FBXL_CAEEL
ID FBXL_CAEEL Reviewed; 466 AA.
AC P34284; Q8T3G0;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 25-OCT-2005, sequence version 3.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=F-box/LRR-repeat protein fbxl-1 {ECO:0000305};
DE AltName: Full=F-box and leucine-rich repeat protein 1 {ECO:0000312|WormBase:C02F5.7b};
GN Name=fbxl-1 {ECO:0000312|WormBase:C02F5.7b};
GN Synonyms=cfl-1 {ECO:0000303|Ref.3, ECO:0000312|WormBase:C02F5.7b};
GN ORFNames=C02F5.7 {ECO:0000312|WormBase:C02F5.7b};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=7906398; DOI=10.1038/368032a0;
RA Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J.,
RA Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M.,
RA Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L., Gardner A.,
RA Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M.,
RA Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C.,
RA Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A.,
RA Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M.,
RA Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K.,
RA Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L.,
RA Wilkinson-Sproat J., Wohldman P.;
RT "2.2 Mb of contiguous nucleotide sequence from chromosome III of C.
RT elegans.";
RL Nature 368:32-38(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RX DOI=10.1080/19768354.2012.665612;
RA Kim S.M., Jang S.H., Son N., Han C.T., Min K., Lee H., Hwang S.Y.;
RT "A novel F-box protein with leucine-rich repeats affects defecation
RT frequency and daumone response in Caenorhabditis elegans.";
RL Anim. Cells Syst. (Seoul) 16:280-288(2012).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=30460068; DOI=10.1080/19768354.2017.1325779;
RA Kim S.M., Hwang S.Y.;
RT "CFL-1, a novel F-box protein with leucine-rich repeat may interact with
RT UNC-10 for the regulation of defecation and daumone response in
RT Caenorhabditis elegans.";
RL Anim. Cells Syst. (Seoul) 21:185-189(2017).
CC -!- FUNCTION: Substrate-recognition component of the SCF (SKP1-CUL1-F-box
CC protein)-type E3 ubiquitin ligase complex (By similarity). Plays a role
CC in regulating the entry into the dauer state (Ref.3, PubMed:30460068).
CC In hermaphrodites, may play a role in modulating the rate of defecation
CC (Ref.3, PubMed:30460068). {ECO:0000250|UniProtKB:Q9CZV8,
CC ECO:0000269|PubMed:30460068, ECO:0000269|Ref.3}.
CC -!- SUBUNIT: Component of the SCF (SKP1-CUL1-F-box protein)-type E3
CC ubiquitin ligase complex. {ECO:0000250|UniProtKB:Q9CZV8}.
CC -!- SUBCELLULAR LOCATION: Perikaryon {ECO:0000269|Ref.3}. Cell projection,
CC dendrite {ECO:0000269|Ref.3}. Cell projection, cilium
CC {ECO:0000269|Ref.3}. Cell projection, axon {ECO:0000269|Ref.3}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=b {ECO:0000312|WormBase:C02F5.7b};
CC IsoId=P34284-1; Sequence=Displayed;
CC Name=a {ECO:0000312|WormBase:C02F5.7b};
CC IsoId=P34284-2; Sequence=VSP_015950;
CC -!- TISSUE SPECIFICITY: Expressed in neuroglial cells such as the socket
CC cell and sheath cell, neurosecretory motor neurons and regions around
CC the pharynx and anus. {ECO:0000269|Ref.3}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown reduces the rate of dauer
CC formation following treatment with daumone, a pheromone which induces
CC the dauer state (Ref.3, PubMed:30460068). RNAi-mediated knockdown
CC results in a 10-20% increase in the average time interval between
CC defecations in hermaphrodites (Ref.3, PubMed:30460068).
CC {ECO:0000269|PubMed:30460068, ECO:0000269|Ref.3}.
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DR EMBL; BX284603; CCD62632.1; -; Genomic_DNA.
DR EMBL; BX284603; CCD62633.1; -; Genomic_DNA.
DR PIR; G88523; G88523.
DR PIR; S44609; S44609.
DR RefSeq; NP_741248.1; NM_171210.4. [P34284-1]
DR RefSeq; NP_741249.1; NM_171211.3.
DR AlphaFoldDB; P34284; -.
DR SMR; P34284; -.
DR BioGRID; 532123; 4.
DR IntAct; P34284; 2.
DR MINT; P34284; -.
DR STRING; 6239.C02F5.7b.2; -.
DR EPD; P34284; -.
DR PaxDb; P34284; -.
DR PeptideAtlas; P34284; -.
DR PRIDE; P34284; -.
DR EnsemblMetazoa; C02F5.7a.1; C02F5.7a.1; WBGene00015350. [P34284-2]
DR EnsemblMetazoa; C02F5.7a.2; C02F5.7a.2; WBGene00015350. [P34284-2]
DR EnsemblMetazoa; C02F5.7b.1; C02F5.7b.1; WBGene00015350. [P34284-1]
DR GeneID; 3564805; -.
