FCF1_YEAST
ID FCF1_YEAST Reviewed; 189 AA.
AC Q05498; D6VSX1;
DT 17-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=rRNA-processing protein FCF1;
DE AltName: Full=FAF1-copurifying factor 1;
GN Name=FCF1; OrderedLocusNames=YDR339C; ORFNames=D9651.9;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH FAF1.
RX PubMed=16762320; DOI=10.1016/j.bbrc.2006.05.140;
RA Rempola B., Karkusiewicz I., Piekarska I., Rytka J.;
RT "Fcf1p and Fcf2p are novel nucleolar Saccharomyces cerevisiae proteins
RT involved in pre-rRNA processing.";
RL Biochem. Biophys. Res. Commun. 346:546-554(2006).
CC -!- FUNCTION: Essential protein involved in pre-rRNA processing and 40S
CC ribosomal subunit assembly. Required for the early cleavage steps of
CC 35S rRNA at the A(0), A(1), and A(2) sites.
CC {ECO:0000269|PubMed:16762320}.
CC -!- SUBUNIT: Interacts with FAF1. {ECO:0000269|PubMed:16762320}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:16762320}.
CC -!- SIMILARITY: Belongs to the UTP23/FCF1 family. FCF1 subfamily.
CC {ECO:0000305}.
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DR EMBL; U51032; AAB64775.1; -; Genomic_DNA.
DR EMBL; AY557728; AAS56054.1; -; Genomic_DNA.
DR EMBL; BK006938; DAA12181.1; -; Genomic_DNA.
DR PIR; S70104; S70104.
DR RefSeq; NP_010626.1; NM_001180647.1.
DR PDB; 5WLC; EM; 3.80 A; SL=1-189.
DR PDB; 5WYJ; EM; 8.70 A; U4=1-189.
DR PDB; 5WYK; EM; 4.50 A; U4=1-189.
DR PDB; 6KE6; EM; 3.40 A; 5K=1-189.
DR PDB; 6LQP; EM; 3.20 A; 5K=1-189.
DR PDB; 6LQQ; EM; 4.10 A; 5K=1-189.
DR PDB; 6LQR; EM; 8.60 A; 5K=1-189.
DR PDB; 6LQS; EM; 3.80 A; 5K=1-189.
DR PDB; 6LQT; EM; 4.90 A; 5K=1-189.
DR PDB; 6LQU; EM; 3.70 A; 5K=1-189.
DR PDB; 6LQV; EM; 4.80 A; 5K=1-189.
DR PDB; 6ND4; EM; 4.30 A; l=1-189.
DR PDB; 6ZQA; EM; 4.40 A; UX=1-189.
DR PDB; 6ZQB; EM; 3.90 A; UX=1-189.
DR PDB; 6ZQC; EM; 3.80 A; UX=1-189.
DR PDB; 6ZQD; EM; 3.80 A; UX=1-189.
DR PDB; 6ZQE; EM; 7.10 A; UX=1-189.
DR PDB; 6ZQG; EM; 3.50 A; UX=1-189.
DR PDB; 7AJT; EM; 4.60 A; UX=1-189.
DR PDB; 7AJU; EM; 3.80 A; UX=1-189.
DR PDB; 7D4I; EM; 4.00 A; 5K=1-189.
DR PDB; 7D5S; EM; 4.60 A; 5K=1-189.
DR PDB; 7D5T; EM; 6.00 A; 5K=1-189.
DR PDB; 7D63; EM; 12.30 A; 5K=1-189.
DR PDBsum; 5WLC; -.
DR PDBsum; 5WYJ; -.
DR PDBsum; 5WYK; -.
DR PDBsum; 6KE6; -.
DR PDBsum; 6LQP; -.
DR PDBsum; 6LQQ; -.
DR PDBsum; 6LQR; -.
DR PDBsum; 6LQS; -.
DR PDBsum; 6LQT; -.
