FDB2_GIBMO
ID FDB2_GIBMO Reviewed; 345 AA.
AC B7SP66;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 10-FEB-2009, sequence version 1.
DT 25-MAY-2022, entry version 34.
DE RecName: Full=N-malonyltransferase FDB2 {ECO:0000303|PubMed:19302487};
DE EC=2.3.1.- {ECO:0000269|PubMed:19302487};
DE AltName: Full=Fusarium detoxification of benzoxazolinone cluster 2 protein NAT1 {ECO:0000303|PubMed:19302487};
DE Short=FDB2 cluster protein NAT1 {ECO:0000303|PubMed:19302487};
GN Name=FDB2 {ECO:0000303|PubMed:19302487};
GN Synonyms=NAT1 {ECO:0000303|PubMed:19302487};
GN ORFNames=FVEG_12636, FVER53263_12636, FVER53590_12636;
OS Gibberella moniliformis (Maize ear and stalk rot fungus) (Fusarium
OS verticillioides).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium;
OC Fusarium fujikuroi species complex.
OX NCBI_TaxID=117187;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DISRUPTION PHENOTYPE,
RP INDUCTION, AND PATHWAY.
RX PubMed=19302487; DOI=10.1111/j.1365-2672.2009.04246.x;
RA Glenn A.E., Bacon C.W.;
RT "FDB2 encodes a member of the arylamine N-acetyltransferase family and is
RT necessary for biotransformation of benzoxazolinones by Fusarium
RT verticillioides.";
RL J. Appl. Microbiol. 107:657-671(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=20621844; DOI=10.1016/j.febslet.2010.05.063;
RA Glenn A.E., Karagianni E.P., Ulndreaj A., Boukouvala S.;
RT "Comparative genomic and phylogenetic investigation of the xenobiotic
RT metabolizing arylamine N-acetyltransferase enzyme family.";
RL FEBS Lett. 584:3158-3164(2010).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=M3125 / FGSC 7600;
RA Karagianni E.P., Ormiston B., Ouldreou A., Glenn A.E., Boukouvala S.;
RT "Genes for xenobiotic metabolising enzymes in fungi: The family of
RT arylamine N-acetyltransferases (NATs).";
RL Submitted (MAR-2010) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BRIP53263;
RA Gardiner D.;
RT "Genome of an Australian isolate of Fusarium verticillioides BRIP53263.";
RL Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION.
RX PubMed=11876429; DOI=10.1094/mpmi.2002.15.2.91;
RA Glenn A.E., Gold S.E., Bacon C.W.;
RT "Fdb1 and Fdb2, Fusarium verticillioides loci necessary for detoxification
RT of preformed antimicrobials from corn.";
RL Mol. Plant Microbe Interact. 15:91-101(2002).
RN [6]
RP FUNCTION.
RX PubMed=12788712; DOI=10.1128/aem.69.6.3165-3169.2003;
RA Glenn A.E., Meredith F.I., Morrison W.H. III, Bacon C.W.;
RT "Identification of intermediate and branch metabolites resulting from
RT biotransformation of 2-benzoxazolinone by Fusarium verticillioides.";
RL Appl. Environ. Microbiol. 69:3165-3169(2003).
RN [7]
RP FUNCTION.
RX PubMed=26808652; DOI=10.1371/journal.pone.0147486;
RA Glenn A.E., Davis C.B., Gao M., Gold S.E., Mitchell T.R., Proctor R.H.,
RA Stewart J.E., Snook M.E.;
RT "Two horizontally transferred xenobiotic resistance gene clusters
RT associated with detoxification of benzoxazolinones by Fusarium species.";
RL PLoS ONE 11:e0147486-e0147486(2016).
CC -!- FUNCTION: N-malonyltransferase; part of the Fusarium detoxification of
CC benzoxazolinone cluster 2 (FDB2) involved in the degradation of
CC benzoxazolinones produced by the host plant (PubMed:19302487,
CC PubMed:26808652). Maize, wheat, and rye produce the 2 benzoxazinone
CC phytoanticipins 2,4-dihy-droxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA)
CC and 2,4-dihydroxy-1,4-benzoxazin-3-one (DIBOA) that, due to their
CC inherent instability once released, spontaneously degrade to the more
CC stable corresponding benzoxazolinones, 6-methoxy-2-benzoxazolinone
CC (MBOA) and 2-benzoxazolinone (BOA), respectively (PubMed:11876429). The
CC first step in the detoxification of benzoxazolinones involves the
CC hydrolysis of the cyclic ester bond of benzoxazolinones by the FDB1
CC cluster gamma-lactamase MBL1 to aminophenols (PubMed:26808652,
CC PubMed:12788712). MBL1 is able to convert BOA into 2-aminophenol (2-
CC AP), as well as MBOA into 5-methoxy-2-aminophenol (2-AMP)
CC (PubMed:26808652, PubMed:12788712). The FDB2 cluster N-
CC malonyltransferase FDB2/NAT1 then metabolizes aminophenols via N-
CC malonylation to non-toxic malonamic acids (PubMed:19302487,
CC PubMed:12788712). FDB2/NAT1 converts 2-AP into N-(2-hydroxyphenyl)
CC malonamic acid (HPMA) and 2-AMP into N-(2-hydroxy-4-methoxyphenyl)
CC malonamic acid (HMPMA) (PubMed:19302487, PubMed:12788712). The
CC duplicated dienlactone hydrolases DLH1 and DLH2 may provide redundant
CC function for hydrolyzing the lactone moiety in the BOA molecule
CC (Probable). The roles of the amidases an other enzymes encoded by the 2
CC FDB clusters have not been identified so far (Probable).
