FBXW9_HUMAN
ID FBXW9_HUMAN Reviewed; 458 AA.
AC Q5XUX1; B3KVP7; Q9BT89;
DT 01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 07-OCT-2020, sequence version 3.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=F-box/WD repeat-containing protein 9;
DE AltName: Full=F-box and WD-40 domain-containing protein 9;
GN Name=FBXW9; Synonyms=FBW9;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RX PubMed=15520277; DOI=10.1101/gad.1255304;
RA Jin J., Cardozo T., Lovering R.C., Elledge S.J., Pagano M., Harper J.W.;
RT "Systematic analysis and nomenclature of mammalian F-box proteins.";
RL Genes Dev. 18:2573-2580(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15057824; DOI=10.1038/nature02399;
RA Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E.,
RA Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A.,
RA Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S.,
RA Carrano A.V., Caoile C., Chan Y.M., Christensen M., Cleland C.A.,
RA Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J.,
RA Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M.,
RA Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W.,
RA Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V.,
RA Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D.,
RA McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I.,
RA Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L.,
RA Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A.,
RA She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M.,
RA Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J.,
RA Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E.,
RA Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M.,
RA Rubin E.M., Lucas S.M.;
RT "The DNA sequence and biology of human chromosome 19.";
RL Nature 428:529-535(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Lymph;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18; THR-55 AND SER-59, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Leukemic T-cell;
RX PubMed=19690332; DOI=10.1126/scisignal.2000007;
RA Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,
RA Rodionov V., Han D.K.;
RT "Quantitative phosphoproteomic analysis of T cell receptor signaling
RT reveals system-wide modulation of protein-protein interactions.";
RL Sci. Signal. 2:RA46-RA46(2009).
RN [7]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18; THR-55 AND SER-59, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma, and Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
CC -!- FUNCTION: Substrate-recognition component of the SCF (SKP1-CUL1-F-box
CC protein)-type E3 ubiquitin ligase complex. {ECO:0000250}.
CC -!- SUBUNIT: Interacts with SKP1 and CUL1. {ECO:0000250}.
CC -!- INTERACTION:
CC Q5XUX1; P63208: SKP1; NbExp=4; IntAct=EBI-2322729, EBI-307486;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q5XUX1-3; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5XUX1-2; Sequence=VSP_060706, VSP_060707;
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DR EMBL; AY736034; AAU50678.1; -; mRNA.
DR EMBL; AK123037; BAG53859.1; -; mRNA.
DR EMBL; AC018761; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC004290; -; NOT_ANNOTATED_CDS; mRNA.
DR CCDS; CCDS12278.2; -. [Q5XUX1-3]
DR RefSeq; NP_115677.2; NM_032301.2. [Q5XUX1-3]
DR AlphaFoldDB; Q5XUX1; -.
DR SMR; Q5XUX1; -.
DR BioGRID; 123988; 28.
DR IntAct; Q5XUX1; 5.
DR STRING; 9606.ENSP00000376945; -.
DR iPTMnet; Q5XUX1; -.
DR PhosphoSitePlus; Q5XUX1; -.
DR BioMuta; FBXW9; -.
DR DMDM; 115311596; -.
DR EPD; Q5XUX1; -.
DR jPOST; Q5XUX1; -.
DR MassIVE; Q5XUX1; -.
DR MaxQB; Q5XUX1; -.
DR PeptideAtlas; Q5XUX1; -.
DR PRIDE; Q5XUX1; -.
DR ProteomicsDB; 65848; -. [Q5XUX1-2]
DR ProteomicsDB; 65849; -. [Q5XUX1-3]
DR Antibodypedia; 26106; 107 antibodies from 16 providers.
DR DNASU; 84261; -.
DR Ensembl; ENST00000393261.8; ENSP00000376945.3; ENSG00000132004.13. [Q5XUX1-3]
DR Ensembl; ENST00000587955.1; ENSP00000465387.1; ENSG00000132004.13. [Q5XUX1-2]
DR GeneID; 84261; -.
DR KEGG; hsa:84261; -.
DR MANE-Select; ENST00000393261.8; ENSP00000376945.3; NM_032301.3; NP_115677.2.
DR UCSC; uc002mum.2; human. [Q5XUX1-3]
DR CTD; 84261; -.
DR GeneCards; FBXW9; -.
DR HGNC; HGNC:28136; FBXW9.
DR HPA; ENSG00000132004; Low tissue specificity.
DR MIM; 609074; gene.
DR neXtProt; NX_Q5XUX1; -.
DR OpenTargets; ENSG00000132004; -.
DR PharmGKB; PA134910095; -.
DR VEuPathDB; HostDB:ENSG00000132004; -.
DR eggNOG; KOG0274; Eukaryota.
DR GeneTree; ENSGT00390000006806; -.
DR HOGENOM; CLU_034344_0_0_1; -.
