ID   FDHB_METFO              Reviewed;         399 AA.
AC   P06130;
DT   01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1988, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Formate dehydrogenase subunit beta;
DE            EC=1.17.1.9;
GN   Name=fdhB;
OS   Methanobacterium formicicum.
OC   Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC   Methanobacteriales; Methanobacteriaceae; Methanobacterium.
OX   NCBI_TaxID=2162;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-22.
RC   STRAIN=JF-1;
RX   PubMed=3531194; DOI=10.1016/s0021-9258(18)69253-1;
RA   Shuber A.P., Orr E.C., Recny M.A., Schendel P.F., May H.D., Schauer N.L.,
RA   Ferry J.G.;
RT   "Cloning, expression, and nucleotide sequence of the formate dehydrogenase
RT   genes from Methanobacterium formicicum.";
RL   J. Biol. Chem. 261:12942-12947(1986).
CC   -!- FUNCTION: Catalyzes the oxidation of formate.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=formate + NAD(+) = CO2 + NADH; Xref=Rhea:RHEA:15985,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:16526, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945; EC=1.17.1.9;
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000305};
CC       Note=Binds 2 [4Fe-4S] clusters. {ECO:0000305};
CC   -!- SUBUNIT: Dimer of an alpha and a beta subunit.
CC   -!- SIMILARITY: Belongs to the FrhB family. {ECO:0000305}.
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DR   EMBL; J02581; AAA72183.1; -; Genomic_DNA.
DR   PIR; B24698; B24698.
DR   RefSeq; WP_023991259.1; NZ_LN734822.1.
DR   AlphaFoldDB; P06130; -.
DR   SMR; P06130; -.
DR   STRING; 2162.BRM9_0167; -.
DR   PRIDE; P06130; -.
DR   GeneID; 26738400; -.
DR   OrthoDB; 34322at2157; -.
DR   SABIO-RK; P06130; -.
DR   GO; GO:0009326; C:formate dehydrogenase complex; IEA:UniProtKB-EC.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0043794; F:formate dehydrogenase (coenzyme F420) activity; IDA:MENGO.
DR   GO; GO:0008863; F:formate dehydrogenase (NAD+) activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.1060.10; -; 1.
DR   InterPro; IPR017896; 4Fe4S_Fe-S-bd.
DR   InterPro; IPR017900; 4Fe4S_Fe_S_CS.
DR   InterPro; IPR007516; Co_F420_Hydgase/DH_bsu_N.
DR   InterPro; IPR045220; FRHB/FDHB/HCAR-like.
DR   InterPro; IPR007525; FrhB_FdhB_C.
DR   InterPro; IPR009051; Helical_ferredxn.
DR   PANTHER; PTHR31332; PTHR31332; 1.
DR   Pfam; PF04432; FrhB_FdhB_C; 1.
DR   Pfam; PF04422; FrhB_FdhB_N; 1.
DR   PROSITE; PS00198; 4FE4S_FER_1; 2.
DR   PROSITE; PS51379; 4FE4S_FER_2; 2.
PE   1: Evidence at protein level;
KW   4Fe-4S; Direct protein sequencing; Electron transport; Iron; Iron-sulfur;
KW   Metal-binding; NAD; Oxidoreductase; Repeat; Transport.
FT   CHAIN           1..399
FT                   /note="Formate dehydrogenase subunit beta"
FT                   /id="PRO_0000159222"
FT   DOMAIN          287..316
FT                   /note="4Fe-4S ferredoxin-type 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00711"
FT   DOMAIN          339..367
FT                   /note="4Fe-4S ferredoxin-type 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00711"
FT   BINDING         296
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         299
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         302
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         306
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         348
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         351
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         354
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         358
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   399 AA;  43922 MW;  D57F778C6C8F67BB CRC64;
     MINTNDMFYA KSSDAEIAEA GEYGGAVTTL LKFLLKEGIV DAVLAVDSSA DLYDVVPILI
     EDPEDVVKAA GSLHFGTLNL AKVVTRYLDG AQDMKIAVTV KPCDAMTMVE LMKREKVNAD
     NVIMVGLNCG GTMPPVKGRQ MMEEFYEVDP DSVVKEEIAK GKLIVETEDG TEKEIPIDEL
     EDEGFGRRTN CRRCEVNIPR MADLACGNWG VIGPLAGKAT FIEVCSPKGA EVLEKAKEAG
     VIDLEDPIPK GIEIREKIDG AMVKLADKWQ GNDWEDKAGR EIFSVLTEYM DDFSRCLKCY
     GCREACPICY CEDCCLEANN GPDWLSKGEI PPSPMFHLER MLHMVESCTN CGQCEEVCPG
     EIPLAKIWHE VNAKMKDTFG YVKGTGDEKP PIAYFPVGK