ID   FECI_ECOLI              Reviewed;         173 AA.
AC   P23484; Q2M622;
DT   01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   03-AUG-2022, entry version 159.
DE   RecName: Full=Probable RNA polymerase sigma factor FecI;
GN   Name=fecI; OrderedLocusNames=b4293, JW4253;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RX   PubMed=2254251; DOI=10.1128/jb.172.12.6749-6758.1990;
RA   van Hove B., Staudenmaier H., Braun V.;
RT   "Novel two-component transmembrane transcription control: regulation of
RT   iron dicitrate transport in Escherichia coli K-12.";
RL   J. Bacteriol. 172:6749-6758(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=7610040; DOI=10.1093/nar/23.12.2105;
RA   Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.;
RT   "Analysis of the Escherichia coli genome VI: DNA sequence of the region
RT   from 92.8 through 100 minutes.";
RL   Nucleic Acids Res. 23:2105-2119(1995).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5]
RP   PROTEIN SEQUENCE OF 2-11.
RX   PubMed=8596456; DOI=10.1111/j.1365-2958.1995.mmi_18010163.x;
RA   Angerer A., Enz S., Ochs M., Braun V.;
RT   "Transcriptional regulation of ferric citrate transport in Escherichia coli
RT   K-12. Fecl belongs to a new subfamily of sigma 70-type factors that respond
RT   to extracytoplasmic stimuli.";
RL   Mol. Microbiol. 18:163-174(1995).
RN   [6]
RP   SIMILARITY TO OTHER ECF SIGMA FACTORS.
RX   PubMed=8052622; DOI=10.1073/pnas.91.16.7573;
RA   Lonetto M.A., Brown K.L., Rudd K.E., Buttner M.J.;
RT   "Analysis of the Streptomyces coelicolor sigE gene reveals the existence of
RT   a subfamily of eubacterial RNA polymerase sigma factors involved in the
RT   regulation of extracytoplasmic functions.";
RL   Proc. Natl. Acad. Sci. U.S.A. 91:7573-7577(1994).
RN   [7]
RP   INDUCTION BY HYDROXYUREA.
RC   STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX   PubMed=20005847; DOI=10.1016/j.molcel.2009.11.024;
RA   Davies B.W., Kohanski M.A., Simmons L.A., Winkler J.A., Collins J.J.,
RA   Walker G.C.;
RT   "Hydroxyurea induces hydroxyl radical-mediated cell death in Escherichia
RT   coli.";
RL   Mol. Cell 36:845-860(2009).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. This sigma factor regulates the fec genes for iron dicitrate
CC       transport (Probable). {ECO:0000305}.
CC   -!- INDUCTION: Induced 2.1-fold by hydroxyurea.
CC       {ECO:0000269|PubMed:20005847}.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. ECF subfamily.
CC       {ECO:0000305}.
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DR   EMBL; M63115; AAA23766.1; -; Genomic_DNA.
DR   EMBL; U14003; AAA97189.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC77249.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE78284.1; -; Genomic_DNA.
DR   PIR; JV0111; JV0111.
DR   RefSeq; NP_418713.1; NC_000913.3.
DR   RefSeq; WP_001283626.1; NZ_STEB01000013.1.
DR   AlphaFoldDB; P23484; -.
DR   SMR; P23484; -.
DR   ComplexPortal; CPX-4888; DNA-directed RNA polymerase holoenzyme complex, Sigma fecI variant.
DR   DIP; DIP-9588N; -.
DR   IntAct; P23484; 8.
DR   STRING; 511145.b4293; -.
DR   PaxDb; P23484; -.
DR   PRIDE; P23484; -.
DR   EnsemblBacteria; AAC77249; AAC77249; b4293.
DR   EnsemblBacteria; BAE78284; BAE78284; BAE78284.
DR   GeneID; 58462728; -.
DR   GeneID; 946839; -.
DR   KEGG; ecj:JW4253; -.
DR   KEGG; eco:b4293; -.
DR   PATRIC; fig|1411691.4.peg.2406; -.
DR   EchoBASE; EB0287; -.
DR   eggNOG; COG1595; Bacteria.
DR   HOGENOM; CLU_047691_12_1_6; -.
DR   InParanoid; P23484; -.
DR   OMA; NPRAYLY; -.
DR   PhylomeDB; P23484; -.
DR   BioCyc; EcoCyc:PD00440; -.
DR   BioCyc; MetaCyc:PD00440; -.
DR   PHI-base; PHI:8004; -.
DR   PRO; PR:P23484; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProt.
DR   GO; GO:0032991; C:protein-containing complex; IEA:UniProt.
DR   GO; GO:0001000; F:bacterial-type RNA polymerase core enzyme binding; IDA:EcoCyc.
DR   GO; GO:0001046; F:core promoter sequence-specific DNA binding; IDA:EcoCyc.
DR   GO; GO:0016987; F:sigma factor activity; IDA:EcoCyc.
DR   GO; GO:0006879; P:cellular iron ion homeostasis; IMP:EcoCyc.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IMP:EcoCyc.
DR   GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR   GO; GO:0055072; P:iron ion homeostasis; IC:ComplexPortal.
DR   GO; GO:0006950; P:response to stress; IEA:UniProt.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR039425; RNA_pol_sigma-70-like.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR000838; RNA_pol_sigma70_ECF_CS.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR013249; RNA_pol_sigma70_r4_t2.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   PANTHER; PTHR43133; PTHR43133; 1.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   Pfam; PF08281; Sigma70_r4_2; 1.
DR   SUPFAM; SSF88659; SSF88659; 1.
DR   SUPFAM; SSF88946; SSF88946; 1.
DR   TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR   PROSITE; PS01063; SIGMA70_ECF; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; DNA-binding; Ion transport; Iron;
KW   Iron transport; Reference proteome; Sigma factor; Transcription;
KW   Transcription regulation; Transport.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:8596456"
FT   CHAIN           2..173
FT                   /note="Probable RNA polymerase sigma factor FecI"
FT                   /id="PRO_0000094011"
FT   DNA_BIND        139..158
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   MOTIF           40..52
FT                   /note="Polymerase core binding"
SQ   SEQUENCE   173 AA;  19480 MW;  5CAD4F4840E71FAA CRC64;
     MSDRATTTAS LTFESLYGTH HGWLKSWLTR KLQSAFDADD IAQDTFLRVM VSETLSTIRD
     PRSFLCTIAK RVMVDLFRRN ALEKAYLEML ALMPEGGAPS PEERESQLET LQLLDSMLDG
     LNGKTREAFL LSQLDGLTYS EIAHKLGVSI SSVKKYVAKA VEHCLLFRLE YGL