ID   FDHS_GLUJA              Reviewed;         183 AA.
AC   M1VB40;
DT   19-FEB-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2013, sequence version 1.
DT   03-AUG-2022, entry version 19.
DE   RecName: Full=Fructose dehydrogenase small subunit;
DE   AltName: Full=Fructose dehydrogenase subunit III;
DE   Flags: Precursor;
GN   Name=fdhS;
OS   Gluconobacter japonicus.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales;
OC   Acetobacteraceae; Gluconobacter.
OX   NCBI_TaxID=376620;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBUNIT, BIOTECHNOLOGY, AND
RP   SUBCELLULAR LOCATION.
RC   STRAIN=ATCC 12302 / IAM 1816 / JCM 20278 / NBRC 3260;
RX   PubMed=23275508; DOI=10.1128/aem.03152-12;
RA   Kawai S., Goda-Tsutsumi M., Yakushi T., Kano K., Matsushita K.;
RT   "Heterologous overexpression and characterization of a flavoprotein-
RT   cytochrome c complex fructose dehydrogenase of Gluconobacter japonicus
RT   NBRC3260.";
RL   Appl. Environ. Microbiol. 79:1654-1660(2013).
RN   [2]
RP   SUBUNIT, AND SUBCELLULAR LOCATION.
RC   STRAIN=ATCC 12302 / IAM 1816 / JCM 20278 / NBRC 3260;
RX   PubMed=7462161; DOI=10.1128/jb.145.2.814-823.1981;
RA   Ameyama M., Shinagawa E., Matsushita K., Adachi O.;
RT   "D-fructose dehydrogenase of Gluconobacter industrius: purification,
RT   characterization, and application to enzymatic microdetermination of D-
RT   fructose.";
RL   J. Bacteriol. 145:814-823(1981).
CC   -!- FUNCTION: Subunit of fructose dehydrogenase, an enzyme that catalyzes
CC       the oxidation of D-fructose to produce 5-keto-D-fructose.
CC       {ECO:0000269|PubMed:23275508}.
CC   -!- SUBUNIT: Heterotrimer composed of FdhL, FdhS and FdhC.
CC       {ECO:0000269|PubMed:23275508, ECO:0000269|PubMed:7462161}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:23275508,
CC       ECO:0000269|PubMed:7462161}.
CC   -!- PTM: Predicted to be exported by the Tat system. The position of the
CC       signal peptide cleavage has not been experimentally proven.
CC   -!- BIOTECHNOLOGY: This enzyme is commonly used in a number of research
CC       projects to examine the electrochemical properties of enzyme-catalyzed
CC       electrode reactions, named bioelectrocatalysis. Available as a
CC       commercial product from Sigma Aldrich (catalog number F4892).
CC       {ECO:0000269|PubMed:23275508}.
CC   -!- SIMILARITY: Belongs to the FAD containing D-sorbitol dehydrogenase
CC       family. {ECO:0000305}.
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DR   EMBL; AB728565; BAM93250.1; -; Genomic_DNA.
DR   AlphaFoldDB; M1VB40; -.
DR   SMR; M1VB40; -.
DR   KEGG; ag:BAM93250; -.
DR   BioCyc; MetaCyc:MON-21742; -.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0006000; P:fructose metabolic process; IDA:UniProtKB.
DR   InterPro; IPR024651; FAD-SLDH_ssu.
DR   Pfam; PF12318; FAD-SLDH; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Membrane; Signal.
FT   SIGNAL          1..45
FT                   /note="Tat-type signal"
FT                   /evidence="ECO:0000255"
FT   CHAIN           46..183
FT                   /note="Fructose dehydrogenase small subunit"
FT                   /id="PRO_0000425560"
SQ   SEQUENCE   183 AA;  20086 MW;  D763F18532ECEC31 CRC64;
     MEKIADSGPV QIFLSRRKLL AFSGASLTVA AIGAPSKGST QDVVASNRDS ISDFMQLSAF
     ATGHKNLDLN IGSALLLAFE AQKHDFSTQI KALREHITKN NYQDVEALDA AMKDDPLHPT
     LIQIIRAWYS GVIEDETNAK VYAFEKALMY QPSRDVVVIP TYAHNGPNYW VSEPASVDVM
     PAF