FCERG_MOUSE
ID FCERG_MOUSE Reviewed; 86 AA.
AC P20491;
DT 01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1991, sequence version 1.
DT 03-AUG-2022, entry version 173.
DE RecName: Full=High affinity immunoglobulin epsilon receptor subunit gamma;
DE AltName: Full=Fc receptor gamma-chain;
DE Short=FcRgamma;
DE AltName: Full=Fc-epsilon RI-gamma;
DE AltName: Full=IgE Fc receptor subunit gamma;
DE Short=FceRI gamma;
DE Flags: Precursor;
GN Name=Fcer1g {ECO:0000303|PubMed:14764707, ECO:0000303|PubMed:19098920,
GN ECO:0000312|MGI:MGI:95496}; Synonyms=Fce1g;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=2527850; DOI=10.1016/s0021-9258(19)84829-9;
RA Ra C., Jouvin M.H.E., Kinet J.-P.;
RT "Complete structure of the mouse mast cell receptor for IgE (Fc epsilon RI)
RT and surface expression of chimeric receptors (rat-mouse-human) on
RT transfected cells.";
RL J. Biol. Chem. 264:15323-15327(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Czech II; TISSUE=Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP FUNCTION IN ACTIVATION OF PLATELETS BY COLLAGEN, AND FUNCTION IN PLCG2
RP PHOSPHORYLATION.
RX PubMed=9171347; DOI=10.1093/emboj/16.9.2333;
RA Poole A., Gibbins J.M., Turner M., van Vugt M.J., van de Winkel J.G.,
RA Saito T., Tybulewicz V.L., Watson S.P.;
RT "The Fc receptor gamma-chain and the tyrosine kinase Syk are essential for
RT activation of mouse platelets by collagen.";
RL EMBO J. 16:2333-2341(1997).
RN [4]
RP FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF
RP TYR-65; TYR-76 AND 65-TYR--GLN-86.
RX PubMed=14764707; DOI=10.4049/jimmunol.172.4.2374;
RA Sakurai D., Yamasaki S., Arase K., Park S.Y., Arase H., Konno A., Saito T.;
RT "Fc epsilon RI gamma-ITAM is differentially required for mast cell function
RT in vivo.";
RL J. Immunol. 172:2374-2381(2004).
RN [5]
RP INTERACTION WITH CLEC6A.
RX PubMed=17050534; DOI=10.1074/jbc.m606542200;
RA Sato K., Yang X.L., Yudate T., Chung J.S., Wu J., Luby-Phelps K.,
RA Kimberly R.P., Underhill D., Cruz P.D. Jr., Ariizumi K.;
RT "Dectin-2 is a pattern recognition receptor for fungi that couples with the
RT Fc receptor gamma chain to induce innate immune responses.";
RL J. Biol. Chem. 281:38854-38866(2006).
RN [6]
RP FUNCTION IN INTEGRIN-MEDIATED NEUTROPHIL ACTIVATION, AND INTERACTION WITH
RP SYK.
RX PubMed=17086186; DOI=10.1038/ni1407;
RA Mocsai A., Abram C.L., Jakus Z., Hu Y., Lanier L.L., Lowell C.A.;
RT "Integrin signaling in neutrophils and macrophages uses adapters containing
RT immunoreceptor tyrosine-based activation motifs.";
RL Nat. Immunol. 7:1326-1333(2006).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-65; TYR-76 AND THR-78, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Mast cell;
RX PubMed=17947660; DOI=10.4049/jimmunol.179.9.5864;
RA Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y.,
RA Kawakami T., Salomon A.R.;
RT "Quantitative time-resolved phosphoproteomic analysis of mast cell
RT signaling.";
RL J. Immunol. 179:5864-5876(2007).
RN [8]
RP INTERACTION WITH CLEC4E.
RX PubMed=18776906; DOI=10.1038/ni.1651;
RA Yamasaki S., Ishikawa E., Sakuma M., Hara H., Ogata K., Saito T.;
RT "Mincle is an ITAM-coupled activating receptor that senses damaged cells.";
RL Nat. Immunol. 9:1179-1188(2008).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-65 AND TYR-76, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19144319; DOI=10.1016/j.immuni.2008.11.006;
RA Trost M., English L., Lemieux S., Courcelles M., Desjardins M.,
RA Thibault P.;
RT "The phagosomal proteome in interferon-gamma-activated macrophages.";
RL Immunity 30:143-154(2009).
