FBX42_HUMAN
ID FBX42_HUMAN Reviewed; 717 AA.
AC Q6P3S6; B3KP30; Q5TEU8; Q86XI0; Q8N3N4; Q8N5F8; Q9BRM0; Q9P2L4;
DT 31-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=F-box only protein 42;
DE AltName: Full=Just one F-box and Kelch domain-containing protein;
GN Name=FBXO42; Synonyms=FBX42, JFK, KIAA1332;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT ALA-471.
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16710414; DOI=10.1038/nature04727;
RA Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A.,
RA Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C.,
RA Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.,
RA Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C.,
RA Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W.,
RA Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J.,
RA Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J.,
RA Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y.,
RA Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J.,
RA Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S.,
RA Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K.,
RA Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R.,
RA Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M.,
RA Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S.,
RA Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J.,
RA Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W.,
RA McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S.,
RA Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M.,
RA White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H.,
RA Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E.,
RA Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G.,
RA Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
RT "The DNA sequence and biological annotation of human chromosome 1.";
RL Nature 441:315-321(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT ALA-471.
RC TISSUE=Brain, PNS, and Uterus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 67-717.
RC TISSUE=Brain;
RX PubMed=10718198; DOI=10.1093/dnares/7.1.65;
RA Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O.;
RT "Prediction of the coding sequences of unidentified human genes. XVI. The
RT complete sequences of 150 new cDNA clones from brain which code for large
RT proteins in vitro.";
RL DNA Res. 7:65-73(2000).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 352-717, AND VARIANT ALA-471.
RC TISSUE=Melanoma;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-365; SER-373 AND THR-378, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [7]
RP FUNCTION, IDENTIFICATION IN THE SCF COMPLEX, AND INTERACTION WITH TP53.
RX PubMed=19509332; DOI=10.1073/pnas.0901864106;
RA Sun L., Shi L., Li W., Yu W., Liang J., Zhang H., Yang X., Wang Y., Li R.,
RA Yao X., Yi X., Shang Y.;
RT "JFK, a Kelch domain-containing F-box protein, links the SCF complex to p53
RT regulation.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:10195-10200(2009).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-373, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Leukemic T-cell;
RX PubMed=19690332; DOI=10.1126/scisignal.2000007;
RA Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,
RA Rodionov V., Han D.K.;
RT "Quantitative phosphoproteomic analysis of T cell receptor signaling
RT reveals system-wide modulation of protein-protein interactions.";
RL Sci. Signal. 2:RA46-RA46(2009).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-552, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=20068231; DOI=10.1126/scisignal.2000475;
RA Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L.,
RA Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.;
RT "Quantitative phosphoproteomics reveals widespread full phosphorylation
RT site occupancy during mitosis.";
RL Sci. Signal. 3:RA3-RA3(2010).
RN [10]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-365 AND SER-373, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
CC -!- FUNCTION: Substrate-recognition component of some SCF (SKP1-CUL1-F-box
CC protein)-type E3 ubiquitin ligase complex. Specifically recognizes
CC p53/TP53, promoting its ubiquitination and degradation.
CC {ECO:0000269|PubMed:19509332}.
CC -!- SUBUNIT: Component of some SCF complex, composed of CUL1, SKP1, RBX1
CC and FBXO42. Interacts (via the kelch domain) with p53/TP53; interaction
CC is direct. {ECO:0000269|PubMed:19509332}.
CC -!- INTERACTION:
CC Q6P3S6; P50570-2: DNM2; NbExp=3; IntAct=EBI-2506081, EBI-10968534;
CC Q6P3S6; P49821: NDUFV1; NbExp=3; IntAct=EBI-2506081, EBI-748312;
CC Q6P3S6; Q06330: RBPJ; NbExp=3; IntAct=EBI-2506081, EBI-632552;
CC Q6P3S6; O14773: TPP1; NbExp=3; IntAct=EBI-2506081, EBI-2800203;
CC Q6P3S6; O76024: WFS1; NbExp=3; IntAct=EBI-2506081, EBI-720609;
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DR EMBL; AK055598; BAG51542.1; -; mRNA.
