FDC_PSEAE
ID FDC_PSEAE Reviewed; 496 AA.
AC Q9I6N5;
DT 14-OCT-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=UbiD-like decarboxylase {ECO:0000255|HAMAP-Rule:MF_01983, ECO:0000305|PubMed:18591226};
DE EC=4.1.1.- {ECO:0000255|HAMAP-Rule:MF_01983};
DE AltName: Full=Ferulic acid decarboxylase-like protein {ECO:0000255|HAMAP-Rule:MF_01983};
DE AltName: Full=Homologous to UbiD protein A {ECO:0000303|PubMed:18591226};
GN Name=hudA {ECO:0000303|PubMed:18591226}; OrderedLocusNames=PA0254;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
RN [2]
RP INDUCTION.
RX PubMed=18591226; DOI=10.1128/iai.01637-07;
RA Kim S.H., Park S.Y., Heo Y.J., Cho Y.H.;
RT "Drosophila melanogaster-based screening for multihost virulence factors of
RT Pseudomonas aeruginosa PA14 and identification of a virulence-attenuating
RT factor, HudA.";
RL Infect. Immun. 76:4152-4162(2008).
RN [3]
RP X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) IN COMPLEX WITH MANGANESE, AND
RP SUBUNIT.
RX PubMed=23671667; DOI=10.1371/journal.pone.0063161;
RA Jacewicz A., Izumi A., Brunner K., Schnell R., Schneider G.;
RT "Structural insights into the UbiD protein family from the crystal
RT structure of PA0254 from Pseudomonas aeruginosa.";
RL PLoS ONE 8:E63161-E63161(2013).
CC -!- FUNCTION: Catalyzes the reversible decarboxylation of aromatic
CC carboxylic acids (Probable). Attenuates the virulence of P.aeruginosa
CC in a Drosophila model when overexpressed (PubMed:18591226).
CC {ECO:0000255|HAMAP-Rule:MF_01983, ECO:0000269|PubMed:18591226}.
CC -!- COFACTOR:
CC Name=prenyl-FMN; Xref=ChEBI:CHEBI:87746;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01983};
CC Note=Binds 1 prenylated FMN (prenyl-FMN) per subunit.
CC {ECO:0000255|HAMAP-Rule:MF_01983};
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01983,
CC ECO:0000269|PubMed:23671667};
CC -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01983,
CC ECO:0000269|PubMed:23671667}.
CC -!- INDUCTION: Repressed by hudR. {ECO:0000269|PubMed:18591226}.
CC -!- SIMILARITY: Belongs to the UbiD family. UbiD-like/FDC subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_01983, ECO:0000305|PubMed:23671667}.
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DR EMBL; AE004091; AAG03643.1; -; Genomic_DNA.
DR PIR; D83614; D83614.
DR RefSeq; NP_248945.1; NC_002516.2.
DR RefSeq; WP_003115722.1; NZ_QZGE01000024.1.
DR PDB; 4IP2; X-ray; 1.95 A; A/B/C=1-496.
DR PDB; 4IWS; X-ray; 2.30 A; A/B/C/D=1-496.
DR PDB; 7ABN; X-ray; 1.65 A; A/D/H/M=1-496.
DR PDB; 7ABO; X-ray; 1.95 A; A/B/C/D=1-496.
DR PDBsum; 4IP2; -.
DR PDBsum; 4IWS; -.
DR PDBsum; 7ABN; -.
DR PDBsum; 7ABO; -.
DR AlphaFoldDB; Q9I6N5; -.
DR SMR; Q9I6N5; -.
DR STRING; 287.DR97_3211; -.
DR PaxDb; Q9I6N5; -.
DR PRIDE; Q9I6N5; -.
DR EnsemblBacteria; AAG03643; AAG03643; PA0254.
DR GeneID; 881878; -.
DR KEGG; pae:PA0254; -.
DR PATRIC; fig|208964.12.peg.264; -.
DR PseudoCAP; PA0254; -.
DR HOGENOM; CLU_023348_0_0_6; -.
DR InParanoid; Q9I6N5; -.
DR OMA; PPEENHT; -.
DR PhylomeDB; Q9I6N5; -.
DR BioCyc; PAER208964:G1FZ6-256-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0008694; F:3-octaprenyl-4-hydroxybenzoate carboxy-lyase activity; IBA:GO_Central.
DR GO; GO:0016831; F:carboxy-lyase activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR GO; GO:0006744; P:ubiquinone biosynthetic process; IBA:GO_Central.
DR HAMAP; MF_01983; UbiD_FDC; 1.
DR InterPro; IPR002830; UbiD.
DR InterPro; IPR032903; UbiD/Fdc1.
DR PANTHER; PTHR30108; PTHR30108; 1.
DR Pfam; PF01977; UbiD; 1.
DR TIGRFAMs; TIGR00148; TIGR00148; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Aromatic hydrocarbons catabolism; Decarboxylase;
KW Flavoprotein; FMN; Lyase; Manganese; Metal-binding; Reference proteome.
