ID   FDB21_FUSPC             Reviewed;         902 AA.
AC   K3VZ28;
DT   23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT   28-NOV-2012, sequence version 1.
DT   03-AUG-2022, entry version 54.
DE   RecName: Full=Transcription factor FPSE_08121 {ECO:0000303|PubMed:25727347};
DE   AltName: Full=Fusarium detoxification of benzoxazolinone cluster protein FPSE_08121 {ECO:0000303|PubMed:25727347};
DE            Short=FDB cluster protein FPSE_08121 {ECO:0000303|PubMed:25727347};
GN   ORFNames=FPSE_08121;
OS   Fusarium pseudograminearum (strain CS3096) (Wheat and barley crown-rot
OS   fungus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium.
OX   NCBI_TaxID=1028729;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CS3096;
RX   PubMed=23028337; DOI=10.1371/journal.ppat.1002952;
RA   Gardiner D.M., McDonald M.C., Covarelli L., Solomon P.S., Rusu A.G.,
RA   Marshall M., Kazan K., Chakraborty S., McDonald B.A., Manners J.M.;
RT   "Comparative pathogenomics reveals horizontally acquired novel virulence
RT   genes in fungi infecting cereal hosts.";
RL   PLoS Pathog. 8:E1002952-E1002952(2012).
RN   [2]
RP   FUNCTION, AND INDUCTION.
RX   PubMed=25727347; DOI=10.1111/mpp.12250;
RA   Kettle A.J., Batley J., Benfield A.H., Manners J.M., Kazan K.,
RA   Gardiner D.M.;
RT   "Degradation of the benzoxazolinone class of phytoalexins is important for
RT   virulence of Fusarium pseudograminearum towards wheat.";
RL   Mol. Plant Pathol. 16:946-962(2015).
RN   [3]
RP   FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=26828593; DOI=10.1016/j.fgb.2016.01.015;
RA   Kettle A.J., Carere J., Batley J., Manners J.M., Kazan K., Gardiner D.M.;
RT   "The Fdb3 transcription factor of the Fusarium Detoxification of
RT   Benzoxazolinone gene cluster is required for MBOA but not BOA degradation
RT   in Fusarium pseudograminearum.";
RL   Fungal Genet. Biol. 88:44-53(2016).
CC   -!- FUNCTION: Transcription factor; part of the Fusarium detoxification of
CC       benzoxazolinone cluster involved in the degradation of benzoxazolinones
CC       produced by the host plant (PubMed:25727347, PubMed:26828593). Maize,
CC       wheat, and rye produce the 2 benzoxazinone phytoanticipins 2,4-dihy-
CC       droxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA) and 2,4-dihydroxy-1,4-
CC       benzoxazin-3-one (DIBOA) that, due to their inherent instability once
CC       released, spontaneously degrade to the more stable corresponding
CC       benzoxazolinones, 6-methoxy-2-benzoxazolinone (MBOA) and 2-
CC       benzoxazolinone (BOA), respectively (By similarity). FPSE_08121
CC       positively regulates the expression of the FBD cluster gene FPSE_08120
CC       in response to 2-aminophenol (2-AP) treatment and contributes
CC       quantitatively to benzoxazolinone tolerance (PubMed:26828593).
CC       {ECO:0000250|UniProtKB:W7MLD5, ECO:0000269|PubMed:25727347,
CC       ECO:0000269|PubMed:26828593}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00227}.
CC   -!- INDUCTION: Expression is induced in response to 2-benzoxasolinone (BOA)
CC       exposure (PubMed:25727347). Expression is also induced in response to
CC       6-methoxy-2-benzoxazolinone (MBOA) and 2-aminophenol (2-AP) treatment
CC       (PubMed:26828593). {ECO:0000269|PubMed:25727347,
CC       ECO:0000269|PubMed:26828593}.
CC   -!- DISRUPTION PHENOTYPE: Reduces significantly the induction of the FBD
CC       cluster gene FPSE_08120 in response to 2-aminophenol (2-AP) treatment.
