FBX50_MOUSE
ID FBX50_MOUSE Reviewed; 266 AA.
AC G3X9C2; Q8BT24;
DT 06-MAR-2013, integrated into UniProtKB/Swiss-Prot.
DT 06-MAR-2013, sequence version 2.
DT 03-AUG-2022, entry version 71.
DE RecName: Full=F-box only protein 50;
DE AltName: Full=NCC receptor protein 1;
DE Short=NCCRP-1;
DE AltName: Full=Non-specific cytotoxic cell receptor protein 1 homolog;
GN Name=Nccrp1; Synonyms=Fbxo50;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 53-266.
RC STRAIN=C57BL/6J; TISSUE=Embryo;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-31; SER-37; SER-40; SER-43
RP AND THR-46, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney, and Lung;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=22087255; DOI=10.1371/journal.pone.0027152;
RA Kallio H., Tolvanen M., Janis J., Pan P.W., Laurila E., Kallioniemi A.,
RA Kilpinen S., Tuominen V.J., Isola J., Valjakka J., Pastorekova S.,
RA Pastorek J., Parkkila S.;
RT "Characterization of non-specific cytotoxic cell receptor protein 1: a new
RT member of the lectin-type subfamily of F-box proteins.";
RL PLoS ONE 6:E27152-E27152(2011).
CC -!- FUNCTION: Promotes cell proliferation. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Strongly expressed in kidney. Weakly expressed in
CC stomach, colon, duodenum and prostate. {ECO:0000269|PubMed:22087255}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EDL24129.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AC136456; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466593; EDL24129.1; ALT_INIT; Genomic_DNA.
DR EMBL; AK028022; BAC25704.1; -; mRNA.
DR CCDS; CCDS39862.2; -.
DR RefSeq; NP_001074584.1; NM_001081115.1.
DR AlphaFoldDB; G3X9C2; -.
DR SMR; G3X9C2; -.
DR BioGRID; 231354; 4.
DR IntAct; G3X9C2; 1.
DR MINT; G3X9C2; -.
DR STRING; 10090.ENSMUSP00000055562; -.
DR iPTMnet; G3X9C2; -.
DR PhosphoSitePlus; G3X9C2; -.
DR jPOST; G3X9C2; -.
DR MaxQB; G3X9C2; -.
DR PaxDb; G3X9C2; -.
DR PeptideAtlas; G3X9C2; -.
DR PRIDE; G3X9C2; -.
DR ProteomicsDB; 271886; -.
DR Antibodypedia; 45009; 73 antibodies from 17 providers.
DR Ensembl; ENSMUST00000239002; ENSMUSP00000159104; ENSMUSG00000047586.
DR GeneID; 233038; -.
DR KEGG; mmu:233038; -.
DR CTD; 342897; -.
DR MGI; MGI:2685009; Nccrp1.
DR VEuPathDB; HostDB:ENSMUSG00000047586; -.
DR eggNOG; KOG3248; Eukaryota.
DR GeneTree; ENSGT00940000161313; -.
DR InParanoid; G3X9C2; -.
DR OrthoDB; 922544at2759; -.
DR TreeFam; TF320527; -.
DR BioGRID-ORCS; 233038; 4 hits in 72 CRISPR screens.
DR ChiTaRS; Nccrp1; mouse.
DR PRO; PR:G3X9C2; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; G3X9C2; protein.
DR Bgee; ENSMUSG00000047586; Expressed in esophagus and 24 other tissues.
DR ExpressionAtlas; G3X9C2; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0019005; C:SCF ubiquitin ligase complex; IBA:GO_Central.
DR GO; GO:0006516; P:glycoprotein catabolic process; IBA:GO_Central.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; ISO:MGI.
DR GO; GO:0016567; P:protein ubiquitination; IEA:GOC.
DR GO; GO:0031146; P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR GO; GO:0030433; P:ubiquitin-dependent ERAD pathway; IBA:GO_Central.
DR InterPro; IPR007397; F-box-assoc_dom.
DR InterPro; IPR039752; F-box_only.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR PANTHER; PTHR12125; PTHR12125; 1.
DR Pfam; PF04300; FBA; 1.
DR SMART; SM01198; FBA; 1.
DR SUPFAM; SSF49785; SSF49785; 1.
DR PROSITE; PS51114; FBA; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Phosphoprotein; Reference proteome.
FT CHAIN 1..266
FT /note="F-box only protein 50"
FT /id="PRO_0000421450"
FT DOMAIN 86..264
FT /note="FBA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00482"
FT REGION 1..53
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 26..51
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 31
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 37
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 40
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 43
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 46
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT CONFLICT 53
FT /note="N -> D (in Ref. 3; BAC25704)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 266 AA; 30408 MW; 23E1532A240EE700 CRC64;
MEKTQDRDTL SGRMEAEGSL NSEELPPHPQ SPPPPPSPRS PTSPVTPELP QPNAPTEVEA
RQLLVEEWGP LSGKLELPPR ISWQLLFLER PLYRNLLSSP NPEGINIYQP APPTGPTRKP
LKELGNFRGW YITTQNLQGP LSWTVKEQCV NLLAKKLWEE LLDDEQPDIT IMDWFEDSRL
DQCVYELHVW LLAADRRTVI AQHHVAPRTN GRGPPGRWIQ VSHVFRQYGP GVRFVYFQHK
AKNRMEPGGL RRTRVTDSSV SVQLRE