RN [10]
RP FUNCTION, SUBUNIT, MUTAGENESIS OF ASP-29; LEU-39; TYR-65 AND TYR-76, AND
RP TISSUE SPECIFICITY.
RX PubMed=19098920; DOI=10.1038/ni.1686;
RA Hida S., Yamasaki S., Sakamoto Y., Takamoto M., Obata K., Takai T.,
RA Karasuyama H., Sugane K., Saito T., Taki S.;
RT "Fc receptor gamma-chain, a constitutive component of the IL-3 receptor, is
RT required for IL-3-induced IL-4 production in basophils.";
RL Nat. Immunol. 10:214-222(2009).
RN [11]
RP INTERACTION WITH CD300LH AND CD300LD.
RX PubMed=20817736; DOI=10.1074/jbc.m110.137166;
RA Enomoto Y., Yamanishi Y., Izawa K., Kaitani A., Takahashi M., Maehara A.,
RA Oki T., Takamatsu R., Kajikawa M., Takai T., Kitamura T., Kitaura J.;
RT "Characterization of leukocyte mono-immunoglobulin-like receptor 7
RT (LMIR7)/CLM-3 as an activating receptor: its similarities to and
RT differences from LMIR4/CLM-5.";
RL J. Biol. Chem. 285:35274-35283(2010).
RN [12]
RP FUNCTION, AND SUBUNIT.
RX PubMed=23602766; DOI=10.1016/j.immuni.2013.03.010;
RA Miyake Y., Toyonaga K., Mori D., Kakuta S., Hoshino Y., Oyamada A.,
RA Yamada H., Ono K., Suyama M., Iwakura Y., Yoshikai Y., Yamasaki S.;
RT "C-type lectin MCL is an FcRgamma-coupled receptor that mediates the
RT adjuvanticity of mycobacterial cord factor.";
RL Immunity 38:1050-1062(2013).
CC -!- FUNCTION: Adapter protein containing an immunoreceptor tyrosine-based
CC activation motif (ITAM) that transduces activation signals from various
CC immunoreceptors. As a component of the high-affinity immunoglobulin E
CC (IgE) receptor, mediates allergic inflammatory signaling in mast cells
CC (PubMed:14764707). As a constitutive component of interleukin-3
CC receptor complex, selectively mediates interleukin 4/IL4 production by
CC basophils, priming T-cells toward effector T-helper 2 subset
CC (PubMed:19098920). Associates with pattern recognition receptors CLEC4D
CC and CLEC4E to form a functional signaling complex in myeloid cells.
CC Binding of mycobacterial trehalose 6,6'-dimycolate (TDM) to this
CC receptor complex leads to phosphorylation of ITAM, triggering
CC activation of SYK, CARD9 and NF-kappa-B, consequently driving
CC maturation of antigen-presenting cells and shaping antigen-specific
CC priming of T-cells toward effector T-helper 1 and T-helper 17 cell
CC subtypes (PubMed:23602766) (Probable). May function cooperatively with
CC other activating receptors. Functionally linked to integrin beta-
CC 2/ITGB2-mediated neutrophil activation (PubMed:17086186). Also involved
CC in integrin alpha-2/ITGA2-mediated platelet activation
CC (PubMed:9171347). {ECO:0000269|PubMed:14764707,
CC ECO:0000269|PubMed:17086186, ECO:0000269|PubMed:19098920,
CC ECO:0000269|PubMed:23602766, ECO:0000269|PubMed:9171347, ECO:0000305}.
CC -!- SUBUNIT: IgE Fc receptor is a tetramer of an alpha chain, a beta chain,
CC and two disulfide linked gamma chains. Associates with FCGR1A; forms a
CC functional signaling complex (By similarity). The signaling subunit of
CC immunoglobulin gamma (IgG) Fc receptor complex. As a homodimer or a
CC heterodimer of CD247 and FCER1G, associates with the ligand binding
CC subunit FCGR3A to form a functional receptor complex (By similarity).