DR InParanoid; Q5XUX1; -.
DR OMA; MSYSGCE; -.
DR OrthoDB; 1109214at2759; -.
DR PhylomeDB; Q5XUX1; -.
DR TreeFam; TF313746; -.
DR PathwayCommons; Q5XUX1; -.
DR Reactome; R-HSA-390471; Association of TriC/CCT with target proteins during biosynthesis.
DR Reactome; R-HSA-8951664; Neddylation.
DR Reactome; R-HSA-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR SignaLink; Q5XUX1; -.
DR BioGRID-ORCS; 84261; 17 hits in 1110 CRISPR screens.
DR ChiTaRS; FBXW9; human.
DR GenomeRNAi; 84261; -.
DR Pharos; Q5XUX1; Tdark.
DR PRO; PR:Q5XUX1; -.
DR Proteomes; UP000005640; Chromosome 19.
DR RNAct; Q5XUX1; protein.
DR Bgee; ENSG00000132004; Expressed in right uterine tube and 131 other tissues.
DR ExpressionAtlas; Q5XUX1; baseline and differential.
DR Genevisible; Q5XUX1; HS.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR036047; F-box-like_dom_sf.
DR InterPro; IPR001810; F-box_dom.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF12937; F-box-like; 1.
DR Pfam; PF00400; WD40; 2.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00256; FBOX; 1.
DR SMART; SM00320; WD40; 6.
DR SUPFAM; SSF50978; SSF50978; 1.
DR SUPFAM; SSF81383; SSF81383; 1.
DR PROSITE; PS50181; FBOX; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 2.
DR PROSITE; PS50082; WD_REPEATS_2; 3.
DR PROSITE; PS50294; WD_REPEATS_REGION; 2.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Phosphoprotein; Reference proteome;
KW Repeat; Ubl conjugation pathway; WD repeat.
FT CHAIN 1..458
FT /note="F-box/WD repeat-containing protein 9"
FT /id="PRO_0000050999"
FT DOMAIN 76..123
FT /note="F-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00080"
FT REPEAT 171..210
FT /note="WD 1"
FT /evidence="ECO:0000255"
FT REPEAT 220..261
FT /note="WD 2"
FT /evidence="ECO:0000255"
FT REPEAT 264..301
FT /note="WD 3"
FT /evidence="ECO:0000255"
FT REPEAT 305..342
FT /note="WD 4"
FT /evidence="ECO:0000255"
FT REPEAT 344..381
FT /note="WD 5"
FT /evidence="ECO:0000255"
FT REPEAT 387..424
FT /note="WD 6"
FT /evidence="ECO:0000255"
FT REPEAT 427..458
FT /note="WD 7"
FT /evidence="ECO:0000255"
FT REGION 1..30
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 42..64
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 45..64
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0007744|PubMed:22814378"
FT MOD_RES 18
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19690332,
FT ECO:0007744|PubMed:23186163, ECO:0007744|PubMed:24275569"
FT MOD_RES 55
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:19690332,
FT ECO:0007744|PubMed:23186163"
FT MOD_RES 59
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19690332,
FT ECO:0007744|PubMed:23186163"
FT VAR_SEQ 128..137
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_060706"
FT VAR_SEQ 294
FT /note="R -> RDRMETRDALMGWGGPSRGSDIPPHRPITHE (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_060707"
FT VARIANT 70
FT /note="R -> K (in dbSNP:rs6511833)"
FT /id="VAR_057599"
FT VARIANT 219
FT /note="T -> A (in dbSNP:rs10424623)"
FT /id="VAR_062097"
SQ SEQUENCE 458 AA; 50760 MW; A2A5B378211A3D06 CRC64;
MELPLGRCDD SRTWDDDSDP ESETDPDAQA KAYVARVLSP PKSGLAFSRP SQLSTPAASP
SASEPRAASR VSAVSEPGLL SLPPELLLEI CSYLDARLVL HVLSRVCHAL RDLVSDHVTW
RLRALRRVRA PYPVVEEKNF DWPAACIALE QHLSRWAEDG RWVEYFCLAE GHVASVDSVL
LLQGGSLCLS GSRDRNVNLW DLRQLGTESN QVLIKTLGTK RNSTHEGWVW SLAAQDHRVC
SGSWDSTVKL WDMAADGQQF GEIKASSAVL CLSYLPDILV TGTYDKKVTI YDPRAGPALL
KHQQLHSRPV LTLLADDRHI ISGSEDHTLV VVDRRANSVL QRLQLDSYLL CMSYQEPQLW
AGDNQGLLHV FANRNGCFQL IRSFDVGHSF PITGIQYSVG ALYTTSTDKT IRVHVPTDPP
RTICTRRHDN GLNRVCAEGN LVVAGSGDLS LEVWRLQA
