FBX46_HUMAN
ID FBX46_HUMAN Reviewed; 603 AA.
AC Q6PJ61;
DT 16-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT 08-MAR-2011, sequence version 3.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=F-box only protein 46;
DE AltName: Full=F-box only protein 34-like;
GN Name=FBXO46; Synonyms=FBX46, FBXO34L;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15057824; DOI=10.1038/nature02399;
RA Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E.,
RA Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A.,
RA Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S.,
RA Carrano A.V., Caoile C., Chan Y.M., Christensen M., Cleland C.A.,
RA Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J.,
RA Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M.,
RA Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W.,
RA Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V.,
RA Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D.,
RA McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I.,
RA Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L.,
RA Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A.,
RA She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M.,
RA Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J.,
RA Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E.,
RA Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M.,
RA Rubin E.M., Lucas S.M.;
RT "The DNA sequence and biology of human chromosome 19.";
RL Nature 428:529-535(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-180.
RG The MGC Project Team;
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP RECONSTRUCTION FROM ESTS, AND CONCEPTUAL TRANSLATION OF 1-163.
RA Roechert B.;
RL Unpublished observations (APR-2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 164-603.
RC TISSUE=Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-338 AND THR-347, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=20068231; DOI=10.1126/scisignal.2000475;
RA Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L.,
RA Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.;
RT "Quantitative phosphoproteomics reveals widespread full phosphorylation
RT site occupancy during mitosis.";
RL Sci. Signal. 3:RA3-RA3(2010).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-338 AND THR-347, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
CC -!- FUNCTION: Substrate-recognition component of the SCF (SKP1-CUL1-F-box
CC protein)-type E3 ubiquitin ligase complex. {ECO:0000250}.
CC -!- SUBUNIT: Interacts with SKP1 and CUL1. {ECO:0000250}.
CC -!- INTERACTION:
CC Q6PJ61; P63208: SKP1; NbExp=4; IntAct=EBI-2322982, EBI-307486;
CC -!- CAUTION: The region from 1 to 163 was deduced from the genomic sequence
CC and ESTs by similarity to the mouse sequence. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH21978.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAH21978.1; Type=Erroneous termination; Note=Extended C-terminus.; Evidence={ECO:0000305};
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DR EMBL; AC007191; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BQ068066; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; BC021978; AAH21978.1; ALT_SEQ; mRNA.
DR CCDS; CCDS46116.1; -.
DR RefSeq; NP_001073938.1; NM_001080469.1.
DR RefSeq; NP_001316561.1; NM_001329632.1.
DR RefSeq; NP_001316562.1; NM_001329633.1.
DR RefSeq; NP_001316563.1; NM_001329634.1.
DR AlphaFoldDB; Q6PJ61; -.
DR BioGRID; 116976; 33.
DR DIP; DIP-52907N; -.
DR IntAct; Q6PJ61; 16.
DR STRING; 9606.ENSP00000410007; -.
DR iPTMnet; Q6PJ61; -.
DR PhosphoSitePlus; Q6PJ61; -.
DR BioMuta; FBXO46; -.
DR DMDM; 325511391; -.
DR EPD; Q6PJ61; -.
DR jPOST; Q6PJ61; -.
DR MassIVE; Q6PJ61; -.
DR MaxQB; Q6PJ61; -.
DR PaxDb; Q6PJ61; -.
DR PeptideAtlas; Q6PJ61; -.
DR PRIDE; Q6PJ61; -.
DR ProteomicsDB; 67191; -.
DR Antibodypedia; 31380; 73 antibodies from 14 providers.
DR DNASU; 23403; -.
DR Ensembl; ENST00000317683.4; ENSP00000410007.1; ENSG00000177051.6.
DR GeneID; 23403; -.
DR KEGG; hsa:23403; -.
DR MANE-Select; ENST00000317683.4; ENSP00000410007.1; NM_001080469.2; NP_001073938.1.
DR UCSC; uc002pcz.4; human.
DR CTD; 23403; -.
DR DisGeNET; 23403; -.
DR GeneCards; FBXO46; -.
DR HGNC; HGNC:25069; FBXO46.