CC Associates with CLEC6A (PubMed:17050534). Interacts with CLEC4E
CC (PubMed:23602766, PubMed:18776906). Interacts (via ITAM domain) with
CC SYK (via SH2 domains); activates SYK, enabling integrin-mediated
CC activation of neutrophils and macrophages (PubMed:17086186). Interacts
CC with CSF2RB and recruits SYK in response to IL3 stimulation; this
CC interaction is direct (PubMed:19098920). Interacts with CD300LH; the
CC interaction may be indirect (PubMed:20817736). Interacts with CD300LD
CC (PubMed:20817736). Interacts with TARM1 (By similarity).
CC {ECO:0000250|UniProtKB:P20411, ECO:0000250|UniProtKB:P30273,
CC ECO:0000269|PubMed:17050534, ECO:0000269|PubMed:17086186,
CC ECO:0000269|PubMed:18776906, ECO:0000269|PubMed:19098920,
CC ECO:0000269|PubMed:20817736, ECO:0000269|PubMed:23602766}.
CC -!- INTERACTION:
CC P20491; Q62120: Jak2; NbExp=2; IntAct=EBI-9306159, EBI-646604;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Single-pass type I
CC membrane protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in mast cells (at protein level)
CC (PubMed:14764707). Expressed in basophils (at protein level)
CC (PubMed:19098920). {ECO:0000269|PubMed:14764707,
CC ECO:0000269|PubMed:19098920}.
CC -!- DISRUPTION PHENOTYPE: Knockout mice are resistant to IgE-mediated
CC systemic anaphylaxis. {ECO:0000269|PubMed:14764707}.
CC -!- SIMILARITY: Belongs to the CD3Z/FCER1G family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; J05020; AAA37602.1; -; mRNA.
DR EMBL; BC034163; AAH34163.1; -; mRNA.
DR CCDS; CCDS15483.1; -.
DR RefSeq; NP_034315.1; NM_010185.4.
DR AlphaFoldDB; P20491; -.
DR SMR; P20491; -.
DR BioGRID; 199616; 3.
DR IntAct; P20491; 1.
DR STRING; 10090.ENSMUSP00000078875; -.
DR iPTMnet; P20491; -.
DR PhosphoSitePlus; P20491; -.
DR SwissPalm; P20491; -.
DR jPOST; P20491; -.
DR MaxQB; P20491; -.
DR PaxDb; P20491; -.
DR PeptideAtlas; P20491; -.
DR PRIDE; P20491; -.
DR ProteomicsDB; 267364; -.
DR Antibodypedia; 20505; 168 antibodies from 29 providers.
DR DNASU; 14127; -.
DR Ensembl; ENSMUST00000079957; ENSMUSP00000078875; ENSMUSG00000058715.
DR GeneID; 14127; -.
DR KEGG; mmu:14127; -.
DR UCSC; uc007dnl.1; mouse.
DR CTD; 2207; -.
DR MGI; MGI:95496; Fcer1g.
DR VEuPathDB; HostDB:ENSMUSG00000058715; -.
DR eggNOG; ENOG502S7XC; Eukaryota.
DR GeneTree; ENSGT00390000003894; -.
DR HOGENOM; CLU_192374_0_0_1; -.
DR InParanoid; P20491; -.
DR OMA; CRLKIQM; -.
DR OrthoDB; 1577383at2759; -.
DR PhylomeDB; P20491; -.
DR TreeFam; TF330937; -.
DR Reactome; R-MMU-114604; GPVI-mediated activation cascade.
DR Reactome; R-MMU-202733; Cell surface interactions at the vascular wall.
DR Reactome; R-MMU-2454202; Fc epsilon receptor (FCERI) signaling.
DR Reactome; R-MMU-2730905; Role of LAT2/NTAL/LAB on calcium mobilization.
DR Reactome; R-MMU-2871796; FCERI mediated MAPK activation.
DR Reactome; R-MMU-2871809; FCERI mediated Ca+2 mobilization.
DR Reactome; R-MMU-2871837; FCERI mediated NF-kB activation.
DR Reactome; R-MMU-5621480; Dectin-2 family.
DR Reactome; R-MMU-6798695; Neutrophil degranulation.
DR Reactome; R-MMU-75892; Platelet Adhesion to exposed collagen.
