=MAFbx;
GN Name=Fbxo32;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=C57BL/6J;
RX PubMed=11717410; DOI=10.1073/pnas.251541198;
RA Gomes M.D., Lecker S.H., Jagoe R.T., Navon A., Goldberg A.L.;
RT "Atrogin-1, a muscle-specific F-box protein highly expressed during muscle
RT atrophy.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:14440-14445(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Head, and Skin;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Substrate recognition component of a SCF (SKP1-CUL1-F-box
CC protein) E3 ubiquitin-protein ligase complex which mediates the
CC ubiquitination and subsequent proteasomal degradation of target
CC proteins. Probably recognizes and binds to phosphorylated target
CC proteins during skeletal muscle atrophy. Recognizes TERF1 (By
CC similarity). {ECO:0000250}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Part of the SCF (SKP1-CUL1-F-box) E3 ubiquitin-protein ligase
CC complex SCF(FBXO32) formed of CUL1, SKP1, RBX1 and FBXO32.
CC {ECO:0000250|UniProtKB:Q969P5}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q969P5}. Nucleus
CC {ECO:0000250|UniProtKB:Q969P5}. Note=Shuttles between cytoplasm and the
CC nucleus. {ECO:0000250|UniProtKB:Q969P5}.
CC -!- TISSUE SPECIFICITY: Specifically expressed in cardiac and skeletal
CC muscle.
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DR EMBL; AF441120; AAL49563.1; -; mRNA.
DR EMBL; AK014793; BAB29555.1; -; mRNA.
DR EMBL; AK014612; BAB29463.1; -; mRNA.
DR EMBL; BC027211; AAH27211.1; -; mRNA.
DR CCDS; CCDS27491.1; -.
DR RefSeq; NP_080622.1; NM_026346.3.
DR AlphaFoldDB; Q9CPU7; -.
DR BioGRID; 212401; 157.
DR CORUM; Q9CPU7; -.
DR IntAct; Q9CPU7; 2.
DR MINT; Q9CPU7; -.
DR STRING; 10090.ENSMUSP00000022986; -.
DR PhosphoSitePlus; Q9CPU7; -.
DR PaxDb; Q9CPU7; -.
DR PeptideAtlas; Q9CPU7; -.
DR PRIDE; Q9CPU7; -.
DR ProteomicsDB; 272965; -.
DR Antibodypedia; 27004; 340 antibodies from 37 providers.
DR DNASU; 67731; -.
DR Ensembl; ENSMUST00000022986; ENSMUSP00000022986; ENSMUSG00000022358.
DR GeneID; 67731; -.
DR KEGG; mmu:67731; -.
DR UCSC; uc007vtk.3; mouse.
DR CTD; 114907; -.
DR MGI; MGI:1914981; Fbxo32.
DR VEuPathDB; HostDB:ENSMUSG00000022358; -.
DR eggNOG; KOG3926; Eukaryota.
DR GeneTree; ENSGT00390000004915; -.
DR HOGENOM; CLU_065667_0_0_1; -.
DR InParanoid; Q9CPU7; -.
DR OMA; QNWVKTE; -.
DR OrthoDB; 623008at2759; -.
DR PhylomeDB; Q9CPU7; -.
DR TreeFam; TF313070; -.
DR Reactome; R-MMU-8951664; Neddylation.
DR Reactome; R-MMU-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR UniPathway; UPA00143; -.
DR BioGRID-ORCS; 67731; 1 hit in 75 CRISPR screens.
DR ChiTaRS; Fbxo32; mouse.
DR PRO; PR:Q9CPU7; -.
DR Proteomes; UP000000589; Chromosome 15.
DR RNAct; Q9CPU7; protein.
DR Bgee; ENSMUSG00000022358; Expressed in hindlimb stylopod muscle and 70 other tissues.
DR Genevisible; Q9CPU7; MM.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0019005; C:SCF ubiquitin ligase complex; ISO:MGI.
DR GO; GO:0030018; C:Z disc; IDA:MGI.
DR GO; GO:0071549; P:cellular response to dexamethasone stimulus; IDA:MGI.
DR GO; GO:0014889; P:muscle atrophy; ISO:MGI.
DR GO; GO:0010614; P:negative regulation of cardiac muscle hypertrophy; ISO:MGI.
DR GO; GO:0010666; P:positive regulation of cardiac muscle cell apoptotic process; ISO:MGI.
DR GO; GO:0016567; P:protein ubiquitination; ISS:UniProtKB.
DR GO; GO:0014894; P:response to denervation involved in regulation of muscle adaptation; IDA:UniProtKB.
DR GO; GO:0014878; P:response to electrical stimulus involved in regulation of muscle adaptation; ISO:MGI.
DR InterPro; IPR036047; F-box-like_dom_sf.
DR InterPro; IPR040394; FBX25/32.
DR PANTHER; PTHR13123; PTHR13123; 1.
DR SUPFAM; SSF81383; SSF81383; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Nucleus; Reference proteome; Ubl conjugation pathway.
FT CHAIN 1..355
FT /note="F-box only protein 32"
FT /id="PRO_0000119923"
FT DOMAIN 223..271
FT /note="F-box"
FT MOTIF 62..67
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000250"
FT MOTIF 169..173
FT /note="Nuclear export signal"
FT /evidence="ECO:0000250"
FT MOTIF 280..295
FT /note="Bipartite nuclear localization signal"
FT /evidence="ECO:0000250"
SQ SEQUENCE 355 AA; 41504 MW; F464997EB97909A5 CRC64;
MPFLGQDWRS PGQSWVKTAD GWKRFLDEKS GSFVSDLSSY CNKEVYSKEN LFSSLNYDVA
AKKRKKDIQN SKTKTQYFHQ EKWIYVHKGS TKERHGYCTL GEAFNRLDFS TAILDSRRFN
YVVRLLELIA KSQLTSLSGI AQKNFMNILE KVVLKVLEDQ QNIRLIRELL QTLYTSLCTL
VQRVGKSVLV GNINMWVYRM ETILHWQQQL NSIQISRPAF KGLTITDLPV CLQLNIMQRL
SDGRDLVSLG QAAPDLHVLS EDRLLWKRLC QYHFSERQIR KRLILSDKGQ LDWKKMYFKL
VRCYPRREQY GVTLQLCKHC HILSWKGTDH PCTANNPESC SVSLSPQDFI NLFKF
