ID   FD6E2_SOYBN             Reviewed;         383 AA.
AC   P48631;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=Omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2;
DE            EC=1.14.19.-;
GN   Name=FAD2-2;
OS   Glycine max (Soybean) (Glycine hispida).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC   Glycine subgen. Soja.
OX   NCBI_TaxID=3847;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Epicotyl;
RX   PubMed=8587990; DOI=10.1104/pp.110.1.311;
RA   Heppard E.P., Kinney A.J., Stecca K.L., Miao G.H.;
RT   "Developmental and growth temperature regulation of two different
RT   microsomal omega-6 desaturase genes in soybeans.";
RL   Plant Physiol. 110:311-319(1996).
CC   -!- FUNCTION: ER (microsomal) omega-6 fatty acid desaturase introduces the
CC       second double bond in the biosynthesis of 18:3 fatty acids, important
CC       constituents of plant membranes. It is thought to use cytochrome b5 as
CC       an electron donor and to act on fatty acids esterified to
CC       phosphatidylcholine and, possibly, other phospholipids.
CC   -!- PATHWAY: Lipid metabolism; polyunsaturated fatty acid biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass
CC       membrane protein.
CC   -!- DOMAIN: The histidine box domains may contain the active site and/or be
CC       involved in metal ion binding.
CC   -!- SIMILARITY: Belongs to the fatty acid desaturase type 1 family.
CC       {ECO:0000305}.
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DR   EMBL; L43921; AAB00860.1; -; mRNA.
DR   PIR; T07688; T07688.
DR   RefSeq; NP_001238358.1; NM_001251429.1.
DR   AlphaFoldDB; P48631; -.
DR   STRING; 3847.GLYMA03G30070.3; -.
DR   PRIDE; P48631; -.
DR   GeneID; 547815; -.
DR   KEGG; gmx:547815; -.
DR   eggNOG; ENOG502QQNB; Eukaryota.
DR   HOGENOM; CLU_033094_0_1_1; -.
DR   InParanoid; P48631; -.
DR   OrthoDB; 577374at2759; -.
DR   BRENDA; 1.14.19.6; 2483.
DR   UniPathway; UPA00658; -.
DR   Proteomes; UP000008827; Unplaced.
DR   Genevisible; P48631; GM.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016717; F:oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water; IEA:InterPro.
DR   GO; GO:0006636; P:unsaturated fatty acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR005804; FA_desaturase_dom.
DR   InterPro; IPR021863; FAS_N.
DR   Pfam; PF11960; DUF3474; 1.
DR   Pfam; PF00487; FA_desaturase; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Fatty acid biosynthesis; Fatty acid metabolism;
KW   Lipid biosynthesis; Lipid metabolism; Membrane; Oxidoreductase;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..383
FT                   /note="Omega-6 fatty acid desaturase, endoplasmic reticulum
FT                   isozyme 2"
FT                   /id="PRO_0000185421"
FT   TRANSMEM        61..81
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        85..105
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        117..137
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        179..199
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        225..245
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        249..269
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   MOTIF           105..109
FT                   /note="Histidine box-1"
FT   MOTIF           141..145
FT                   /note="Histidine box-2"
FT   MOTIF           315..319
FT                   /note="Histidine box-3"
SQ   SEQUENCE   383 AA;  43968 MW;  F23EF7159B2F9967 CRC64;
     MGAGGRTDVP PANRKSEVDP LKRVPFEKPQ FSLSQIKKAI PPHCFQRSVL RSFSYVVYDL
     TIAFCLYYVA THYFHLLPGP LSFRGMAIYW AVQGCILTGV WVIAHECGHH AFSDYQLLDD
     IVGLILHSAL LVPYFSWKYS HRRHHSNTGS LERDEVFVPK QKSCIKWYSK YLNNPPGRVL
     TLAVTLTLGW PLYLALNVSG RPYDRFACHY DPYGPIYSDR ERLQIYISDA GVLAVVYGLF
     RLAMAKGLAW VVCVYGVPLL VVNGFLVLIT FLQHTHPALP HYTSSEWDWL RGALATVDRD
     YGILNKVFHN ITDTHVAHHL FSTMPHYHAM EATKAIKPIL GEYYRFDETP FVKAMWREAR
     ECIYVEPDQS TESKGVFWYN NKL