FCA4_TRYBB
ID FCA4_TRYBB Reviewed; 229 AA.
AC Q26677;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=Flagellar calcium-binding protein TB-1.7G;
DE AltName: Full=17 kDa calcimedin;
DE AltName: Full=17 kDa calflagin;
DE Flags: Fragment;
OS Trypanosoma brucei brucei.
OC Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC Trypanosomatida; Trypanosomatidae; Trypanosoma.
OX NCBI_TaxID=5702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=427;
RX PubMed=7818488; DOI=10.1042/bj3040833;
RA Wu Y., Deford J., Benjamin R., Lee M.G.-S., Ruben L.;
RT "The gene family of EF-hand calcium-binding proteins from the flagellum of
RT Trypanosoma brucei.";
RL Biochem. J. 304:833-841(1994).
CC -!- FUNCTION: May contribute to the rapid motility of the trypanosomes,
CC playing a role either in flagellar structure or in calcium metabolism.
CC Could alternate between a GDP-bound inactive form to a calcium/GTP-
CC bound active form.
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium, flagellum.
CC -!- DOMAIN: This protein has four EF-hand domains, three of which may be
CC functional calcium-binding sites.
CC -!- SIMILARITY: Belongs to the calflagin family. {ECO:0000305}.
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DR EMBL; U05882; AAA75582.1; -; Genomic_DNA.
DR PIR; S53355; S53355.
DR AlphaFoldDB; Q26677; -.
DR BMRB; Q26677; -.
DR SMR; Q26677; -.
DR GO; GO:0005929; C:cilium; IDA:GeneDB.
DR GO; GO:0005737; C:cytoplasm; IDA:GeneDB.
DR GO; GO:0031514; C:motile cilium; IEA:UniProtKB-SubCell.
DR GO; GO:0005509; F:calcium ion binding; ISM:GeneDB.
DR GO; GO:0006816; P:calcium ion transport; TAS:GeneDB.
DR CDD; cd00051; EFh; 1.
DR InterPro; IPR003299; Calflagin-bd.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR Pfam; PF13499; EF-hand_7; 1.
DR PRINTS; PR01362; CALFLAGIN.
DR SMART; SM00054; EFh; 3.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS00018; EF_HAND_1; 2.
DR PROSITE; PS50222; EF_HAND_2; 3.
PE 3: Inferred from homology;
KW Calcium; Cell projection; Cilium; Flagellum; Metal-binding; Repeat.
FT CHAIN <1..229
FT /note="Flagellar calcium-binding protein TB-1.7G"
FT /id="PRO_0000073736"
FT DOMAIN 44..79
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 80..115
FT /note="EF-hand 2"
FT /evidence="ECO:0000305"
FT DOMAIN 126..161
FT /note="EF-hand 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 163..198
FT /note="EF-hand 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 202..229
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 57
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 59
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 61
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 63
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 68
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 139
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000305"
FT BINDING 141
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000305"
FT BINDING 143
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000305"
FT BINDING 150
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000305"
FT BINDING 176
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 178
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 180
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 187
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT NON_TER 1
SQ SEQUENCE 229 AA; 25109 MW; 675A1607E390743D CRC64;
GSKNASNPKD GAASKGGKDG KTTADRKVAW ERIRCAIPRD KDAESKSRRI ELFKQFDTNG
TGKLGFREVL DGCYGILKLD EFTTHLPDIV QRAFDKAKDL GNKVKGVGEE DLVEFLEFRL
MLCYIYDIFE LTVMFDTMDK DGSLLLELQE FKEALPKLKE WGVDITDATT VFNEIDTNGS
GVVTFDEFSC WAVTKKLQVC GDPDDEENGA NEGDGANAGD GVPAAEGSA
