FCSB_DICDI
ID FCSB_DICDI Reviewed; 673 AA.
AC Q1ZXQ4;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 02-MAY-2006, sequence version 1.
DT 25-MAY-2022, entry version 88.
DE RecName: Full=Fatty acyl-CoA synthetase B;
DE EC=6.2.1.3;
DE AltName: Full=Long chain fatty acyl coenzyme A-synthetase 2;
DE Short=LC-FACS 2;
DE AltName: Full=Long-chain-fatty-acid--CoA synthetase 2;
DE Flags: Precursor;
GN Name=fcsB; ORFNames=DDB_G0269474;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [2]
RP SUBCELLULAR LOCATION.
RX PubMed=14629118; DOI=10.1078/0171-9335-00342;
RA von Loehneysen K., Pawolleck N., Ruehling H., Maniak M.;
RT "A Dictyostelium long chain fatty acyl coenzyme A-synthetase mediates fatty
RT acid retrieval from endosomes.";
RL Eur. J. Cell Biol. 82:505-514(2003).
RN [3]
RP INDUCTION [LARGE SCALE ANALYSIS].
RX PubMed=18590548; DOI=10.1186/1471-2180-8-109;
RA Carilla-Latorre S., Calvo-Garrido J., Bloomfield G., Skelton J., Kay R.R.,
RA Ivens A., Martinez J.L., Escalante R.;
RT "Dictyostelium transcriptional responses to Pseudomonas aeruginosa: common
RT and specific effects from PAO1 and PA14 strains.";
RL BMC Microbiol. 8:109-109(2008).
CC -!- FUNCTION: Long chain fatty acid acyl-CoA synthetases catalyze the
CC formation of a thiester bond between a free fatty acid and coenzyme A
CC during fatty acid metabolic process. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a long-chain fatty acid + ATP + CoA = a long-chain fatty acyl-
CC CoA + AMP + diphosphate; Xref=Rhea:RHEA:15421, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:57287, ChEBI:CHEBI:57560,
CC ChEBI:CHEBI:83139, ChEBI:CHEBI:456215; EC=6.2.1.3;
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum
CC {ECO:0000269|PubMed:14629118}.
CC -!- INDUCTION: Down-regulated by Pseudomonas aeruginosa, PAO1 strain and
CC PA14 strain infection. {ECO:0000269|PubMed:18590548}.
CC -!- SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family.
CC {ECO:0000305}.
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DR EMBL; AAFI02000005; EAS66934.1; -; Genomic_DNA.
DR RefSeq; XP_001134470.1; XM_001134470.1.
DR AlphaFoldDB; Q1ZXQ4; -.
DR SMR; Q1ZXQ4; -.
DR STRING; 44689.DDB0231574; -.
DR PaxDb; Q1ZXQ4; -.
DR EnsemblProtists; EAS66934; EAS66934; DDB_G0269474.
DR GeneID; 8616941; -.
DR KEGG; ddi:DDB_G0269474; -.
DR dictyBase; DDB_G0269474; fcsB.
DR eggNOG; KOG1256; Eukaryota.
DR HOGENOM; CLU_000022_45_4_1; -.
DR InParanoid; Q1ZXQ4; -.
DR OMA; CEDPKAK; -.
DR PhylomeDB; Q1ZXQ4; -.
DR Reactome; R-DDI-2046105; Linoleic acid (LA) metabolism.
DR Reactome; R-DDI-2046106; alpha-linolenic acid (ALA) metabolism.
DR Reactome; R-DDI-75876; Synthesis of very long-chain fatty acyl-CoAs.
DR PRO; PR:Q1ZXQ4; -.
DR Proteomes; UP000002195; Chromosome 1.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:dictyBase.
DR GO; GO:0016021; C:integral component of membrane; IDA:dictyBase.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005777; C:peroxisome; IDA:dictyBase.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004467; F:long-chain fatty acid-CoA ligase activity; IMP:dictyBase.
DR GO; GO:0006631; P:fatty acid metabolic process; ISS:dictyBase.
DR GO; GO:0001676; P:long-chain fatty acid metabolic process; IBA:GO_Central.
DR GO; GO:0050766; P:positive regulation of phagocytosis; IMP:dictyBase.
DR CDD; cd05927; LC-FACS_euk; 1.
DR Gene3D; 3.40.50.12780; -; 1.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig.
DR InterPro; IPR042099; ANL_N_sf.
DR InterPro; IPR045311; LC-FACS_euk.
DR Pfam; PF00501; AMP-binding; 1.
DR PROSITE; PS00455; AMP_BINDING; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Endoplasmic reticulum; Fatty acid metabolism; Glycoprotein;
KW Ligase; Lipid metabolism; Nucleotide-binding; Reference proteome; Signal.
FT SIGNAL 1..18
FT /evidence="ECO:0000255"
FT CHAIN 19..673
FT /note="Fatty acyl-CoA synthetase B"
FT /id="PRO_0000384439"
FT CARBOHYD 267
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 673 AA; 75581 MW; 6E1987AAB92B9D8D CRC64;
MINNWLAVGL LVVSGILAFN WKRKHPYGQT VEIGEKPENG GRIRRNSACA DHLISFLEDD
EIYTLYDSLV KSCKKYGERK CFGERKKDSN GNLGKFEWIS YNTYLERCEY IQQGLCELGL
KPKSKVGIFS KNRLEWLIVH SASFIQSYCV VSFYETLGVE SLSYVTEHAE IGLAFCSAET
LQKTLDIAKG VKVLKTIICF DSIDKEHYNI AKELGVTLYT YDEIMKKGKE ANGKHKHTPP
TPDTLSTIMY TSGTTGPPKG VMITHKNLTS VVCAVSDFIK VYDTDVHYSY LPYAHVLERV
VILAAFHFGA AIGIFSGDIS NILVEVKLLS PTLFIGVPRV FERIKTNVFK EISKKPALLR
TLFNGAYNLK YLSIQHGFKL PIIEKVLDLV FFSKIKQALG GKVRVILSGS APLSFDTEVF
LRVVMCCCVL QGYGASEGCG GDACKRLDDE SVGTIGPPFA SNEIKLVDVP ELGYDSNGEV
QTGEVCLRGP SISSGYYKDE EKTREEFKDG WFHTGDIGRW NRDGSLSIVD RKKNIFKLSQ
GEYVAVEKIE TIVVKSEYVE QVCIYGDSQK SCVIAIIHPH PESCSEWAGS KKTDKDIKEI
CKNQDFIKVV LDDIIKNCKK SGLHGFEIPK AIHLTPEAFS DQNNLLTPSF KLKRHEIKKY
FEDEIKKLYS KLD
