FBXW4_MOUSE
ID FBXW4_MOUSE Reviewed; 410 AA.
AC Q9JMJ2; Q9QXW3;
DT 21-FEB-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=F-box/WD repeat-containing protein 4;
DE AltName: Full=F-box and WD-40 domain-containing protein 4;
DE AltName: Full=Protein hagoromo;
GN Name=Fbxw4; Synonyms=Fbw4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=10801422; DOI=10.1016/s0960-9822(00)00444-9;
RA Kawakami K., Amsterdam A., Shimoda N., Becker T., Mugg J., Shima A.,
RA Hopkins N.;
RT "Proviral insertions in the zebrafish hagoromo gene, encoding an F-
RT box/WD40-repeat protein, cause stripe pattern anomalies.";
RL Curr. Biol. 10:463-466(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=10531037; DOI=10.1016/s0960-9822(00)80021-4;
RA Winston J.T., Koepp D.M., Zhu C., Elledge S.J., Harper J.W.;
RT "A family of mammalian F-box proteins.";
RL Curr. Biol. 9:1180-1182(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Retina;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP INTERACTION WITH POUF51.
RX PubMed=29153991; DOI=10.1016/j.stemcr.2017.10.017;
RA Bae K.B., Yu D.H., Lee K.Y., Yao K., Ryu J., Lim D.Y., Zykova T.A.,
RA Kim M.O., Bode A.M., Dong Z.;
RT "Serine 347 Phosphorylation by JNKs Negatively Regulates OCT4 Protein
RT Stability in Mouse Embryonic Stem Cells.";
RL Stem Cell Reports 9:2050-2064(2017).
CC -!- FUNCTION: Probably recognizes and binds to some phosphorylated proteins
CC and promotes their ubiquitination and degradation. Likely to be
CC involved in key signaling pathways crucial for normal limb development.
CC May participate in Wnt signaling.
CC -!- SUBUNIT: Part of a SCF (SKP1-cullin-F-box) protein ligase complex
CC (Probable). Interacts with POUF51 (PubMed:29153991).
CC {ECO:0000269|PubMed:29153991, ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF22827.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AB022163; BAA94666.1; -; mRNA.
DR EMBL; AF176519; AAF22827.1; ALT_FRAME; mRNA.
DR EMBL; BC027031; AAH27031.1; -; mRNA.
DR CCDS; CCDS29863.1; -.
DR RefSeq; NP_038935.1; NM_013907.2.
DR AlphaFoldDB; Q9JMJ2; -.
DR SMR; Q9JMJ2; -.
DR BioGRID; 205975; 7.
DR STRING; 10090.ENSMUSP00000036505; -.
DR PhosphoSitePlus; Q9JMJ2; -.
DR PaxDb; Q9JMJ2; -.
DR PRIDE; Q9JMJ2; -.
DR ProteomicsDB; 267361; -.
DR Antibodypedia; 31311; 232 antibodies from 27 providers.
DR DNASU; 30838; -.
DR Ensembl; ENSMUST00000046869; ENSMUSP00000036505; ENSMUSG00000040913.
DR GeneID; 30838; -.
DR KEGG; mmu:30838; -.
DR UCSC; uc008hrf.1; mouse.
DR CTD; 6468; -.
DR MGI; MGI:1354698; Fbxw4.
DR VEuPathDB; HostDB:ENSMUSG00000040913; -.
DR eggNOG; ENOG502QV15; Eukaryota.
DR GeneTree; ENSGT00390000005029; -.
DR HOGENOM; CLU_034660_0_0_1; -.
DR InParanoid; Q9JMJ2; -.
DR OMA; ITEQRMH; -.
DR OrthoDB; 730077at2759; -.
DR PhylomeDB; Q9JMJ2; -.
DR TreeFam; TF325020; -.
DR Reactome; R-MMU-8951664; Neddylation.
DR Reactome; R-MMU-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR BioGRID-ORCS; 30838; 2 hits in 72 CRISPR screens.
DR ChiTaRS; Fbxw4; mouse.
DR PRO; PR:Q9JMJ2; -.
