FECB_ECOLI
ID FECB_ECOLI Reviewed; 300 AA.
AC P15028; P76816; Q2M625;
DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2003, sequence version 3.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=Fe(3+) dicitrate-binding periplasmic protein;
DE AltName: Full=Iron(III) dicitrate-binding periplasmic protein;
DE Flags: Precursor;
GN Name=fecB; OrderedLocusNames=b4290, JW4250;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=2651410; DOI=10.1128/jb.171.5.2626-2633.1989;
RA Staudenmaier H., van Hove B., Yaraghi Z., Braun V.;
RT "Nucleotide sequences of the fecBCDE genes and locations of the proteins
RT suggest a periplasmic-binding-protein-dependent transport mechanism for
RT iron(III) dicitrate in Escherichia coli.";
RL J. Bacteriol. 171:2626-2633(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=7610040; DOI=10.1093/nar/23.12.2105;
RA Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.;
RT "Analysis of the Escherichia coli genome VI: DNA sequence of the region
RT from 92.8 through 100 minutes.";
RL Nucleic Acids Res. 23:2105-2119(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP INDUCTION BY HYDROXYUREA.
RC STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX PubMed=20005847; DOI=10.1016/j.molcel.2009.11.024;
RA Davies B.W., Kohanski M.A., Simmons L.A., Winkler J.A., Collins J.J.,
RA Walker G.C.;
RT "Hydroxyurea induces hydroxyl radical-mediated cell death in Escherichia
RT coli.";
RL Mol. Cell 36:845-860(2009).
CC -!- FUNCTION: Binds citrate-dependent Fe(3+); part of the binding-protein-
CC dependent transport system for uptake of citrate-dependent Fe(3+).
CC -!- SUBCELLULAR LOCATION: Periplasm.
CC -!- INDUCTION: Induced 1.8-fold by hydroxyurea.
CC {ECO:0000269|PubMed:20005847}.
CC -!- SIMILARITY: Belongs to the bacterial solute-binding protein 8 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA97186.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; M26397; AAA23762.1; -; Genomic_DNA.
DR EMBL; U14003; AAA97186.1; ALT_INIT; Genomic_DNA.
DR EMBL; U00096; AAC77246.2; -; Genomic_DNA.
DR EMBL; AP009048; BAE78281.1; -; Genomic_DNA.
DR PIR; S56515; QRECD3.
DR RefSeq; NP_418710.4; NC_000913.3.
DR RefSeq; WP_000879155.1; NZ_LN832404.1.
DR AlphaFoldDB; P15028; -.
DR SMR; P15028; -.
DR BioGRID; 4261562; 224.
DR BioGRID; 851179; 7.
DR ComplexPortal; CPX-4403; Ferric-citrate ABC transporter complex.
DR DIP; DIP-9584N; -.
DR IntAct; P15028; 10.
DR STRING; 511145.b4290; -.
DR TCDB; 3.A.1.14.1; the atp-binding cassette (abc) superfamily.
DR jPOST; P15028; -.
DR PaxDb; P15028; -.
DR PRIDE; P15028; -.
DR EnsemblBacteria; AAC77246; AAC77246; b4290.
DR EnsemblBacteria; BAE78281; BAE78281; BAE78281.
DR GeneID; 946838; -.
DR KEGG; ecj:JW4250; -.
DR KEGG; eco:b4290; -.
DR PATRIC; fig|1411691.4.peg.2409; -.
DR EchoBASE; EB0283; -.
DR eggNOG; COG4594; Bacteria.
DR HOGENOM; CLU_038034_0_0_6; -.
DR InParanoid; P15028; -.
DR OMA; DETWYLG; -.
DR PhylomeDB; P15028; -.
DR BioCyc; EcoCyc:FECB-MON; -.
DR BioCyc; MetaCyc:FECB-MON; -.
DR PRO; PR:P15028; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0055052; C:ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing; IC:ComplexPortal.
DR GO; GO:0016020; C:membrane; IC:ComplexPortal.
DR GO; GO:0030288; C:outer membrane-bounded periplasmic space; ISM:EcoCyc.
DR GO; GO:0033212; P:iron import into cell; IC:ComplexPortal.
DR GO; GO:0055072; P:iron ion homeostasis; IEP:EcoCyc.
DR GO; GO:0010039; P:response to iron ion; IEP:EcoCyc.
DR GO; GO:0033214; P:siderophore-dependent iron import into cell; IMP:EcoCyc.
DR InterPro; IPR002491; ABC_transptr_periplasmic_BD.
DR Pfam; PF01497; Peripla_BP_2; 1.
DR PROSITE; PS50983; FE_B12_PBP; 1.
PE 2: Evidence at transcript level;
KW Ion transport; Iron; Iron transport; Periplasm; Reference proteome; Signal;
KW Transport.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..300
FT /note="Fe(3+) dicitrate-binding periplasmic protein"
FT /id="PRO_0000031820"
FT DOMAIN 39..295
FT /note="Fe/B12 periplasmic-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00344"
FT VARIANT 8
FT /note="L -> V (in strain: B)"
FT CONFLICT 23
FT /note="T -> M (in Ref. 1; AAA23762)"
FT /evidence="ECO:0000305"
FT CONFLICT 57
FT /note="I -> S (in Ref. 1; AAA23762)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 300 AA; 33146 MW; 6024ED0F9C82D0EA CRC64;
MLAFIRFLFA GLLLVISHAF AATVQDEHGT FTLEKTPQRI VVLELSFADA LAAVDVIPIG
IADDNDAKRI LPEVRAHLKP WQSVGTRAQP SLEAIAALKP DLIIADSSRH AGVYIALQQI
APVLLLKSRN ETYAENLQSA AIIGEMVGKK REMQARLEQH KERMAQWASQ LPKGTRVAFG
TSREQQFNLH TQETWTGSVL ASLGLNVPAA MAGASMPSIG LEQLLAVNPA WLLVAHYREE
SIVKRWQQDP LWQMLTAAQK QQVASVDSNT WARMRGIFAA ERIAADTVKI FHHQPLTVVK