FT CHAIN 1..496
FT /note="UbiD-like decarboxylase"
FT /id="PRO_0000434525"
FT ACT_SITE 278
FT /note="Proton donor"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01983"
FT BINDING 165..170
FT /ligand="prenyl-FMN"
FT /ligand_id="ChEBI:CHEBI:87746"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01983"
FT BINDING 187..188
FT /ligand="prenyl-FMN"
FT /ligand_id="ChEBI:CHEBI:87746"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01983"
FT BINDING 188
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01983,
FT ECO:0000269|PubMed:23671667"
FT BINDING 229
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01983,
FT ECO:0000269|PubMed:23671667"
FT HELIX 2..6
FT /evidence="ECO:0007829|PDB:7ABN"
FT HELIX 8..17
FT /evidence="ECO:0007829|PDB:7ABN"
FT STRAND 21..28
FT /evidence="ECO:0007829|PDB:7ABN"
FT HELIX 33..44
FT /evidence="ECO:0007829|PDB:7ABN"
FT STRAND 48..54
FT /evidence="ECO:0007829|PDB:7ABN"
FT STRAND 62..64
FT /evidence="ECO:0007829|PDB:7ABN"
FT HELIX 74..76
FT /evidence="ECO:0007829|PDB:7ABN"
FT HELIX 79..82
FT /evidence="ECO:0007829|PDB:7ABN"
FT HELIX 83..85
FT /evidence="ECO:0007829|PDB:7ABN"
FT HELIX 93..105
FT /evidence="ECO:0007829|PDB:7ABN"
FT HELIX 118..120
FT /evidence="ECO:0007829|PDB:7ABN"
FT STRAND 121..125
FT /evidence="ECO:0007829|PDB:7ABN"
FT HELIX 126..128
FT /evidence="ECO:0007829|PDB:7ABN"
FT HELIX 131..133
FT /evidence="ECO:0007829|PDB:7ABN"
FT STRAND 147..157
FT /evidence="ECO:0007829|PDB:7ABN"
FT STRAND 164..168
FT /evidence="ECO:0007829|PDB:7ABN"
FT STRAND 171..175
FT /evidence="ECO:0007829|PDB:7ABN"
FT STRAND 178..180
FT /evidence="ECO:0007829|PDB:7ABN"
FT HELIX 188..198
FT /evidence="ECO:0007829|PDB:7ABN"
FT STRAND 203..210
FT /evidence="ECO:0007829|PDB:7ABN"
FT HELIX 213..219
FT /evidence="ECO:0007829|PDB:7ABN"
FT HELIX 229..237
FT /evidence="ECO:0007829|PDB:7ABN"
FT STRAND 242..245
FT /evidence="ECO:0007829|PDB:7ABN"
FT STRAND 247..249
FT /evidence="ECO:0007829|PDB:7ABN"
FT STRAND 252..254
FT /evidence="ECO:0007829|PDB:7ABN"
FT STRAND 258..273
FT /evidence="ECO:0007829|PDB:7ABN"
FT STRAND 281..283
FT /evidence="ECO:0007829|PDB:7ABN"
FT STRAND 288..300
FT /evidence="ECO:0007829|PDB:7ABN"
FT STRAND 305..307
FT /evidence="ECO:0007829|PDB:7ABN"
FT STRAND 312..316
FT /evidence="ECO:0007829|PDB:7ABN"
FT HELIX 317..320
FT /evidence="ECO:0007829|PDB:7ABN"
FT HELIX 322..336
FT /evidence="ECO:0007829|PDB:7ABN"
FT STRAND 341..345
FT /evidence="ECO:0007829|PDB:7ABN"
FT HELIX 348..350
FT /evidence="ECO:0007829|PDB:7ABN"
FT STRAND 353..359
FT /evidence="ECO:0007829|PDB:7ABN"
FT HELIX 361..364
FT /evidence="ECO:0007829|PDB:7ABN"
FT HELIX 365..367
FT /evidence="ECO:0007829|PDB:7ABN"
FT HELIX 371..383
FT /evidence="ECO:0007829|PDB:7ABN"
FT HELIX 388..390
FT /evidence="ECO:0007829|PDB:7ABN"
FT STRAND 393..398
FT /evidence="ECO:0007829|PDB:7ABN"
FT HELIX 406..416
FT /evidence="ECO:0007829|PDB:7ABN"
FT TURN 419..421
FT /evidence="ECO:0007829|PDB:7ABN"
FT STRAND 422..425
FT /evidence="ECO:0007829|PDB:7ABN"
FT HELIX 440..445
FT /evidence="ECO:0007829|PDB:7ABN"
FT STRAND 451..454
FT /evidence="ECO:0007829|PDB:7ABN"
FT HELIX 458..462
FT /evidence="ECO:0007829|PDB:7ABN"
FT STRAND 466..469
FT /evidence="ECO:0007829|PDB:7ABN"
FT TURN 472..474
FT /evidence="ECO:0007829|PDB:7ABN"
FT HELIX 478..491
FT /evidence="ECO:0007829|PDB:7ABN"
FT TURN 492..495
FT /evidence="ECO:0007829|PDB:7ABN"
SQ SEQUENCE 496 AA; 54500 MW; 6C8EB98074A5CD54 CRC64;
MNRSALDFRH FVDHLRRQGD LVDVHTEVDA NLEIGAITRR VYERRAPAPL FHNIRDSLPG
ARVLGAPAGL RADRARAHSR LALHFGLPEH SGPRDIVAML RAAMRAEPIA PRRLERGPVQ
ENVWLGEQVD LTRFPVPLLH EQDGGRYFGT YGFHVVQTPD GSWDSWSVGR LMLVDRNTLA
GPTIPTQHIG IIREQWRRLG KPTPWAMALG APPAALAAAG MPLPEGVSEA GYVGALVGEP
VEVVRTQTNG LWVPANTEIV LEGEISLDET ALEGPMGEYH GYSFPIGKPQ PLFHVHALSF
RDQPILPICV AGTPPEENHT IWGTMISAQL LDVAQNAGLP VDMVWCSYEA ATCWAVLSID
VQRLAALGTD AAAFAARVAE TVFGSHAGHL VPKLILVGND IDVTEIDQVV WALATRAHPL
HDHFAFPQIR DFPMVPYLDA EDKARGSGGR LVINCLYPEQ FAGQMRAATA SFRHAYPTAL
RRRVEERWSD YGFGDA