DR KEGG; cel:CELE_C02F5.7; -.
DR UCSC; C02F5.7a.1; c. elegans. [P34284-1]
DR CTD; 3564805; -.
DR WormBase; C02F5.7a; CE24780; WBGene00015350; fbxl-1. [P34284-2]
DR WormBase; C02F5.7b; CE30418; WBGene00015350; fbxl-1. [P34284-1]
DR eggNOG; KOG4341; Eukaryota.
DR GeneTree; ENSGT00940000170586; -.
DR HOGENOM; CLU_016072_7_1_1; -.
DR InParanoid; P34284; -.
DR OMA; LCNRIRY; -.
DR OrthoDB; 1046098at2759; -.
DR PhylomeDB; P34284; -.
DR Reactome; R-CEL-8951664; Neddylation.
DR Reactome; R-CEL-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR PRO; PR:P34284; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00015350; Expressed in pharyngeal muscle cell (C elegans) and 4 other tissues.
DR GO; GO:0030424; C:axon; IDA:UniProtKB.
DR GO; GO:0005929; C:cilium; IDA:UniProtKB.
DR GO; GO:0030425; C:dendrite; IDA:UniProtKB.
DR GO; GO:0043204; C:perikaryon; IDA:UniProtKB.
DR GO; GO:0019005; C:SCF ubiquitin ligase complex; IBA:GO_Central.
DR GO; GO:0043053; P:dauer entry; IMP:WormBase.
DR GO; GO:0035882; P:defecation rhythm; IMP:WormBase.
DR GO; GO:1905909; P:regulation of dauer entry; IMP:UniProtKB.
DR GO; GO:2000746; P:regulation of defecation rhythm; IMP:UniProtKB.
DR GO; GO:0031146; P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR Gene3D; 3.80.10.10; -; 2.
DR InterPro; IPR001810; F-box_dom.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR006553; Leu-rich_rpt_Cys-con_subtyp.
DR InterPro; IPR032675; LRR_dom_sf.
DR Pfam; PF12937; F-box-like; 1.
DR Pfam; PF13516; LRR_6; 3.
DR SMART; SM00256; FBOX; 1.
DR SMART; SM00367; LRR_CC; 11.
DR PROSITE; PS50181; FBOX; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell projection; Leucine-rich repeat;
KW Reference proteome; Repeat; Ubl conjugation pathway.
FT CHAIN 1..466
FT /note="F-box/LRR-repeat protein fbxl-1"
FT /id="PRO_0000119981"
FT DOMAIN 54..100
FT /note="F-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00080"
FT REPEAT 122..147
FT /note="LRR 1"
FT REPEAT 148..173
FT /note="LRR 2"
FT REPEAT 174..199
FT /note="LRR 3"
FT REPEAT 200..225
FT /note="LRR 4"
FT REPEAT 226..251
FT /note="LRR 5"
FT REPEAT 252..277
FT /note="LRR 6"
FT REPEAT 278..303
FT /note="LRR 7"
FT REPEAT 304..329
FT /note="LRR 8"
FT REPEAT 330..355
FT /note="LRR 9"
FT REPEAT 356..381
FT /note="LRR 10"
FT REPEAT 408..433
FT /note="LRR 11"
FT VAR_SEQ 459..466
FT /note="ICRCCVIL -> MLF (in isoform a)"
FT /evidence="ECO:0000305"
FT /id="VSP_015950"
SQ SEQUENCE 466 AA; 52065 MW; 05BCE82AA606E15F CRC64;
METAERISAA ASAASSRRAK RLAQQAHKTH PVIQAKQNQM YLITTLSPAQ VDNSLINRVL
PKEVLLKVFS FLDTKALCRS AQVCRSWSIL ALDGSNWQRV DLFTFQRDVK TAVVENLARR
CGGFLKELSL KGCENVHDSA LRTFTSRCPN LEHLSLYRCK RVTDASCENL GRYCHKLNYL
NLENCSSITD RAMKYIGDGC PNLSYLNISW CDAIQDRGVQ IILSNCKSLD TLILRGCEGL
TENVFGSVEA HMGAIKKLNL LQCFQLTDIT VQNIANGATA LEYLCMSNCN QISDRSLVSL
GQHSHNLKVL ELSGCTLLGD NGFIPLARGC RQLERLDMED CSLISDHTIN SLANNCTALR
ELSLSHCELI TDESIQNLAS KHRETLNVLE LDNCPQLTDS TLSHLRHCKA LKRIDLYDCQ
NVSKEAIVRF QHHRPNIEIH AYFAPVTPPT DQVVNRGGIC RCCVIL