DR BioGRID-ORCS; 14127; 2 hits in 73 CRISPR screens.
DR ChiTaRS; Fcer1g; mouse.
DR PRO; PR:P20491; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; P20491; protein.
DR Bgee; ENSMUSG00000058715; Expressed in granulocyte and 147 other tissues.
DR ExpressionAtlas; P20491; baseline and differential.
DR Genevisible; P20491; MM.
DR GO; GO:0009986; C:cell surface; IDA:MGI.
DR GO; GO:0009897; C:external side of plasma membrane; IDA:MGI.
DR GO; GO:0032998; C:Fc-epsilon receptor I complex; IMP:MGI.
DR GO; GO:0033001; C:Fc-gamma receptor III complex; ISO:MGI.
DR GO; GO:0005887; C:integral component of plasma membrane; IDA:MGI.
DR GO; GO:0045121; C:membrane raft; TAS:MGI.
DR GO; GO:0005886; C:plasma membrane; IDA:MGI.
DR GO; GO:0042802; F:identical protein binding; IPI:MGI.
DR GO; GO:0019863; F:IgE binding; IDA:MGI.
DR GO; GO:0019767; F:IgE receptor activity; IDA:MGI.
DR GO; GO:0019864; F:IgG binding; IMP:MGI.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0046982; F:protein heterodimerization activity; ISO:MGI.
DR GO; GO:0042803; F:protein homodimerization activity; ISO:MGI.
DR GO; GO:0042590; P:antigen processing and presentation of exogenous peptide antigen via MHC class I; IMP:MGI.
DR GO; GO:0019886; P:antigen processing and presentation of exogenous peptide antigen via MHC class II; IMP:MGI.
DR GO; GO:0007166; P:cell surface receptor signaling pathway; IMP:MGI.
DR GO; GO:0071404; P:cellular response to low-density lipoprotein particle stimulus; IDA:UniProtKB.
DR GO; GO:0042742; P:defense response to bacterium; IMP:MGI.
DR GO; GO:0002431; P:Fc receptor mediated stimulatory signaling pathway; IMP:MGI.
DR GO; GO:0038095; P:Fc-epsilon receptor signaling pathway; ISO:MGI.
DR GO; GO:0038094; P:Fc-gamma receptor signaling pathway; IDA:MGI.
DR GO; GO:0016064; P:immunoglobulin mediated immune response; IMP:MGI.
DR GO; GO:0045087; P:innate immune response; IMP:MGI.
DR GO; GO:0007229; P:integrin-mediated signaling pathway; IMP:UniProtKB.
DR GO; GO:0038156; P:interleukin-3-mediated signaling pathway; IMP:UniProtKB.
DR GO; GO:0045576; P:mast cell activation; IDA:MGI.
DR GO; GO:0033024; P:mast cell apoptotic process; IDA:MGI.
DR GO; GO:0043303; P:mast cell degranulation; IDA:MGI.
DR GO; GO:0033026; P:negative regulation of mast cell apoptotic process; IDA:MGI.
DR GO; GO:0002283; P:neutrophil activation involved in immune response; IMP:UniProtKB.
DR GO; GO:0030593; P:neutrophil chemotaxis; IMP:MGI.
DR GO; GO:0030316; P:osteoclast differentiation; IMP:MGI.
DR GO; GO:0006911; P:phagocytosis, engulfment; IMP:MGI.
DR GO; GO:0050778; P:positive regulation of immune response; IMP:MGI.
DR GO; GO:0032733; P:positive regulation of interleukin-10 production; IMP:MGI.
DR GO; GO:0032753; P:positive regulation of interleukin-4 production; IMP:UniProtKB.
DR GO; GO:0032755; P:positive regulation of interleukin-6 production; IDA:MGI.
DR GO; GO:0032765; P:positive regulation of mast cell cytokine production; IDA:MGI.
DR GO; GO:0043306; P:positive regulation of mast cell degranulation; IDA:MGI.
DR GO; GO:0050766; P:positive regulation of phagocytosis; IMP:MGI.
DR GO; GO:2000010; P:positive regulation of protein localization to cell surface; IDA:MGI.
DR GO; GO:0032760; P:positive regulation of tumor necrosis factor production; IDA:MGI.
