FD4L2_ARATH
ID FD4L2_ARATH Reviewed; 279 AA.
AC O81006; Q6DR28;
DT 09-JUL-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Fatty acid desaturase 4-like 2, chloroplastic;
DE Short=FAD4-L2;
DE EC=1.14.19.-;
DE Flags: Precursor;
GN Name=FAD4L2; OrderedLocusNames=At2g22890; ORFNames=T20K9.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=16244158; DOI=10.1104/pp.105.063479;
RA Xiao Y.-L., Smith S.R., Ishmael N., Redman J.C., Kumar N., Monaghan E.L.,
RA Ayele M., Haas B.J., Wu H.C., Town C.D.;
RT "Analysis of the cDNAs of hypothetical genes on Arabidopsis chromosome 2
RT reveals numerous transcript variants.";
RL Plant Physiol. 139:1323-1337(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RA Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W.,
RA Redman J.C., Wu H.C., Utterback T., Town C.D.;
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=19682287; DOI=10.1111/j.1365-313x.2009.04001.x;
RA Gao J., Ajjawi I., Manoli A., Sawin A., Xu C., Froehlich J.E., Last R.L.,
RA Benning C.;
RT "FATTY ACID DESATURASE4 of Arabidopsis encodes a protein distinct from
RT characterized fatty acid desaturases.";
RL Plant J. 60:832-839(2009).
CC -!- FUNCTION: Fatty acid desaturase involved in the production of
CC chloroplast-specific phosphatidylglycerol molecular species. Catalyzes
CC the formation of a trans double bond introduced close to the carboxyl
CC group of palmitic acid, which is specifically esterified to the sn-2
CC glyceryl carbon of phosphatidylglycerol (By similarity). {ECO:0000250}.
CC -!- PATHWAY: Lipid metabolism; fatty acid metabolism.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane {ECO:0000250};
CC Multi-pass membrane protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=O81006-1; Sequence=Displayed;
CC Name=2;
CC IsoId=O81006-2; Sequence=VSP_055334;
CC -!- SIMILARITY: Belongs to the fatty acid desaturase CarF family.
CC {ECO:0000305}.
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DR EMBL; AC004786; AAC32436.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07369.1; -; Genomic_DNA.
DR EMBL; AY600553; AAT68352.1; -; mRNA.
DR EMBL; AY649287; AAT69204.1; -; Genomic_DNA.
DR PIR; B84618; B84618.
DR RefSeq; NP_179874.1; NM_127854.3. [O81006-1]
DR AlphaFoldDB; O81006; -.
DR STRING; 3702.AT2G22890.1; -.
DR PaxDb; O81006; -.
DR PRIDE; O81006; -.
DR EnsemblPlants; AT2G22890.1; AT2G22890.1; AT2G22890. [O81006-1]
DR GeneID; 816820; -.
DR Gramene; AT2G22890.1; AT2G22890.1; AT2G22890. [O81006-1]
DR KEGG; ath:AT2G22890; -.
DR Araport; AT2G22890; -.
DR TAIR; locus:2059149; AT2G22890.
DR eggNOG; KOG3011; Eukaryota.
DR HOGENOM; CLU_065233_0_0_1; -.
DR InParanoid; O81006; -.
DR OMA; VGTHIED; -.
DR OrthoDB; 1421561at2759; -.
DR PhylomeDB; O81006; -.
DR UniPathway; UPA00199; -.
DR PRO; PR:O81006; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O81006; baseline and differential.
DR Genevisible; O81006; AT.
DR GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0006631; P:fatty acid metabolic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR019547; Lipid_desat.
DR Pfam; PF10520; Lipid_desat; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Chloroplast; Fatty acid metabolism; Lipid metabolism;
KW Membrane; Oxidoreductase; Plastid; Reference proteome; Transit peptide;
KW Transmembrane; Transmembrane helix.
FT TRANSIT 1..30
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 31..279
FT /note="Fatty acid desaturase 4-like 2, chloroplastic"
FT /id="PRO_0000429929"
FT TRANSMEM 68..90
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 98..118
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 178..198
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VAR_SEQ 231..279
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16244158"
FT /id="VSP_055334"
SQ SEQUENCE 279 AA; 31505 MW; D364C5C72CAA68F7 CRC64;
MATSLQTKYT LNPITNNIPR SHRPSFLRVT STTNSQPNHE MKLVVEQRLV NPPLSNDPTL
QSTWTHRLWV AAGCTTVFVS FSKSIIGAFG SHLWLEPSLA GFAGYILADL GSGVYHWATD
NYGDESTPLV GIHIEDSQDH HKCPWTITKR QFANNLHFMA RGTTLIVLPL DLAFDDHVVH
GFVSMFAFCV LFCQLFHAWA HGTKSKLPPL VVGLQDIGLL VSRIHHMNHH RAPYNNNYCV
VSGVWNKVLD ESNVFKAMEM VLYIQLGVRP RSWTEPNYE
