ID   FDHB_METTF              Reviewed;         394 AA.
AC   Q50570;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=Formate dehydrogenase subunit beta;
DE            EC=1.17.1.9;
GN   Name=fdhB;
OS   Methanothermobacter thermautotrophicus (Methanobacterium thermoformicicum).
OC   Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC   Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
OX   NCBI_TaxID=145262;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=DSM 3720 / Z-245;
RX   PubMed=9006048; DOI=10.1128/jb.179.3.899-908.1997;
RA   Noelling J., Reeve J.N.;
RT   "Growth- and substrate-dependent transcription of the formate dehydrogenase
RT   (fdhCAB) operon in Methanobacterium thermoformicicum Z-245.";
RL   J. Bacteriol. 179:899-908(1997).
CC   -!- FUNCTION: Catalyzes the oxidation of formate.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=formate + NAD(+) = CO2 + NADH; Xref=Rhea:RHEA:15985,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:16526, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945; EC=1.17.1.9;
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000305};
CC       Note=Binds 2 [4Fe-4S] clusters. {ECO:0000305};
CC   -!- SUBUNIT: Dimer of an alpha and a beta subunit.
CC   -!- SIMILARITY: Belongs to the FrhB family. {ECO:0000305}.
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DR   EMBL; U52681; AAC44821.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q50570; -.
DR   SMR; Q50570; -.
DR   GO; GO:0009326; C:formate dehydrogenase complex; IEA:UniProtKB-EC.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0008863; F:formate dehydrogenase (NAD+) activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.1060.10; -; 1.
DR   InterPro; IPR017896; 4Fe4S_Fe-S-bd.
DR   InterPro; IPR017900; 4Fe4S_Fe_S_CS.
DR   InterPro; IPR007516; Co_F420_Hydgase/DH_bsu_N.
DR   InterPro; IPR045220; FRHB/FDHB/HCAR-like.
DR   InterPro; IPR007525; FrhB_FdhB_C.
DR   InterPro; IPR009051; Helical_ferredxn.
DR   PANTHER; PTHR31332; PTHR31332; 1.
DR   Pfam; PF04432; FrhB_FdhB_C; 1.
DR   Pfam; PF04422; FrhB_FdhB_N; 1.
DR   PROSITE; PS00198; 4FE4S_FER_1; 2.
DR   PROSITE; PS51379; 4FE4S_FER_2; 2.
PE   3: Inferred from homology;
KW   4Fe-4S; Electron transport; Iron; Iron-sulfur; Metal-binding; NAD;
KW   Oxidoreductase; Repeat; Transport.
FT   CHAIN           1..394
FT                   /note="Formate dehydrogenase subunit beta"
FT                   /id="PRO_0000159223"
FT   DOMAIN          285..315
FT                   /note="4Fe-4S ferredoxin-type 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00711"
FT   DOMAIN          337..367
FT                   /note="4Fe-4S ferredoxin-type 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00711"
FT   BINDING         295
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         298
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         301
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         305
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         346
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         349
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         352
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         356
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   394 AA;  43683 MW;  F95B3E85B4C44316 CRC64;
     MVEVNDMYYA FSKNEEIAEK GEYGGAVTSL LKFLLEEGMV DAVLAVKKGS DLYDAVPTLI
     TEPEKVIESA GSLHCGTLNI AKVITRYLNG AKDIKIAVTT KPCDAMTIVE VAKRGKIDLD
     NVIMVGVNCG GTLPPVKTRA MIEEVYEMDP DDVVKEEIAK GKLIIETSDA EKGISVDEVE
     EMGYGRRTNC RRCEKNIPRM ADLALGNWGV IGPLAGKATF VEVTSEKGAD LLEKAVSAGV
     IEIEDPIPKG IEIREKIDQA MVNLAKKWQK NDFNEETGAE ILMNLDRYMD ELNKCIKCYS
     CREACPICYC EECSLETKTP EWIEKGKIPP SPLFHLERML HMVDSCTNCG QCEELCPAEI
     PLAKIWHEIN CRVQETFGYK TGFETGQEPP LTHP