FCSK_MOUSE
ID FCSK_MOUSE Reviewed; 1090 AA.
AC Q7TMC8; Q7TSB1; Q8C7W3; Q8CI18;
DT 08-JUN-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=L-fucose kinase {ECO:0000305};
DE Short=Fucokinase {ECO:0000312|MGI:MGI:1916071};
DE EC=2.7.1.52 {ECO:0000269|PubMed:14686921};
GN Name=Fcsk; Synonyms=Fuk {ECO:0000312|MGI:MGI:1916071};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1] {ECO:0000312|EMBL:CAD59135.1}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, CATALYTIC
RP ACTIVITY, AND TISSUE SPECIFICITY.
RC STRAIN=BALB/cJ {ECO:0000312|EMBL:CAD59135.1};
RX PubMed=14686921; DOI=10.1046/j.1432-1033.2003.03904.x;
RA Niittymaki J., Mattila P., Roos C., Huopaniemi L., Sjoblom S., Renkonen R.;
RT "Cloning and expression of murine enzymes involved in the salvage pathway
RT of GDP-L-fucose.";
RL Eur. J. Biochem. 271:78-86(2004).
RN [2] {ECO:0000312|EMBL:AAP20647.1}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J {ECO:0000312|EMBL:AAP20647.1};
RA Jang J.S., Ackerman S.L.;
RT "cDNA sequences of L-fucose kinase.";
RL Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000312|Proteomes:UP000000589}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J {ECO:0000312|Proteomes:UP000000589};
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4] {ECO:0000312|EMBL:EDL11475.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000312|EMBL:AAH37698.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=FVB/N {ECO:0000312|EMBL:AAH37698.1};
RC TISSUE=Salivary gland {ECO:0000312|EMBL:AAH37698.1};
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6] {ECO:0000312|EMBL:BAC33572.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 362-1090 (ISOFORM 1).
RC STRAIN=C57BL/6J {ECO:0000312|EMBL:BAC33572.1};
RC TISSUE=Embryonic stem cell {ECO:0000312|EMBL:BAC33572.1};
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Kidney, Liver, Lung, Pancreas, Spleen,
RC and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Takes part in the salvage pathway for reutilization of fucose
CC from the degradation of oligosaccharides.
CC {ECO:0000305|PubMed:14686921}.
CC -!- FUNCTION: [Isoform 1]: Has strong fucokinase activity.
CC {ECO:0000269|PubMed:14686921}.
CC -!- FUNCTION: [Isoform 2]: Has very weak fucokinase activity.
CC {ECO:0000269|PubMed:14686921}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-fucose = ADP + beta-L-fucose 1-phosphate + H(+);
CC Xref=Rhea:RHEA:13241, ChEBI:CHEBI:2181, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:57268, ChEBI:CHEBI:456216;
CC EC=2.7.1.52; Evidence={ECO:0000269|PubMed:14686921};
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q7TMC8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q7TMC8-2; Sequence=VSP_058365;
CC -!- TISSUE SPECIFICITY: Isoform 1: Expressed strongly in brain, ovary and
CC testis, moderately in kidney and liver, and weakly in lung, spleen and
CC heart. Isoform 2: Expressed very strongly in brain, strongly in ovary,
CC testis and kidney, moderately in lung and liver, and weakly in heart
CC and spleen. {ECO:0000269|PubMed:14686921}.
CC -!- SIMILARITY: Belongs to the GHMP kinase family.
CC {ECO:0000255|RuleBase:RU004209}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH37698.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AJ534942; CAD59135.1; -; mRNA.
DR EMBL; AJ297482; CAC82178.4; -; mRNA.
DR EMBL; AY223865; AAP20647.1; -; mRNA.
DR EMBL; AC132945; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466525; EDL11475.1; -; Genomic_DNA.
DR EMBL; BC037698; AAH37698.1; ALT_INIT; mRNA.
DR EMBL; AK049153; BAC33572.1; -; mRNA.
DR CCDS; CCDS22666.1; -. [Q7TMC8-1]
DR RefSeq; NP_758487.2; NM_172283.3. [Q7TMC8-1]
DR AlphaFoldDB; Q7TMC8; -.
DR STRING; 10090.ENSMUSP00000039271; -.
DR iPTMnet; Q7TMC8; -.
DR PhosphoSitePlus; Q7TMC8; -.
DR EPD; Q7TMC8; -.
DR MaxQB; Q7TMC8; -.
DR PaxDb; Q7TMC8; -.
DR PeptideAtlas; Q7TMC8; -.
DR PRIDE; Q7TMC8; -.
DR ProteomicsDB; 271611; -. [Q7TMC8-1]
DR ProteomicsDB; 271612; -. [Q7TMC8-2]
DR Antibodypedia; 29979; 160 antibodies from 27 providers.
DR DNASU; 234730; -.
DR Ensembl; ENSMUST00000041382; ENSMUSP00000039271; ENSMUSG00000033703. [Q7TMC8-1]
DR GeneID; 234730; -.
DR KEGG; mmu:234730; -.
DR UCSC; uc009nlg.2; mouse. [Q7TMC8-1]
DR CTD; 197258; -.