CC {ECO:0000269|PubMed:11876429, ECO:0000269|PubMed:12788712,
CC ECO:0000269|PubMed:19302487, ECO:0000269|PubMed:26808652,
CC ECO:0000305|PubMed:26808652}.
CC -!- PATHWAY: Xenobiotic degradation. {ECO:0000269|PubMed:19302487}.
CC -!- INDUCTION: Expression is induced in response to 2-benzoxasolinone (BOA)
CC exposure. {ECO:0000269|PubMed:19302487}.
CC -!- DISRUPTION PHENOTYPE: Eliminates the ability to metabolize BOA.
CC {ECO:0000269|PubMed:19302487}.
CC -!- MISCELLANEOUS: Fusarium verticillioides possesses 2 unlinked loci, FDB1
CC and FDB2, necessary for detoxification of antimicrobial compounds
CC produced by maize, including 2-benzoxazolinone (BOA) (Probable). The
CC FDB2 cluster arose as a duplication of the FDB1 cluster with
CC rearrangement and expansion by incorporating additional genes
CC (Probable). {ECO:0000305|PubMed:26808652}.
CC -!- SIMILARITY: Belongs to the arylamine N-acetyltransferase family.
CC {ECO:0000305}.
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DR EMBL; EU552489; ACD88491.1; -; Genomic_DNA.
DR EMBL; FN687904; CBK52381.1; -; mRNA.
DR EMBL; BN001430; CBL43336.1; -; Genomic_DNA.
DR EMBL; QJUS01000105; RBQ97400.1; -; Genomic_DNA.
DR EMBL; QKXB01000334; RBR06778.1; -; Genomic_DNA.
DR OMA; MSPYPEV; -.
DR Proteomes; UP000252067; Chromosome 3.
DR Proteomes; UP000252067; Unassembled WGS sequence.
DR Proteomes; UP000253456; Chromosome 3.
DR Proteomes; UP000253456; Unassembled WGS sequence.
DR GO; GO:0004060; F:arylamine N-acetyltransferase activity; IEA:UniProtKB-EC.
DR InterPro; IPR001447; Arylamine_N-AcTrfase.
DR InterPro; IPR038765; Papain-like_cys_pep_sf.
DR PANTHER; PTHR11786; PTHR11786; 1.
DR Pfam; PF00797; Acetyltransf_2; 1.
DR PRINTS; PR01543; ANATRNSFRASE.
DR SUPFAM; SSF54001; SSF54001; 1.
PE 2: Evidence at transcript level;
KW Acyltransferase; Transferase.
FT CHAIN 1..345
FT /note="N-malonyltransferase FDB2"
FT /id="PRO_0000454596"
FT ACT_SITE 110
FT /note="Acyl-thioester intermediate"
FT /evidence="ECO:0000250|UniProtKB:P9WJI5"
FT ACT_SITE 158
FT /note="Proton acceptor"
FT /evidence="ECO:0000250|UniProtKB:P9WJI5"
FT ACT_SITE 173
FT /evidence="ECO:0000250|UniProtKB:P9WJI5"
SQ SEQUENCE 345 AA; 39734 MW; E667B20BF7D34F16 CRC64;
MARLEDPTAL TQLPDESARV RYTSSELQDY FETLKFPQRF LDLGNSVLKD PSLARTKENG
LPLLQAITRY HTCNVPFENL VLHYDPHKIV TLDPAELYTK IVTRRRGGRC MENNIFLGTA
LRSLGYEVRN CGGRVSRAMS PYPEVRKNQS ATYDGWNHML LLVFLGDEWY GVDVGMGSMG
PNLPFPLQDG FESLSIAPRE IRIQKRSISE THATGPSHAT KMWCYDVCYN PAESKKTWTP
VYCFTETEFL PQDYEVMSWF TSTNPRSFFT RYITCTKMIM DEDKEVIIGN LTLFKDTVRE
TIGSDRKVVK KFETEEERIK GLVEIFDVNL TEEEKNSLPQ EKRLA