DR PDBsum; 6LQU; -.
DR PDBsum; 6LQV; -.
DR PDBsum; 6ND4; -.
DR PDBsum; 6ZQA; -.
DR PDBsum; 6ZQB; -.
DR PDBsum; 6ZQC; -.
DR PDBsum; 6ZQD; -.
DR PDBsum; 6ZQE; -.
DR PDBsum; 6ZQG; -.
DR PDBsum; 7AJT; -.
DR PDBsum; 7AJU; -.
DR PDBsum; 7D4I; -.
DR PDBsum; 7D5S; -.
DR PDBsum; 7D5T; -.
DR PDBsum; 7D63; -.
DR AlphaFoldDB; Q05498; -.
DR SMR; Q05498; -.
DR BioGRID; 32396; 264.
DR ComplexPortal; CPX-1604; Small ribosomal subunit processome, variant 1.
DR ComplexPortal; CPX-1607; Small ribosomal subunit processome, variant 2.
DR ComplexPortal; CPX-1608; Small ribosomal subunit processome, variant 3.
DR DIP; DIP-6627N; -.
DR IntAct; Q05498; 23.
DR MINT; Q05498; -.
DR STRING; 4932.YDR339C; -.
DR iPTMnet; Q05498; -.
DR MaxQB; Q05498; -.
DR PaxDb; Q05498; -.
DR PRIDE; Q05498; -.
DR TopDownProteomics; Q05498; -.
DR EnsemblFungi; YDR339C_mRNA; YDR339C; YDR339C.
DR GeneID; 851939; -.
DR KEGG; sce:YDR339C; -.
DR SGD; S000002747; FCF1.
DR VEuPathDB; FungiDB:YDR339C; -.
DR eggNOG; KOG3165; Eukaryota.
DR GeneTree; ENSGT00940000153117; -.
DR HOGENOM; CLU_081098_0_1_1; -.
DR InParanoid; Q05498; -.
DR OMA; PLITDCV; -.
DR BioCyc; YEAST:G3O-29895-MON; -.
DR Reactome; R-SCE-6791226; Major pathway of rRNA processing in the nucleolus and cytosol.
DR PRO; PR:Q05498; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; Q05498; protein.
DR GO; GO:0005730; C:nucleolus; IDA:SGD.
DR GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR GO; GO:0032040; C:small-subunit processome; IDA:SGD.
DR GO; GO:0004521; F:endoribonuclease activity; IDA:SGD.
DR GO; GO:0000480; P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD.
DR GO; GO:0000447; P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD.
DR GO; GO:0000472; P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD.
DR GO; GO:0030490; P:maturation of SSU-rRNA; IC:ComplexPortal.
DR CDD; cd09864; PIN_Fcf1-like; 1.
DR InterPro; IPR006984; Fcf1/Utp23.
DR InterPro; IPR037503; Fcf1_PIN.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR002716; PIN_dom.
DR Pfam; PF04900; Fcf1; 1.
DR SMART; SM00670; PINc; 1.
DR SUPFAM; SSF88723; SSF88723; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Nucleus; Reference proteome; Ribosome biogenesis;
KW rRNA processing.
FT CHAIN 1..189
FT /note="rRNA-processing protein FCF1"
FT /id="PRO_0000253818"
FT DOMAIN 63..162
FT /note="PINc"
SQ SEQUENCE 189 AA; 21611 MW; 0089AD246BA08473 CRC64;
MGKAKKTRKF GLVKRTLNTK KDQRLKKNQE NIKTKEDPEL TRNIPQVSSA LFFQYNQAIK
PPYQVLIDTN FINFSIQKKV DIVRGMMDCL LAKCNPLITD CVMAELEKLG PKYRIALKLA
RDPRIKRLSC SHKGTYADDC LVHRVLQHKC YIVATNDAGL KQRIRKIPGI PLMSVGGHAY
VIEKLPDVF
