ID   FD6E1_SOYBN             Reviewed;         387 AA.
AC   P48630;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   03-AUG-2022, entry version 113.
DE   RecName: Full=Omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 1;
DE            EC=1.14.19.-;
GN   Name=FAD2-1;
OS   Glycine max (Soybean) (Glycine hispida).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC   Glycine subgen. Soja.
OX   NCBI_TaxID=3847;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Seed;
RX   PubMed=8587990; DOI=10.1104/pp.110.1.311;
RA   Heppard E.P., Kinney A.J., Stecca K.L., Miao G.H.;
RT   "Developmental and growth temperature regulation of two different
RT   microsomal omega-6 desaturase genes in soybeans.";
RL   Plant Physiol. 110:311-319(1996).
CC   -!- FUNCTION: ER (microsomal) omega-6 fatty acid desaturase introduces the
CC       second double bond in the biosynthesis of 18:3 fatty acids, important
CC       constituents of plant membranes. It is thought to use cytochrome b5 as
CC       an electron donor and to act on fatty acids esterified to
CC       phosphatidylcholine and, possibly, other phospholipids.
CC   -!- PATHWAY: Lipid metabolism; polyunsaturated fatty acid biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass
CC       membrane protein.
CC   -!- TISSUE SPECIFICITY: Strongly expressed in developing seeds.
CC   -!- DOMAIN: The histidine box domains may contain the active site and/or be
CC       involved in metal ion binding.
CC   -!- SIMILARITY: Belongs to the fatty acid desaturase type 1 family.
CC       {ECO:0000305}.
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DR   EMBL; L43920; AAB00859.1; -; mRNA.
DR   PIR; T07687; T07687.
DR   RefSeq; NP_001238342.1; NM_001251413.1.
DR   RefSeq; XP_006588506.1; XM_006588443.1.
DR   AlphaFoldDB; P48630; -.
DR   STRING; 3847.GLYMA10G42470.1; -.
DR   PRIDE; P48630; -.
DR   EnsemblPlants; KRH36003; KRH36003; GLYMA_10G278000.
DR   EnsemblPlants; KRH36004; KRH36004; GLYMA_10G278000.
DR   GeneID; 547814; -.
DR   Gramene; KRH36003; KRH36003; GLYMA_10G278000.
DR   Gramene; KRH36004; KRH36004; GLYMA_10G278000.
DR   KEGG; gmx:547814; -.
DR   eggNOG; ENOG502QQNB; Eukaryota.
DR   HOGENOM; CLU_033094_0_1_1; -.
DR   InParanoid; P48630; -.
DR   OMA; ECLYIVR; -.
DR   OrthoDB; 577374at2759; -.
DR   UniPathway; UPA00658; -.
DR   Proteomes; UP000008827; Chromosome 10.
DR   Genevisible; P48630; GM.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006636; P:unsaturated fatty acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR005804; FA_desaturase_dom.
DR   Pfam; PF00487; FA_desaturase; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Fatty acid biosynthesis; Fatty acid metabolism;
KW   Lipid biosynthesis; Lipid metabolism; Membrane; Oxidoreductase;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..387
FT                   /note="Omega-6 fatty acid desaturase, endoplasmic reticulum
FT                   isozyme 1"
FT                   /id="PRO_0000185420"
FT   TRANSMEM        54..74
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        87..107
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        121..141
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        183..203
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        227..247
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        251..271
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   MOTIF           109..113
FT                   /note="Histidine box-1"
FT   MOTIF           145..149
FT                   /note="Histidine box-2"
FT   MOTIF           319..323
FT                   /note="Histidine box-3"
SQ   SEQUENCE   387 AA;  44663 MW;  49068805C21A1C31 CRC64;
     MGLAKETTMG GRGRVAKVEV QGKKPLSRVP NTKPPFTVGQ LKKAIPPHCF QRSLLTSFSY
     VVYDLSFAFI FYIATTYFHL LPQPFSLIAW PIYWVLQGCL LTGVWVIAHE CGHHAFSKYQ
     WVDDVVGLTL HSTLLVPYFS WKISHRRHHS NTGSLDRDEV FVPKPKSKVA WFSKYLNNPL
     GRAVSLLVTL TIGWPMYLAF NVSGRPYDSF ASHYHPYAPI YSNRERLLIY VSDVALFSVT
     YSLYRVATLK GLVWLLCVYG VPLLIVNGFL VTITYLQHTH FALPHYDSSE WDWLKGALAT
     MDRDYGILNK VFHHITDTHV AHHLFSTMPH YHAMEATNAI KPILGEYYQF DDTPFYKALW
     REARECLYVE PDEGTSEKGV YWYRNKY