DR MGI; MGI:1916071; Fcsk.
DR VEuPathDB; HostDB:ENSMUSG00000033703; -.
DR eggNOG; KOG4644; Eukaryota.
DR GeneTree; ENSGT00390000002251; -.
DR HOGENOM; CLU_006983_0_0_1; -.
DR InParanoid; Q7TMC8; -.
DR OMA; QRWREAW; -.
DR OrthoDB; 135001at2759; -.
DR PhylomeDB; Q7TMC8; -.
DR TreeFam; TF314554; -.
DR BRENDA; 2.7.1.52; 3474.
DR Reactome; R-MMU-6787639; GDP-fucose biosynthesis.
DR BioGRID-ORCS; 234730; 1 hit in 72 CRISPR screens.
DR PRO; PR:Q7TMC8; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q7TMC8; protein.
DR Bgee; ENSMUSG00000033703; Expressed in paneth cell and 222 other tissues.
DR ExpressionAtlas; Q7TMC8; baseline and differential.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0050201; F:fucokinase activity; IDA:UniProtKB.
DR GO; GO:0046835; P:carbohydrate phosphorylation; IDA:UniProtKB.
DR GO; GO:0042352; P:GDP-L-fucose salvage; ISS:UniProtKB.
DR GO; GO:1903350; P:response to dopamine; IEA:Ensembl.
DR InterPro; IPR012887; Fucokinase.
DR InterPro; IPR013750; GHMP_kinase_C_dom.
DR InterPro; IPR036554; GHMP_kinase_C_sf.
DR InterPro; IPR006204; GHMP_kinase_N_dom.
DR InterPro; IPR020568; Ribosomal_S5_D2-typ_fold.
DR Pfam; PF07959; Fucokinase; 1.
DR Pfam; PF08544; GHMP_kinases_C; 1.
DR Pfam; PF00288; GHMP_kinases_N; 1.
DR SUPFAM; SSF54211; SSF54211; 1.
DR SUPFAM; SSF55060; SSF55060; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; ATP-binding; Kinase; Nucleotide-binding;
KW Reference proteome; Transferase.
FT CHAIN 1..1090
FT /note="L-fucose kinase"
FT /id="PRO_0000436383"
FT BINDING 834..845
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT VAR_SEQ 923..993
FT /note="Missing (in isoform 2)"
FT /id="VSP_058365"
SQ SEQUENCE 1090 AA; 119267 MW; 46A1FA0750424B9E CRC64;
MEQSEGVNWT VIILTCQYKD SVQVFQRELE VRQRREQIPA GTMLLAVEDP QTRVGSGGAT
LNALLVAAEH LSARAGFTVV TSDVLHSAWI LILHMGRDFP FDDCGRAFTC LPVENPQAPV
EALVCNLDCL LDIMTHRLGP GSPPGVWVCS TDMLLSVPPN PGISWDGFRG ARVIAFPGSL
AYALNHGVYL TDSQGLVLDI YYQGTKAEIQ RCVGPDGLVP LVSGVVFFSV ETAEHLLATH
VSPPLDACTY MGLDSGAQPV QLSLFFDILL CMARNMSREN FLAGRPPELG QGDMDVASYL
KGARAQLWRE LRDQPLTMVY VPDGGYSYMT TDATEFLHRL TMPGVAVAQI VHSQVEEPQL
LEATCSVVSC LLEGPVHLGP RSVLQHCHLR GPIRIGAGCF VSGLDTAHSE ALHGLELHDV
ILQGHHVRLH GSLSRVFTLA GRLDSWERQG AGMYLNMSWN EFFKKTGIRD WDLWDPDTPP
SDRCLLTARL FPVLHPTRAL GPQDVLWMLH PRKHRGEALR AWRASWRLSW EQLQPCVDRA
ATLDFRRDLF FCQALQKARH VLEARQDLCL RPLIRAAVGE GCSGPLLATL DKVAAGAEDP
GVAARALACV ADVLGCMAEG RGGLRSGPAA NPEWIQPFSY LECGDLMRGV EALAQEREKW
LTRPALLVRA ARHYEGAEQI LIRQAVMTAR HFVSTQPVEL PAPGQWVVTE CPARVDFSGG
WSDTPPIAYE LGGAVLGLAV RVDGRRPIGA KARRIPEPEL WLAVGPRQDE MTMRIVCRSL
DDLRDYCQPH APGALLKAAF ICAGIVHLHS ELPLLEQLLH SFNGGFELHT WSELPHGSGL
GTSSILAGAA LAALQRAAGR AVGTEALIHA VLHLEQVLTT GGGWQDQVSG LMPGIKVGRS
RAQLPLKVEV EEITVPEGFV QKINDHLLLV YTGKTRLARN LLQDVLRNWY ARLPVVVQNA
RRLVRQTEKC AEAFRQGNLP LLGQYLTSYW EQKKLMAPGC EPLAVQRMMD VLAPYAYGQS
LAGAGGGGFL YLLTKEPRQK ETLEAVLAKA EGLGNYSVHL VEVDPQGLSL QLLGHDTRLC
GAGPSEVGTT
