ID   FCHA_METEA              Reviewed;         208 AA.
AC   Q49135; C5B109;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   22-SEP-2009, sequence version 2.
DT   03-AUG-2022, entry version 117.
DE   RecName: Full=Methenyltetrahydrofolate cyclohydrolase;
DE            EC=3.5.4.9;
GN   Name=fchA; OrderedLocusNames=MexAM1_META1p1729;
OS   Methylorubrum extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB
OS   9133 / AM1) (Methylobacterium extorquens).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Methylobacteriaceae; Methylorubrum.
OX   NCBI_TaxID=272630;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DISRUPTION PHENOTYPE.
RX   PubMed=7961516; DOI=10.1128/jb.176.23.7398-7404.1994;
RA   Chistoserdova L.V., Lidstrom M.E.;
RT   "Genetics of the serine cycle in Methylobacterium extorquens AM1:
RT   identification, sequence, and mutation of three new genes involved in C1
RT   assimilation, orf4, mtkA, and mtkB.";
RL   J. Bacteriol. 176:7398-7404(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1;
RX   PubMed=19440302; DOI=10.1371/journal.pone.0005584;
RA   Vuilleumier S., Chistoserdova L., Lee M.-C., Bringel F., Lajus A., Zhou Y.,
RA   Gourion B., Barbe V., Chang J., Cruveiller S., Dossat C., Gillett W.,
RA   Gruffaz C., Haugen E., Hourcade E., Levy R., Mangenot S., Muller E.,
RA   Nadalig T., Pagni M., Penny C., Peyraud R., Robinson D.G., Roche D.,
RA   Rouy Z., Saenampechek C., Salvignol G., Vallenet D., Wu Z., Marx C.J.,
RA   Vorholt J.A., Olson M.V., Kaul R., Weissenbach J., Medigue C.,
RA   Lidstrom M.E.;
RT   "Methylobacterium genome sequences: a reference blueprint to investigate
RT   microbial metabolism of C1 compounds from natural and industrial sources.";
RL   PLoS ONE 4:E5584-E5584(2009).
RN   [3]
RP   CHARACTERIZATION, AND PROTEIN SEQUENCE OF 1-21.
RX   PubMed=10215859; DOI=10.1046/j.1432-1327.1999.00291.x;
RA   Pomper B.K., Vorholt J.A., Chistoserdova L.V., Lidstrom M.E., Thauer R.K.;
RT   "A methenyl tetrahydromethanopterin cyclohydrolase and a methenyl
RT   tetrahydrofolate cyclohydrolase in Methylobacterium extorquens AM1.";
RL   Eur. J. Biochem. 261:475-480(1999).
CC   -!- FUNCTION: Required for both C1 and C2 metabolism.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5,10-methenyltetrahydrofolate + H2O = (6S)-10-
CC         formyltetrahydrofolate + H(+); Xref=Rhea:RHEA:23700,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57454,
CC         ChEBI:CHEBI:57455; EC=3.5.4.9;
CC   -!- PATHWAY: One-carbon metabolism; formaldehyde assimilation via serine
CC       pathway.
CC   -!- SUBUNIT: Homodimer.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- DISRUPTION PHENOTYPE: Mutants are unable to grow on either methanol or
CC       ethylamine in the presence of glycolate. Glyoxylate restores growth,
CC       but only on C2 compounds. {ECO:0000269|PubMed:7961516}.
CC   -!- SIMILARITY: Belongs to the cyclodeaminase/cyclohydrolase family.
CC       {ECO:0000305}.
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DR   EMBL; L33465; AAA62653.1; -; Genomic_DNA.
DR   EMBL; CP001510; ACS39573.1; -; Genomic_DNA.
DR   PIR; A55230; A55230.
DR   RefSeq; WP_003597635.1; NC_012988.1.
DR   AlphaFoldDB; Q49135; -.
DR   SMR; Q49135; -.
DR   STRING; 272630.MexAM1_META1p1729; -.
DR   EnsemblBacteria; ACS39573; ACS39573; MexAM1_META1p1729.
DR   KEGG; mea:Mex_1p1729; -.
DR   eggNOG; COG3404; Bacteria.
DR   HOGENOM; CLU_088419_0_1_5; -.
DR   OMA; VAMVARY; -.
DR   OrthoDB; 1965647at2; -.
DR   BioCyc; MetaCyc:MON-3943; -.
DR   UniPathway; UPA00927; -.
DR   Proteomes; UP000009081; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0004477; F:methenyltetrahydrofolate cyclohydrolase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.120.680; -; 1.
DR   InterPro; IPR007044; Cyclodeamin/CycHdrlase.
DR   InterPro; IPR036178; Formintransfe-cycloase-like_sf.
DR   Pfam; PF04961; FTCD_C; 1.
DR   SUPFAM; SSF101262; SSF101262; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Hydrolase; Membrane; One-carbon metabolism;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..208
FT                   /note="Methenyltetrahydrofolate cyclohydrolase"
FT                   /id="PRO_0000087208"
FT   TRANSMEM        25..46
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        59
FT                   /note="K -> M (in Ref. 1; AAA62653)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   208 AA;  21723 MW;  A40F612E8CB9BC19 CRC64;
     MAGNETIETF LDGLASSAPT PGGGGAAAIS GAMGAALVSM VCNLTIGKKK YVEVEADLKQ
     VLEKSEGLRR TLTGMIADDV EAFDAVMGAY GLPKNTDEEK AARAAKIQEA LKTATDVPLA
     CCRVCREVID LAEIVAEKGN LNVISDAGVA VLSAYAGLRS AALNVYVNAK GLDDRAFAEE
     RLKELEGLLA EAGALNERIY ETVKSKVN