FCA2_TRYBB
ID FCA2_TRYBB Reviewed; 218 AA.
AC Q26680;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=Flagellar calcium-binding protein TB-24;
DE AltName: Full=24 kDa calcimedin;
DE AltName: Full=24 kDa calflagin;
OS Trypanosoma brucei brucei.
OC Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC Trypanosomatida; Trypanosomatidae; Trypanosoma.
OX NCBI_TaxID=5702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=427;
RX PubMed=7818488; DOI=10.1042/bj3040833;
RA Wu Y., Deford J., Benjamin R., Lee M.G.-S., Ruben L.;
RT "The gene family of EF-hand calcium-binding proteins from the flagellum of
RT Trypanosoma brucei.";
RL Biochem. J. 304:833-841(1994).
CC -!- FUNCTION: May contribute to the rapid motility of the trypanosomes,
CC playing a role either in flagellar structure or in calcium metabolism.
CC Could alternate between a GDP-bound inactive form to a calcium/GTP-
CC bound active form.
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium, flagellum.
CC -!- DOMAIN: This protein has four EF-hand domains, three of which may be
CC functional calcium-binding sites.
CC -!- SIMILARITY: Belongs to the calflagin family. {ECO:0000305}.
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DR EMBL; U06644; AAB40004.1; -; Genomic_DNA.
DR PIR; S53354; S53354.
DR PDB; 2LVV; NMR; -; A=1-218.
DR PDBsum; 2LVV; -.
DR AlphaFoldDB; Q26680; -.
DR BMRB; Q26680; -.
DR SMR; Q26680; -.
DR SwissPalm; Q26680; -.
DR GO; GO:0031514; C:motile cilium; IEA:UniProtKB-SubCell.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR CDD; cd00051; EFh; 1.
DR DisProt; DP01669; -.
DR InterPro; IPR003299; Calflagin-bd.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR Pfam; PF13499; EF-hand_7; 1.
DR PRINTS; PR01362; CALFLAGIN.
DR SMART; SM00054; EFh; 3.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS00018; EF_HAND_1; 2.
DR PROSITE; PS50222; EF_HAND_2; 3.
PE 1: Evidence at protein level;
KW 3D-structure; Calcium; Cell projection; Cilium; Flagellum; Metal-binding;
KW Repeat.
FT CHAIN 1..218
FT /note="Flagellar calcium-binding protein TB-24"
FT /id="PRO_0000073737"
FT DOMAIN 48..83
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 84..119
FT /note="EF-hand 2"
FT /evidence="ECO:0000305"
FT DOMAIN 130..165
FT /note="EF-hand 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 167..202
FT /note="EF-hand 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT REGION 1..27
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..16
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 61
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 63
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 65
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 67
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 72
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 143
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000305"
FT BINDING 145
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000305"
FT BINDING 147
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000305"
FT BINDING 154
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000305"
FT BINDING 180
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 182
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 184
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 191
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT STRAND 20..22
FT /evidence="ECO:0007829|PDB:2LVV"
FT HELIX 27..40
FT /evidence="ECO:0007829|PDB:2LVV"
FT HELIX 47..60
FT /evidence="ECO:0007829|PDB:2LVV"
FT HELIX 61..63
FT /evidence="ECO:0007829|PDB:2LVV"
FT HELIX 71..79
FT /evidence="ECO:0007829|PDB:2LVV"
FT TURN 84..86
FT /evidence="ECO:0007829|PDB:2LVV"
FT STRAND 87..89
FT /evidence="ECO:0007829|PDB:2LVV"
FT HELIX 91..108
FT /evidence="ECO:0007829|PDB:2LVV"
FT HELIX 119..142
FT /evidence="ECO:0007829|PDB:2LVV"
FT HELIX 152..165
FT /evidence="ECO:0007829|PDB:2LVV"
FT HELIX 173..179
FT /evidence="ECO:0007829|PDB:2LVV"
FT HELIX 189..204
FT /evidence="ECO:0007829|PDB:2LVV"
FT STRAND 209..211
FT /evidence="ECO:0007829|PDB:2LVV"
SQ SEQUENCE 218 AA; 24252 MW; 041152AC2BDD6DDB CRC64;
MGCSASKDTT NSKDGAASKG GKDGKTTADR KVAWERIRCA IPRDKDAESK SRRIELFKQF
DTNGTGKLGF REVLDGCYGI LKLDEFTTHL PDIVQRAFDK AKDLGNKVKG VGEEDLVEFL
EFRLMLCYIY DIFELTVMFD TMDKDGSLLL ELQEFKEALP KLKEWGVDIT DATTVFNEID
TNGSGVVTFD EFSCWAVTKK LQVCGDPDGE ENGANEGN
