FCL1_ORYSJ
ID FCL1_ORYSJ Reviewed; 328 AA.
AC Q67WR2; Q0DAH4;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Probable GDP-L-fucose synthase 1;
DE EC=1.1.1.271 {ECO:0000250|UniProtKB:O49213};
DE AltName: Full=GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase 1;
GN OrderedLocusNames=Os06g0652400, LOC_Os06g44270;
GN ORFNames=OsJ_22191 {ECO:0000312|EMBL:EAZ37847.1}, OSJNBa0085J13.7;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- FUNCTION: Catalyzes the two-step NADP-dependent conversion of GDP-4-
CC dehydro-6-deoxy-D-mannose to GDP-fucose, involving an epimerase and a
CC reductase reaction. {ECO:0000250|UniProtKB:O49213}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=GDP-beta-L-fucose + NADP(+) = GDP-4-dehydro-alpha-D-rhamnose +
CC H(+) + NADPH; Xref=Rhea:RHEA:18885, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57273, ChEBI:CHEBI:57783, ChEBI:CHEBI:57964,
CC ChEBI:CHEBI:58349; EC=1.1.1.271;
CC Evidence={ECO:0000250|UniProtKB:O49213};
CC -!- PATHWAY: Nucleotide-sugar biosynthesis; GDP-L-fucose biosynthesis via
CC de novo pathway; GDP-L-fucose from GDP-alpha-D-mannose: step 2/2.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the NAD(P)-dependent epimerase/dehydratase
CC family. Fucose synthase subfamily. {ECO:0000305}.
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DR EMBL; AP003565; BAD37407.1; -; Genomic_DNA.
DR EMBL; AP008212; BAF20149.1; -; Genomic_DNA.
DR EMBL; AP014962; BAS98916.1; -; Genomic_DNA.
DR EMBL; CM000143; EAZ37847.1; -; Genomic_DNA.
DR EMBL; AK071960; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AK103529; BAG96133.1; -; mRNA.
DR EMBL; AK120997; BAH00264.1; -; mRNA.
DR RefSeq; XP_015644414.1; XM_015788928.1.
DR AlphaFoldDB; Q67WR2; -.
DR SMR; Q67WR2; -.
DR BioGRID; 810736; 1.
DR STRING; 4530.OS06T0652400-02; -.
DR PaxDb; Q67WR2; -.
DR PRIDE; Q67WR2; -.
DR EnsemblPlants; Os06t0652400-01; Os06t0652400-01; Os06g0652400.
DR EnsemblPlants; Os06t0652400-02; Os06t0652400-02; Os06g0652400.
DR GeneID; 4341699; -.
DR Gramene; Os06t0652400-01; Os06t0652400-01; Os06g0652400.
DR Gramene; Os06t0652400-02; Os06t0652400-02; Os06g0652400.
DR KEGG; osa:4341699; -.
DR eggNOG; KOG1431; Eukaryota.
DR HOGENOM; CLU_007383_18_0_1; -.
DR InParanoid; Q67WR2; -.
DR OMA; IHCAGRV; -.
DR OrthoDB; 922791at2759; -.
DR PlantReactome; R-OSA-1119620; GDP-L-fucose biosynthesis I (from GDP-D-mannose).
DR UniPathway; UPA00128; UER00191.
DR Proteomes; UP000000763; Chromosome 6.
DR Proteomes; UP000007752; Chromosome 6.
DR Proteomes; UP000059680; Chromosome 6.
DR Genevisible; Q67WR2; OS.
DR GO; GO:0050577; F:GDP-L-fucose synthase activity; IBA:GO_Central.
DR GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR GO; GO:0042351; P:'de novo' GDP-L-fucose biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd05239; GDP_FS_SDR_e; 1.
DR HAMAP; MF_00956; GDP_fucose_synth; 1.
DR InterPro; IPR001509; Epimerase_deHydtase.
DR InterPro; IPR028614; GDP_fucose/colitose_synth.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR Pfam; PF01370; Epimerase; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
PE 2: Evidence at transcript level;
KW Isomerase; Multifunctional enzyme; NADP; Oxidoreductase;
KW Reference proteome.
FT CHAIN 1..328
FT /note="Probable GDP-L-fucose synthase 1"
FT /id="PRO_0000174356"
FT ACT_SITE 152
FT /note="Proton donor/acceptor"
FT /evidence="ECO:0000250"
FT BINDING 25..31
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250"
FT BINDING 156
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250"
FT BINDING 179..182
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250"
FT BINDING 195
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250"
FT BINDING 203
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 218
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 225
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 285
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT SITE 123
FT /note="Important for catalytic activity"
FT /evidence="ECO:0000250"
FT SITE 125
FT /note="Important for catalytic activity"
FT /evidence="ECO:0000250"
FT SITE 156
FT /note="Lowers pKa of active site Tyr"
FT /evidence="ECO:0000250"
FT CONFLICT 28
FT /note="G -> S (in Ref. 5; AK071960)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 328 AA; 35715 MW; 87D1D0335D807119 CRC64;
MGTVTTADPH ASFLADKGGK VFVAGHRGLV GSAILRHLVS LGFTNVVVRT HAELDLTRQS
DVEAFFAAEL PRYVVLAAAK VGGIHANSTF PADFIAANLQ IQTNVVDAAL KCGSVRKLLF
LGSSCIYPKF APQPIPENSL LSGPLEPTNE WYAVAKIAGI KMCQAYRIQH GFDAISAMPT
NLYGPQDNFH PENSHVLPAL IRRFHEAKAS NAAEVVVWGT GSPLREFLHV DDLADAVIFL
MDHYSGLEHV NVGSGSEVTI KELAELVKEV VGFQGKLVWD SSKPDGTPRK LMDSSKIQEM
GWKPKVPLKE GLVETYKWYV ENVISAKK