CC       {ECO:0000269|PubMed:26828593}.
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DR   EMBL; AFNW01000283; EKJ71675.1; -; Genomic_DNA.
DR   RefSeq; XP_009259514.1; XM_009261239.1.
DR   EnsemblFungi; EKJ71675; EKJ71675; FPSE_08121.
DR   GeneID; 20366739; -.
DR   KEGG; fpu:FPSE_08121; -.
DR   eggNOG; ENOG502RHAD; Eukaryota.
DR   HOGENOM; CLU_006329_1_4_1; -.
DR   Proteomes; UP000007978; Chromosome 2.
DR   Proteomes; UP000007978; Unassembled WGS sequence.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd00067; GAL4; 1.
DR   Gene3D; 4.10.240.10; -; 1.
DR   InterPro; IPR007219; Transcription_factor_dom_fun.
DR   InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR   InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR   Pfam; PF04082; Fungal_trans; 1.
DR   Pfam; PF00172; Zn_clus; 1.
DR   SMART; SM00906; Fungal_trans; 1.
DR   SMART; SM00066; GAL4; 1.
DR   SUPFAM; SSF57701; SSF57701; 1.
DR   PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR   PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE   2: Evidence at transcript level;
KW   Metal-binding; Nucleus; Zinc.
FT   CHAIN           1..902
FT                   /note="Transcription factor FPSE_08121"
FT                   /id="PRO_0000454604"
FT   DNA_BIND        52..81
FT                   /note="Zn(2)-C6 fungal-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT   REGION          1..47
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          92..189
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          208..234
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          258..277
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          820..839
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..17
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        97..122
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        137..159
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        824..839
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   902 AA;  99212 MW;  208F19FD79B42451 CRC64;
     MVSPDNRPGT QGPSASAHAH DSRVPRKRPG SWDNNPSTAG NRAVKKRAVR ACVSCRDRKV
     RCDVVNGGPP CTNCRLDDVD CVLKASNRGK HNPARYQARS RLSSNATAPR APSPSDNINT
     DADTAAPGPA PGSASATVFR SDHSHGSVAT GQPQERRGSQ SVAHGDEETI CDDDENENNS
     WHNQQEAEQT RIIHDSQVQP NDIVHETHCE QQPQPQPSQG AATRSTPSAQ PANPQTSDYL
     VALAFQGFYA LGWTEQDATE APPSSSRMDN VSASANTPHA DQNHLPLYIS PHPSHLDKHD
     LEFLARKDCL TIPDNQLRDE LIRIYVFVVY PFMPAIDLVD FLEPITGSSE VGTVSLLLFQ
     AIMFASVTFI DMQLLQRYGF KNKRAARQVF FNRVKLLYSL GYEADRLTLV QSLLLMTYWY
     DSDSDEKHTW YWMGLALTTA HIEGLQRDLE EPQQMTKNGR LRRRIWWSCV IRDRLLGLGL
     RRPSRIQEDE FSVERLRLDD FDISLPPPPA VARLLAAPSY TGKDPVNRQR MANLCIDLSQ
     LCFTIGRILH TQYTIASTPG AGSNYLRRAI VRPRSLKEQA HSFAKCDADL QEWFRSLAPE
     SRYVPGARDG GAAAAQVENS TIRLHKILLY MKYLTAIGAL HRPQVFYSGS DSIDPARKAD
     SRRKLTEAAV AITKLAFDLQ SNGQMCYAPT SSVPAFLSAA LIHLLNIRSP DEETRNISIG
     RFCQCLDALH QLQSMYTAAD EAVHIINNMT ENAGFILPLL GIGNPMSKAN GIAARNSRLF
     SAIGPSRVTT AYPSPAPATG NLDTSQEEMI SAPIPGAGPT GVALTGQRSR QPSAGPNMTP
     SATPNLMSIW SAAGNIHSER DLPLPADLMA GIQLDLDAWY NIGDIIDPAL MNFETGLDFL
     NS