DR GO; GO:0001812; P:positive regulation of type I hypersensitivity; IMP:MGI.
DR GO; GO:0001798; P:positive regulation of type IIa hypersensitivity; IMP:MGI.
DR GO; GO:0001805; P:positive regulation of type III hypersensitivity; IMP:MGI.
DR GO; GO:0072659; P:protein localization to plasma membrane; IDA:MGI.
DR GO; GO:0031623; P:receptor internalization; IDA:UniProtKB.
DR GO; GO:0050776; P:regulation of immune response; IMP:MGI.
DR GO; GO:0010543; P:regulation of platelet activation; IMP:UniProtKB.
DR GO; GO:0002554; P:serotonin secretion by platelet; IMP:UniProtKB.
DR GO; GO:0007165; P:signal transduction; IDA:MGI.
DR GO; GO:0002292; P:T cell differentiation involved in immune response; IMP:MGI.
DR InterPro; IPR021663; CD3_zeta/IgE_Fc_rcpt_gamma.
DR InterPro; IPR042340; FCER1G.
DR InterPro; IPR003110; Phos_immunorcpt_sig_ITAM.
DR PANTHER; PTHR16803; PTHR16803; 1.
DR Pfam; PF02189; ITAM; 1.
DR Pfam; PF11628; TCR_zetazeta; 1.
DR SMART; SM00077; ITAM; 1.
DR PROSITE; PS51055; ITAM_1; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Disulfide bond; IgE-binding protein; Immunity;
KW Innate immunity; Membrane; Metal-binding; Phosphoprotein; Receptor;
KW Reference proteome; Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..18
FT /evidence="ECO:0000255"
FT CHAIN 19..86
FT /note="High affinity immunoglobulin epsilon receptor
FT subunit gamma"
FT /id="PRO_0000016503"
FT TOPO_DOM 19..23
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 24..44
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 45..86
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 54..82
FT /note="ITAM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00379"
FT MOD_RES 65
FT /note="Phosphotyrosine"
FT /evidence="ECO:0007744|PubMed:17947660,
FT ECO:0007744|PubMed:19144319"
FT MOD_RES 76
FT /note="Phosphotyrosine"
FT /evidence="ECO:0007744|PubMed:17947660,
FT ECO:0007744|PubMed:19144319"
FT MOD_RES 78
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:17947660"
FT DISULFID 25
FT /note="Interchain"
FT /evidence="ECO:0000250"
FT MUTAGEN 29
FT /note="D->A: Increases IL3-induced production of IL4 by
FT basophils."
FT /evidence="ECO:0000269|PubMed:19098920"
FT MUTAGEN 39
FT /note="L->A: Impairs interaction with CSF2RB. Impairs IL3-
FT induced production of IL4 by basophils."
FT /evidence="ECO:0000269|PubMed:19098920"
FT MUTAGEN 65..86
FT /note="Missing: Impairs IgE-induced mast cell activation in
FT the presence of antigen; Impairs IgE-induced mast cell
FT survival in the absence of antigen."
FT /evidence="ECO:0000269|PubMed:14764707"
FT MUTAGEN 65
FT /note="Y->P: Impairs IgE-induced mast cell activation in
FT the presence of antigen; Impairs IgE-induced mast cell
FT survival in the absence of antigen; when associated with P-
FT 76. Impairs IL3-induced production of IL4 by basophils;
FT when associated with P-76."
FT /evidence="ECO:0000269|PubMed:14764707,
FT ECO:0000269|PubMed:19098920"
FT MUTAGEN 76
FT /note="Y->P: Impairs IgE-induced mast cell activation in
FT the presence of antigen; Impairs IgE-induced mast cell
FT survival in the absence of antigen; when associated with P-
FT 65. Impairs IL3-induced production of IL4 by basophils;
FT when associated with P-65."
FT /evidence="ECO:0000269|PubMed:14764707,
FT ECO:0000269|PubMed:19098920"
SQ SEQUENCE 86 AA; 9652 MW; 83184DE22FCC9ECB CRC64;
MISAVILFLL LLVEQAAALG EPQLCYILDA VLFLYGIVLT LLYCRLKIQV RKAAIASREK
ADAVYTGLNT RSQETYETLK HEKPPQ
