ID   FCK4_FUSPC              Reviewed;         490 AA.
AC   P0DPA5;
DT   25-OCT-2017, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2017, sequence version 1.
DT   25-MAY-2022, entry version 9.
DE   RecName: Full=Probable alcohol acetyltransferase FCK4 {ECO:0000303|PubMed:28802024};
DE            EC=2.3.1.- {ECO:0000305|PubMed:28802024};
DE   AltName: Full=Cytokinin biosynthesis protein 4 {ECO:0000303|PubMed:28802024};
GN   Name=FCK4 {ECO:0000303|PubMed:28802024};
GN   ORFNames=FPSE_20002 {ECO:0000303|PubMed:28802024};
OS   Fusarium pseudograminearum (strain CS3096) (Wheat and barley crown-rot
OS   fungus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium.
OX   NCBI_TaxID=1028729;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CS3096;
RX   PubMed=23028337; DOI=10.1371/journal.ppat.1002952;
RA   Gardiner D.M., McDonald M.C., Covarelli L., Solomon P.S., Rusu A.G.,
RA   Marshall M., Kazan K., Chakraborty S., McDonald B.A., Manners J.M.;
RT   "Comparative pathogenomics reveals horizontally acquired novel virulence
RT   genes in fungi infecting cereal hosts.";
RL   PLoS Pathog. 8:E1002952-E1002952(2012).
RN   [2]
RP   GENOME REANNOTATION, FUNCTION, DISRUPTION PHENOTYPE, INDUCTION, AND
RP   PATHWAY.
RC   STRAIN=CS3096;
RX   PubMed=28802024; DOI=10.1111/mpp.12593;
RA   Soerensen J.L., Benfield A.H., Wollenberg R.D., Westphal K., Wimmer R.,
RA   Nielsen M.R., Nielsen K.F., Carere J., Covarelli L., Beccari G., Powell J.,
RA   Yamashino T., Kogler H., Sondergaard T.E., Gardiner D.M.;
RT   "The cereal pathogen Fusarium pseudograminearum produces a new class of
RT   active cytokinins during infection.";
RL   Mol. Plant Pathol. 19:1140-1154(2018).
CC   -!- FUNCTION: Probable alcohol acetyltransferase; part of the gene cluster
CC       that mediates the biosynthesis of cytokinins such as fusatin, fusatinic
CC       acids or 8-oxofusatin, known for their growth promoting and anti-
CC       senescence activities toward host plants (PubMed:28802024). FCK1 is a
CC       bifunctional enzyme that performs the first steps in the biosynthesis
CC       of Fusarium cytokinins (PubMed:28802024). It first condenses adenosine
CC       monophosphate (AMP) with dimethylallyl diphosphate (DMAPP) to yield
CC       isoprenyl adenosine monophosphate (PubMed:28802024). It then catalyzes
CC       the removal of the phosphoribose to produce isopentenylaldehyde
CC       (PubMed:28802024). The cytochrome P450 monooxygenase then converts
CC       isopentenylaldehyde to trans-zeatin (PubMed:28802024). A condensation
CC       step converts trans-zeatin to fusatin which is further modified to
CC       produce fusatinic acid (PubMed:28802024). The mechanism for oxidation
CC       of fusatin to fusatinic acid remains unknown (PubMed:28802024). 8-
CC       oxofusatin could be produced through several pathways, via direct
CC       oxygenation of fusatin, or via the 8-oxo-pentenyladenine intermediate
CC       which itself must arise from either the prenylation of 8-oxo-AMP by
CC       FCK1 and/or oxygenation of isopentenylaldehyde (PubMed:28802024). Both
CC       the FCK3 and FCK4 enzymes act downstream of the identified cytokinins
CC       to produce yet unidentified compounds (PubMed:28802024).
CC   -!- PATHWAY: Secondary metabolite biosynthesis.
CC       {ECO:0000269|PubMed:28802024}.
CC   -!- INDUCTION: Expressed during infection of barley and Brachypodium
CC       (PubMed:28802024). {ECO:0000269|PubMed:28802024}.
CC   -!- DISRUPTION PHENOTYPE: Results in enhanced production of cytokinins
CC       (PubMed:28802024). {ECO:0000269|PubMed:28802024}.
CC   -!- SIMILARITY: Belongs to the alcohol acetyltransferase FCK4 family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AFNW01000149; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   AlphaFoldDB; P0DPA5; -.
DR   SMR; P0DPA5; -.
DR   Proteomes; UP000007978; Chromosome 3.
DR   Proteomes; UP000007978; Unassembled WGS sequence.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.559.10; -; 1.
DR   InterPro; IPR010828; Atf2/Sli1-like.
DR   InterPro; IPR023213; CAT-like_dom_sf.
DR   Pfam; PF07247; AATase; 1.
PE   2: Evidence at transcript level;
KW   Transferase.
FT   CHAIN           1..490
FT                   /note="Probable alcohol acetyltransferase FCK4"
FT                   /id="PRO_0000442156"
SQ   SEQUENCE   490 AA;  54751 MW;  0ED7FE382F913A42 CRC64;
     MTDSIHGGHP NIIRSCGASE SMWTASHALG LYLRVSNIAT LTIPTWKAKF KKSSASHFIR
     TAVVQVMLKQ PAMRVGIIGE NSKQPRFVAL SSVDFSQQVE YAEAPSVDSV ARSLEKLLLQ
     PWPNLSKRPG WHVRVFHEPS SADKDVCRLR ICLTVHHAIF DGESTARFHT SLIDALNNPD
     PAVARMMDKS PVVNLTGDCR DYPLTQEELI NFTADIFWIA SELWNILAPS FLKPFTFQTW
     AGEPYNPDIQ AINIRYLSLN PIVTQAVVKR CREEKTTMTN LLNTLCATSL ARRVPLTERQ
     GFVNLTAVSL RPWIPENLHP NKTCMSVCAS SHAQDFGPDV LAKIRQAEDA TIWELAVKLR
     QDMKRRTDTM PHNEFSYLLR YAGDWRDIFQ ARYGKPRREL WTLSNLGSIA TPASKGPWEL
     ESVLFAQFAP VVSCALAVNA ASVEGGDMMV SLVWQDGILE TDLVEGVRDD LEMWLLGLGN
     EGNLGISSSM