DR Proteomes; UP000000589; Chromosome 19.
DR RNAct; Q9JMJ2; protein.
DR Bgee; ENSMUSG00000040913; Expressed in secondary oocyte and 248 other tissues.
DR ExpressionAtlas; Q9JMJ2; baseline and differential.
DR Genevisible; Q9JMJ2; MM.
DR GO; GO:0019005; C:SCF ubiquitin ligase complex; IBA:GO_Central.
DR GO; GO:0051216; P:cartilage development; IMP:MGI.
DR GO; GO:0042733; P:embryonic digit morphogenesis; IMP:MGI.
DR GO; GO:0060173; P:limb development; IMP:MGI.
DR GO; GO:0002053; P:positive regulation of mesenchymal cell proliferation; IMP:MGI.
DR GO; GO:0031146; P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR GO; GO:0016055; P:Wnt signaling pathway; IEA:UniProtKB-KW.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR036047; F-box-like_dom_sf.
DR InterPro; IPR001810; F-box_dom.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF12937; F-box-like; 1.
DR Pfam; PF00400; WD40; 1.
DR SMART; SM00320; WD40; 5.
DR SUPFAM; SSF50978; SSF50978; 1.
DR SUPFAM; SSF81383; SSF81383; 1.
DR PROSITE; PS50181; FBOX; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Reference proteome; Repeat; Ubl conjugation pathway;
KW WD repeat; Wnt signaling pathway.
FT CHAIN 1..410
FT /note="F-box/WD repeat-containing protein 4"
FT /id="PRO_0000050991"
FT DOMAIN 23..69
FT /note="F-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00080"
FT REPEAT 159..196
FT /note="WD 1"
FT REPEAT 198..235
FT /note="WD 2"
FT REPEAT 289..327
FT /note="WD 3"
FT REPEAT 333..372
FT /note="WD 4"
FT CONFLICT 39
FT /note="S -> F (in Ref. 2; AAF22827)"
FT /evidence="ECO:0000305"
FT CONFLICT 47..50
FT /note="GRLA -> APG (in Ref. 2; AAF22827)"
FT /evidence="ECO:0000305"
FT CONFLICT 58
FT /note="R -> A (in Ref. 2; AAF22827)"
FT /evidence="ECO:0000305"
FT CONFLICT 185
FT /note="V -> I (in Ref. 2; AAF22827)"
FT /evidence="ECO:0000305"
FT CONFLICT 218
FT /note="R -> M (in Ref. 2; AAF22827)"
FT /evidence="ECO:0000305"
FT CONFLICT 222..230
FT /note="AKVWPLASG -> SKVPFAASV (in Ref. 2)"
FT /evidence="ECO:0000305"
FT CONFLICT 241
FT /note="T -> I (in Ref. 2)"
FT /evidence="ECO:0000305"
FT CONFLICT 249
FT /note="A -> V (in Ref. 2)"
FT /evidence="ECO:0000305"
FT CONFLICT 273
FT /note="W -> C (in Ref. 2)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 410 AA; 46142 MW; 01BC047352B184C6 CRC64;
MAEDAAEDAA AAAVEPATRP AAGPALWRLP EELLLLICSY LDTRALGRLA QVCRWLRRFT
SCDLLWRPIA RASLNTGFTR LGTDLMAGIP VKERVKLSQN WRLGRCRDRI LLKWRYSQMP
WMQLQDASLY LSQANFILAY QFRPDGASLN RRPFRVFSGH DEDVCHFVLA NSHIVSAGGD
GKIGVHKIHS TFTVKYSAHE QEVNCVDCKG GIIVSGSRDR TAKVWPLASG RLGQCLHTIQ
TEDRVWSIAI SPLLSSFVTG TACCGHFSPL RIWDLNSGQL ITHLGSDFPP GAGVLDVMYE
SPSTLLSCGY DTYVRYWDLR TSTRKCVMEW EEPHDSTFYC LQTDGNHLLA TGSSYYGLVR
LWDRRQRACL HAFSLTSTPL SSPVYCLRFT TRHLYAALSY NLHVLDFQNP