DR EMBL; AL109627; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL358794; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC006174; AAH06174.1; -; mRNA.
DR EMBL; BC032439; AAH32439.1; -; mRNA.
DR EMBL; BC043410; AAH43410.1; -; mRNA.
DR EMBL; BC063864; AAH63864.1; -; mRNA.
DR EMBL; AB037753; BAA92570.1; -; mRNA.
DR EMBL; AL833874; CAD38731.1; -; mRNA.
DR CCDS; CCDS30613.1; -.
DR RefSeq; NP_061867.1; NM_018994.2.
DR RefSeq; XP_006710761.1; XM_006710698.3.
DR AlphaFoldDB; Q6P3S6; -.
DR SMR; Q6P3S6; -.
DR BioGRID; 119962; 26.
DR IntAct; Q6P3S6; 15.
DR MINT; Q6P3S6; -.
DR STRING; 9606.ENSP00000364742; -.
DR GlyConnect; 2039; 1 N-Linked glycan (1 site).
DR GlyGen; Q6P3S6; 1 site, 2 N-linked glycans (1 site).
DR iPTMnet; Q6P3S6; -.
DR PhosphoSitePlus; Q6P3S6; -.
DR BioMuta; FBXO42; -.
DR DMDM; 51701398; -.
DR EPD; Q6P3S6; -.
DR jPOST; Q6P3S6; -.
DR MassIVE; Q6P3S6; -.
DR MaxQB; Q6P3S6; -.
DR PaxDb; Q6P3S6; -.
DR PeptideAtlas; Q6P3S6; -.
DR PRIDE; Q6P3S6; -.
DR ProteomicsDB; 66931; -.
DR Antibodypedia; 14535; 325 antibodies from 21 providers.
DR DNASU; 54455; -.
DR Ensembl; ENST00000375592.8; ENSP00000364742.3; ENSG00000037637.11.
DR GeneID; 54455; -.
DR KEGG; hsa:54455; -.
DR MANE-Select; ENST00000375592.8; ENSP00000364742.3; NM_018994.3; NP_061867.1.
DR UCSC; uc001ayg.4; human.
DR CTD; 54455; -.
DR DisGeNET; 54455; -.
DR GeneCards; FBXO42; -.
DR HGNC; HGNC:29249; FBXO42.
DR HPA; ENSG00000037637; Low tissue specificity.
DR MIM; 609109; gene.
DR neXtProt; NX_Q6P3S6; -.
DR OpenTargets; ENSG00000037637; -.
DR PharmGKB; PA134951560; -.
DR VEuPathDB; HostDB:ENSG00000037637; -.
DR eggNOG; KOG0379; Eukaryota.
DR GeneTree; ENSGT00440000039706; -.
DR HOGENOM; CLU_023579_0_0_1; -.
DR InParanoid; Q6P3S6; -.
DR OMA; EGCDLKM; -.
DR OrthoDB; 838833at2759; -.
DR PhylomeDB; Q6P3S6; -.
DR TreeFam; TF324505; -.
DR PathwayCommons; Q6P3S6; -.
DR SignaLink; Q6P3S6; -.
DR BioGRID-ORCS; 54455; 135 hits in 1128 CRISPR screens.
DR ChiTaRS; FBXO42; human.
DR GenomeRNAi; 54455; -.
DR Pharos; Q6P3S6; Tbio.
DR PRO; PR:Q6P3S6; -.
DR Proteomes; UP000005640; Chromosome 1.
DR RNAct; Q6P3S6; protein.
DR Bgee; ENSG00000037637; Expressed in hair follicle and 208 other tissues.
DR ExpressionAtlas; Q6P3S6; baseline and differential.
DR Genevisible; Q6P3S6; HS.
DR GO; GO:0019005; C:SCF ubiquitin ligase complex; IBA:GO_Central.
