FCF1_SCHPO
ID FCF1_SCHPO Reviewed; 192 AA.
AC O13610;
DT 10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 25-MAY-2022, entry version 122.
DE RecName: Full=rRNA-processing protein fcf1;
GN Name=fcf1; ORFNames=SPBC32H8.04c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=10620777;
RX DOI=10.1002/(sici)1097-0061(20000115)16:1<71::aid-yea505>3.0.co;2-5;
RA Machida M., Yamazaki S., Kunihiro S., Tanaka T., Kushida N., Jinno K.,
RA Haikawa Y., Yamazaki J., Yamamoto S., Sekine M., Oguchi A., Nagai Y.,
RA Sakai M., Aoki K., Ogura K., Kudoh Y., Kikuchi H., Zhang M.Q., Yanagida M.;
RT "A 38 kb segment containing the cdc2 gene from the left arm of fission
RT yeast chromosome II: sequence analysis and characterization of the genomic
RT DNA and cDNAs encoded on the segment.";
RL Yeast 16:71-80(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [3]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
CC -!- FUNCTION: Essential protein involved in pre-rRNA processing and 40S
CC ribosomal subunit assembly. Required for the early cleavage steps of
CC 35S rRNA at the A(0), A(1), and A(2) sites (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:16823372}.
CC -!- SIMILARITY: Belongs to the UTP23/FCF1 family. FCF1 subfamily.
CC {ECO:0000305}.
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DR EMBL; AB004535; BAA21398.1; -; Genomic_DNA.
DR EMBL; CU329671; CAC37494.1; -; Genomic_DNA.
DR RefSeq; NP_595610.1; NM_001021505.2.
DR PDB; 5YZ4; X-ray; 2.13 A; A=60-192.
DR PDBsum; 5YZ4; -.
DR AlphaFoldDB; O13610; -.
DR SMR; O13610; -.
DR BioGRID; 276751; 2.
DR STRING; 4896.SPBC32H8.04c.1; -.
DR MaxQB; O13610; -.
DR PaxDb; O13610; -.
DR EnsemblFungi; SPBC32H8.04c.1; SPBC32H8.04c.1:pep; SPBC32H8.04c.
DR PomBase; SPBC32H8.04c; fcf1.
DR VEuPathDB; FungiDB:SPBC32H8.04c; -.
DR eggNOG; KOG3165; Eukaryota.
DR HOGENOM; CLU_081098_0_1_1; -.
DR InParanoid; O13610; -.
DR OMA; PLITDCV; -.
DR PhylomeDB; O13610; -.
DR Reactome; R-SPO-6791226; Major pathway of rRNA processing in the nucleolus and cytosol.
DR PRO; PR:O13610; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0005730; C:nucleolus; HDA:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0032040; C:small-subunit processome; ISO:PomBase.
DR GO; GO:0004521; F:endoribonuclease activity; IMP:PomBase.
DR GO; GO:0000447; P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:PomBase.
DR GO; GO:0000472; P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:PomBase.
DR CDD; cd09864; PIN_Fcf1-like; 1.
DR InterPro; IPR006984; Fcf1/Utp23.
DR InterPro; IPR037503; Fcf1_PIN.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR002716; PIN_dom.
DR Pfam; PF04900; Fcf1; 1.
DR SMART; SM00670; PINc; 1.
DR SUPFAM; SSF88723; SSF88723; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Nucleus; Reference proteome; Ribosome biogenesis;
KW rRNA processing.
FT CHAIN 1..192
FT /note="rRNA-processing protein fcf1"
FT /id="PRO_0000339875"
FT DOMAIN 65..164
FT /note="PINc"
FT STRAND 63..69
FT /evidence="ECO:0007829|PDB:5YZ4"
FT HELIX 73..79
FT /evidence="ECO:0007829|PDB:5YZ4"
FT HELIX 84..92
FT /evidence="ECO:0007829|PDB:5YZ4"
FT STRAND 96..101
FT /evidence="ECO:0007829|PDB:5YZ4"
FT HELIX 102..110
FT /evidence="ECO:0007829|PDB:5YZ4"
FT HELIX 112..115
FT /evidence="ECO:0007829|PDB:5YZ4"
FT HELIX 116..122
FT /evidence="ECO:0007829|PDB:5YZ4"
FT STRAND 128..130
FT /evidence="ECO:0007829|PDB:5YZ4"
FT HELIX 139..149
FT /evidence="ECO:0007829|PDB:5YZ4"
FT STRAND 154..156
FT /evidence="ECO:0007829|PDB:5YZ4"
FT HELIX 160..166
FT /evidence="ECO:0007829|PDB:5YZ4"
FT STRAND 174..178
FT /evidence="ECO:0007829|PDB:5YZ4"
FT STRAND 181..188
FT /evidence="ECO:0007829|PDB:5YZ4"
SQ SEQUENCE 192 AA; 22352 MW; AD1F8F0D782FCD31 CRC64;
MGKAKTTRKF AQVKRVINLK DQRLQKKDQK KEKEKTTKNG ELVREIPQMA SNLFFQFNES
LGPPYHVIID TNFINFCLQQ KIDLFEGLMT CLYAKTIPCI SDCVMAELEK LGIRYRIALR
IAKDERFERL PCTHKGTYAD DCIVQRVMQH KCYLVATNDK NLKQRIRKIP GIPILSVANH
KIRVERLVDV VD