DR HPA; ENSG00000177051; Low tissue specificity.
DR MIM; 609117; gene.
DR neXtProt; NX_Q6PJ61; -.
DR OpenTargets; ENSG00000177051; -.
DR VEuPathDB; HostDB:ENSG00000177051; -.
DR eggNOG; KOG4564; Eukaryota.
DR GeneTree; ENSGT00530000064222; -.
DR HOGENOM; CLU_028677_0_0_1; -.
DR InParanoid; Q6PJ61; -.
DR OMA; WNRDPLY; -.
DR OrthoDB; 329015at2759; -.
DR PhylomeDB; Q6PJ61; -.
DR TreeFam; TF331673; -.
DR PathwayCommons; Q6PJ61; -.
DR SignaLink; Q6PJ61; -.
DR BioGRID-ORCS; 23403; 13 hits in 1119 CRISPR screens.
DR ChiTaRS; FBXO46; human.
DR GenomeRNAi; 23403; -.
DR Pharos; Q6PJ61; Tdark.
DR PRO; PR:Q6PJ61; -.
DR Proteomes; UP000005640; Chromosome 19.
DR RNAct; Q6PJ61; protein.
DR Bgee; ENSG00000177051; Expressed in lower esophagus mucosa and 136 other tissues.
DR ExpressionAtlas; Q6PJ61; baseline and differential.
DR Genevisible; Q6PJ61; HS.
DR InterPro; IPR036047; F-box-like_dom_sf.
DR InterPro; IPR001810; F-box_dom.
DR InterPro; IPR039594; FBXO34/46.
DR PANTHER; PTHR16271; PTHR16271; 1.
DR Pfam; PF12937; F-box-like; 1.
DR SMART; SM00256; FBOX; 1.
DR SUPFAM; SSF81383; SSF81383; 1.
DR PROSITE; PS50181; FBOX; 1.
PE 1: Evidence at protein level;
KW Phosphoprotein; Reference proteome; Ubl conjugation pathway.
FT CHAIN 1..603
FT /note="F-box only protein 46"
FT /id="PRO_0000119950"
FT DOMAIN 470..522
FT /note="F-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00080"
FT REGION 20..63
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 111..163
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 235..301
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 326..360
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 396..440
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 125..141
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 240..255
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 342..358
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 416..430
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 338
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18669648,
FT ECO:0007744|PubMed:23186163"
FT MOD_RES 347
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:18669648,
FT ECO:0007744|PubMed:23186163"
SQ SEQUENCE 603 AA; 64631 MW; 1DF8A0985D698D2E CRC64;
MDRGSLLPFQ LWCPRPFGTY SQNQPRPPSA ALKPSACPEP GGGAEPDHGP AHSENTPPAL
ATEVPASQPA PLLSAAAAGD EGRVLLDTWY VIKPGNTKEK VAFFVAHQCG GGSRASSMKV
KGHWGSDSSK AKRRRRCLDP TKAPPDPGGR EGPPAAEEGP ASAGEDVDLL SVAEMVALVE
QRAALALQSY PRPTTPAPVV FVSAEQGGPA KGVGSERRSG GGDCSRVAEA VAHFEAQRDS
PPTKGLRKEE RPGPGPGEVR IAFRISNGRE PRAPDSGLPS GGGGRPGCAY PGSPGPGARA
KDKITCDLYQ LISPSRDALP SNVEFLLARA DEASEGDSPA PARPEDTPPA PPPPPARDCG
ASGFHVDVVV TGVVDECIFF GKDGTKNVKE ETVCLTVSPE EPPPPGQLFF LQNRGPDGPP
EPPPADSPAT APGPDDAEGT ADTSLCRLYR HVSHDFLEIR FKIQRLLEPR QYMLLLPEHV
LVKIFSFLPT RALAALKCTC HHFKGIIEAF GVRATDSRWS RDPLYRDDPC KQCRKRYEKG
DVSLCRWHPK PYHHDLPYGR SYWMCCRRAD RETPGCRLGL HDNNWVLPCN GPGGGRAGRE
EGR
