FBXL4_MOUSE
ID FBXL4_MOUSE Reviewed; 621 AA.
AC Q8BH70;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=F-box/LRR-repeat protein 4;
DE AltName: Full=F-box and leucine-rich repeat protein 4;
GN Name=Fbxl4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Olfactory bulb, and Spinal ganglion;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, and Kidney;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [4]
RP METHYLATION [LARGE SCALE ANALYSIS] AT ARG-28, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Embryo;
RX PubMed=24129315; DOI=10.1074/mcp.o113.027870;
RA Guo A., Gu H., Zhou J., Mulhern D., Wang Y., Lee K.A., Yang V., Aguiar M.,
RA Kornhauser J., Jia X., Ren J., Beausoleil S.A., Silva J.C., Vemulapalli V.,
RA Bedford M.T., Comb M.J.;
RT "Immunoaffinity enrichment and mass spectrometry analysis of protein
RT methylation.";
RL Mol. Cell. Proteomics 13:372-387(2014).
CC -!- SUBUNIT: Part of a SCF (SKP1-cullin-F-box) protein ligase complex.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC Mitochondrion {ECO:0000250}.
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DR EMBL; AK032391; BAC27850.1; -; mRNA.
DR EMBL; AK051429; BAC34636.1; -; mRNA.
DR EMBL; AL683854; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL772230; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS18004.1; -.
DR RefSeq; NP_766576.1; NM_172988.4.
DR RefSeq; XP_006538016.1; XM_006537953.2.
DR AlphaFoldDB; Q8BH70; -.
DR SMR; Q8BH70; -.
DR BioGRID; 234659; 1.
DR STRING; 10090.ENSMUSP00000042219; -.
DR iPTMnet; Q8BH70; -.
DR PhosphoSitePlus; Q8BH70; -.
DR MaxQB; Q8BH70; -.
DR PaxDb; Q8BH70; -.
DR PRIDE; Q8BH70; -.
DR ProteomicsDB; 267356; -.
DR Antibodypedia; 18894; 108 antibodies from 19 providers.
DR DNASU; 269514; -.
DR Ensembl; ENSMUST00000039234; ENSMUSP00000042219; ENSMUSG00000040410.
DR GeneID; 269514; -.
DR KEGG; mmu:269514; -.
DR UCSC; uc008sdo.2; mouse.
DR CTD; 26235; -.
DR MGI; MGI:2140367; Fbxl4.
DR VEuPathDB; HostDB:ENSMUSG00000040410; -.
DR eggNOG; KOG1947; Eukaryota.
DR GeneTree; ENSGT00940000155184; -.
DR HOGENOM; CLU_024764_2_0_1; -.
DR InParanoid; Q8BH70; -.
DR OMA; GWCMREA; -.
DR OrthoDB; 672780at2759; -.
DR PhylomeDB; Q8BH70; -.
DR TreeFam; TF323721; -.
DR Reactome; R-MMU-8951664; Neddylation.
DR Reactome; R-MMU-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR BioGRID-ORCS; 269514; 5 hits in 73 CRISPR screens.
DR ChiTaRS; Fbxl4; mouse.
DR PRO; PR:Q8BH70; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q8BH70; protein.
DR Bgee; ENSMUSG00000040410; Expressed in superior cervical ganglion and 217 other tissues.
DR ExpressionAtlas; Q8BH70; baseline and differential.
DR Genevisible; Q8BH70; MM.
DR GO; GO:0005758; C:mitochondrial intermembrane space; ISS:UniProtKB.
DR GO; GO:0016607; C:nuclear speck; ISO:MGI.
DR GO; GO:0019005; C:SCF ubiquitin ligase complex; IBA:GO_Central.
DR GO; GO:0000422; P:autophagy of mitochondrion; IMP:MGI.
DR GO; GO:0031146; P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR036047; F-box-like_dom_sf.
DR InterPro; IPR001810; F-box_dom.
DR InterPro; IPR006553; Leu-rich_rpt_Cys-con_subtyp.
DR InterPro; IPR032675; LRR_dom_sf.
DR Pfam; PF00646; F-box; 1.
DR SMART; SM00256; FBOX; 1.
DR SMART; SM00367; LRR_CC; 7.
DR SUPFAM; SSF81383; SSF81383; 1.
DR PROSITE; PS50181; FBOX; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Leucine-rich repeat; Methylation; Mitochondrion; Nucleus;
KW Reference proteome; Repeat; Ubl conjugation pathway.
FT CHAIN 1..621
FT /note="F-box/LRR-repeat protein 4"
FT /id="PRO_0000307719"
FT DOMAIN 277..332
FT /note="F-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00080"
FT REPEAT 376..397
FT /note="LRR 1"
FT REPEAT 402..421
FT /note="LRR 2"
FT REPEAT 427..448
FT /note="LRR 3"
FT REPEAT 452..474
FT /note="LRR 4"
FT REPEAT 480..501
FT /note="LRR 5"
FT REPEAT 504..524
FT /note="LRR 6"
FT REPEAT 532..558
FT /note="LRR 7"
FT REPEAT 559..583
FT /note="LRR 8"
FT REPEAT 584..609
FT /note="LRR 9"
FT MOD_RES 28
FT /note="Asymmetric dimethylarginine"
FT /evidence="ECO:0007744|PubMed:24129315"
SQ SEQUENCE 621 AA; 70269 MW; 458544AD66711119 CRC64;
MSPVFPMLTV LTMFYYMCLR RRARTATRGD MMNSHRTIVS NSRTSPLNAE VVQYAKEVVD
FSSHYGSENS MSYTMWNLAG VPNVFPSSGD FTQTAVFRTY GTWWDQCPSA SLPFRRTPSS
FQSQDYVELT FEQQVYPTAV HVLETYHPGA VIRILACSAN PYSPNPPAEV RWEILWSERP
MKVNASQARQ FKPCIKQINF PTNLIRLEVN SSLLDYYTEL DAVVLHGTKD KPLLSLKTAL
VDMNDLEDDD YEEKDGCEMD ALNKKFSSAA LGDGPHNGYF DKLPYELIQL ILNHLSLPDL
CRLAQTCRLL HQHCCDPLQY IHLNLQPYWA RLDDTSLEFL QARCVLVQWL NLSWTGNRGF
ISVSGFSRFL KVCGSELVRL ELSCSHFLND TCLEVISEMC PNLQDLNLSS CDKLPPQAFG
HIAKLCSLKR LVLYRTKVEQ TALLSILNFC AELQHLSLGS CVMIEDYDVI ASMIGAKCKN
LRTLDLWRCK NITENGIAEL ASGCVLLEEL DLGWCPTLQS STGCFVRLAR QLPNLQKLFL
TANRSVCDTD IEELASNCTR LQQLDILGTR MVSPASLRKL LESCKDLSLL DVSFCSQIDN
KAVLELNASF PKVFIKKSFT Q
