FDB3_FUSPC
ID FDB3_FUSPC Reviewed; 757 AA.
AC K3UIH8;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 28-NOV-2012, sequence version 1.
DT 03-AUG-2022, entry version 57.
DE RecName: Full=Transcription factor FBD3 {ECO:0000303|PubMed:26828593};
DE AltName: Full=Fusarium detoxification of benzoxazolinone cluster protein 3 {ECO:0000303|PubMed:25727347};
DE Short=FDB cluster protein 3 {ECO:0000303|PubMed:25727347};
GN Name=FDB3 {ECO:0000303|PubMed:26828593}; ORFNames=FPSE_08122;
OS Fusarium pseudograminearum (strain CS3096) (Wheat and barley crown-rot
OS fungus).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium.
OX NCBI_TaxID=1028729;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CS3096;
RX PubMed=23028337; DOI=10.1371/journal.ppat.1002952;
RA Gardiner D.M., McDonald M.C., Covarelli L., Solomon P.S., Rusu A.G.,
RA Marshall M., Kazan K., Chakraborty S., McDonald B.A., Manners J.M.;
RT "Comparative pathogenomics reveals horizontally acquired novel virulence
RT genes in fungi infecting cereal hosts.";
RL PLoS Pathog. 8:E1002952-E1002952(2012).
RN [2]
RP FUNCTION, AND INDUCTION.
RX PubMed=25727347; DOI=10.1111/mpp.12250;
RA Kettle A.J., Batley J., Benfield A.H., Manners J.M., Kazan K.,
RA Gardiner D.M.;
RT "Degradation of the benzoxazolinone class of phytoalexins is important for
RT virulence of Fusarium pseudograminearum towards wheat.";
RL Mol. Plant Pathol. 16:946-962(2015).
RN [3]
RP FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=26828593; DOI=10.1016/j.fgb.2016.01.015;
RA Kettle A.J., Carere J., Batley J., Manners J.M., Kazan K., Gardiner D.M.;
RT "The Fdb3 transcription factor of the Fusarium Detoxification of
RT Benzoxazolinone gene cluster is required for MBOA but not BOA degradation
RT in Fusarium pseudograminearum.";
RL Fungal Genet. Biol. 88:44-53(2016).
CC -!- FUNCTION: Transcription factor; part of the Fusarium detoxification of
CC benzoxazolinone cluster involved in the degradation of benzoxazolinones
CC produced by the host plant (PubMed:25727347, PubMed:26828593). Maize,
CC wheat, and rye produce the 2 benzoxazinone phytoanticipins 2,4-dihy-
CC droxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA) and 2,4-dihydroxy-1,4-
CC benzoxazin-3-one (DIBOA) that, due to their inherent instability once
CC released, spontaneously degrade to the more stable corresponding
CC benzoxazolinones, 6-methoxy-2-benzoxazolinone (MBOA) and 2-
CC benzoxazolinone (BOA), respectively (By similarity). FDB3 controls the
CC transcription of the FDB gene cluster in response to 6-methoxy-2-
CC benzoxazolinone (MBOA) (PubMed:26828593).
CC {ECO:0000250|UniProtKB:W7MLD5, ECO:0000269|PubMed:25727347,
CC ECO:0000269|PubMed:26828593}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00227}.
CC -!- INDUCTION: Expression is induced in response to 2-benzoxasolinone (BOA)
CC exposure (PubMed:25727347). Expression is also induced in response to
CC 6-methoxy-2-benzoxazolinone (MBOA) and 2-aminophenol (2-AP) treatment
CC (PubMed:26828593). {ECO:0000269|PubMed:25727347,
CC ECO:0000269|PubMed:26828593}.
CC -!- DISRUPTION PHENOTYPE: Reduces significantly the induction of the FBD
CC cluster genes in response to 6-methoxy-2-benzoxazolinone (MBOA)
CC treatment (PubMed:26828593). Impairs the degradation of MBOA but does
CC not affect the degradation of BOA and 2-aminophenol (2-AP)
CC (PubMed:26828593). {ECO:0000269|PubMed:26828593}.
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DR EMBL; AFNW01000283; EKJ71676.1; -; Genomic_DNA.
DR RefSeq; XP_009259515.1; XM_009261240.1.
DR EnsemblFungi; EKJ71676; EKJ71676; FPSE_08122.
DR GeneID; 20366740; -.
DR KEGG; fpu:FPSE_08122; -.
DR eggNOG; ENOG502S0D7; Eukaryota.
DR HOGENOM; CLU_008511_0_0_1; -.
DR InParanoid; K3UIH8; -.
DR Proteomes; UP000007978; Chromosome 2.
DR Proteomes; UP000007978; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR CDD; cd00067; GAL4; 1.
DR Gene3D; 4.10.240.10; -; 1.
DR InterPro; IPR007219; Transcription_factor_dom_fun.
DR InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR Pfam; PF04082; Fungal_trans; 1.
DR Pfam; PF00172; Zn_clus; 1.
DR SMART; SM00906; Fungal_trans; 1.
DR SMART; SM00066; GAL4; 1.
DR SUPFAM; SSF57701; SSF57701; 1.
DR PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE 2: Evidence at transcript level;
KW DNA-binding; Metal-binding; Nucleus; Transcription;
KW Transcription regulation; Zinc.
FT CHAIN 1..757
FT /note="Transcription factor FBD3"
FT /id="PRO_0000454602"
FT DNA_BIND 31..58
FT /note="Zn(2)-C6 fungal-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT REGION 1..24
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 106..161
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 202..222
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..16
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 106..144
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 757 AA; 83355 MW; A37714E36574D89C CRC64;
MSSKRLSQNE QEKSPGTGPP THKRRRIGLA CNACRMRKSR CDGHRPSCSS CLSLGVNCLY
EPGDSTTNVI VRKDYVSDLE QRVASVEHNL QRLNDVLKGH LSPCTGGTDS NNHHNNHDSP
CHQPTIAQSI TSSRPNTRDK SAANETCATG LEEPDDEDAN TNGMAMTFVE EKTSVYYGEA
SNINFTQLLL RALATVHGAT PVAPSASNQA SGPGDGVTTD VPQNQHAVSA SDLATSESSP
TALPSVQEMD SLLDIYFDTA GVVFPFICED TMRRTYNECR HNGFTRARRT WLGTLNMIFA
LASCLSSSAK VRFERCNVFY KRAMALCGEL SKRVVSLEIV HYLILVVLHC QGTQRSIQAW
NNHGLAIRSA MALGLHCQSS GVSLDSAQQE YRRRTWVIIY CLDKVLSTAF GRPASIADEQ
MHSFNGRESV MGLSPMSLSN PQVPVDLPGD FLSVSFRLYQ VMSKSLAEQY GTNLDHVDSG
HDDMAPLKAS GELRKQLRLW AGSLPGHLHV CQPEDEFLAH NTRGNRLRVI LTLRYHNLGI
LIHKPLLSTT IRHLFQAGNR MEAPPSYMIQ LAMAEAHECL RSAQLTIDIV YAVISADPTP
ENNLGAWYFT LYYVFTASLV VSGRLLWAQH GQSIVDEVAV DHCKSLLSKA EEIFKKLDRK
NSLVLSCLEY IARLARICSM KDVPPNHQNG SLDITSDTTN TASGVTDTMP LDEDGMDAFQ
LFAAEMFDPN IFKGFNLSPV EGVSFTDGIW EGFPCGG