DR GO; GO:1990756; F:ubiquitin ligase-substrate adaptor activity; IBA:GO_Central.
DR Gene3D; 2.120.10.80; -; 1.
DR InterPro; IPR036047; F-box-like_dom_sf.
DR InterPro; IPR001810; F-box_dom.
DR InterPro; IPR015915; Kelch-typ_b-propeller.
DR Pfam; PF12937; F-box-like; 1.
DR SMART; SM00256; FBOX; 1.
DR SUPFAM; SSF117281; SSF117281; 1.
DR SUPFAM; SSF81383; SSF81383; 1.
DR PROSITE; PS50181; FBOX; 1.
PE 1: Evidence at protein level;
KW Kelch repeat; Phosphoprotein; Reference proteome; Repeat;
KW Ubl conjugation pathway.
FT CHAIN 1..717
FT /note="F-box only protein 42"
FT /id="PRO_0000119942"
FT DOMAIN 44..93
FT /note="F-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00080"
FT REPEAT 132..184
FT /note="Kelch 1"
FT REPEAT 186..242
FT /note="Kelch 2"
FT REPEAT 244..293
FT /note="Kelch 3"
FT REPEAT 295..342
FT /note="Kelch 4"
FT REGION 1..34
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 361..474
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 570..631
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 361..376
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 412..426
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 441..468
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 571..598
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 365
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18669648,
FT ECO:0007744|PubMed:23186163"
FT MOD_RES 373
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18669648,
FT ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:23186163"
FT MOD_RES 378
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:18669648"
FT MOD_RES 552
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:20068231"
FT VARIANT 471
FT /note="P -> A (in dbSNP:rs12069239)"
FT /evidence="ECO:0000269|PubMed:14702039,
FT ECO:0000269|PubMed:15489334, ECO:0000269|PubMed:17974005"
FT /id="VAR_024447"
FT CONFLICT 454
FT /note="Missing (in Ref. 3; AAH43410)"
FT /evidence="ECO:0000305"
FT CONFLICT 509
FT /note="A -> T (in Ref. 5; CAD38731)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 717 AA; 77839 MW; 938D0A018FC3DCBD CRC64;
MASSSDSEDD SFMAVDQEET VLEGTMDQDE EPHPVLEAEE TRHNRSMSEL PEEVLEYILS
FLSPYQEHKT AALVCKQWYR LIKGVAHQCY HGFMKAVQEG NIQWESRTYP YPGTPITQRF
SHSACYYDAN QSMYVFGGCT QSSCNAAFND LWRLDLNSKE WIRPLASGSY PSPKAGATLV
VYKDLLVLFG GWTRPSPYPL HQPERFFDEI HTYSPSKNWW NCIVTTHGPP PMAGHSSCVI
DDKMIVFGGS LGSRQMSNDV WVLDLEQWAW SKPNISGPSP HPRGGQSQIV IDDATILILG
GCGGPNALFK DAWLLHMHSG PWAWQPLKVE NEEHGAPELW CHPACRVGQC VVVFSQAPSG
RAPLSPSLNS RPSPISATPP ALVPETREYR SQSPVRSMDE APCVNGRWGT LRPRAQRQTP
SGSREGSLSP ARGDGSPILN GGSLSPGTAA VGGSSLDSPV QAISPSTPSA PEGYDLKIGL
SLAPRRGSLP DQKDLRLGSI DLNWDLKPAS SSNPMDGMDN RTVGGSMRHP PEQTNGVHTP
PHVASALAGA VSPGALRRSL EAIKAMSSKG PSASAALSPP LGSSPGSPGS QSLSSGETVP
IPRPGPAQGD GHSLPPIARR LGHHPPQSLN VGKPLYQSMN CKPMQMYVLD IKDTKEKGRV
KWKVFNSSSV VGPPETSLHT VVQGRGELII FGGLMDKKQN VKYYPKTNAL YFVRAKR
