FEL1B_FELCA
ID FEL1B_FELCA Reviewed; 109 AA.
AC P30440;
DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1993, sequence version 1.
DT 25-MAY-2022, entry version 119.
DE RecName: Full=Major allergen I polypeptide chain 2;
DE AltName: Full=AG4;
DE AltName: Full=Allergen Cat-1;
DE AltName: Full=Allergen Fel d I-B;
DE Short=Allergen FdI;
DE AltName: Allergen=Fel d 1-B;
DE Flags: Precursor;
GN Name=CH2;
OS Felis catus (Cat) (Felis silvestris catus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Carnivora; Feliformia; Felidae; Felinae; Felis.
OX NCBI_TaxID=9685;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 18-100.
RX PubMed=1946388; DOI=10.1073/pnas.88.21.9690;
RA Morgenstern J.P., Griffith I.J., Brauer A.W., Rogers B.L., Bond J.F.,
RA Chapman M.D., Kuo M.-C.;
RT "Amino acid sequence of Fel dI, the major allergen of the domestic cat:
RT protein sequence analysis and cDNA cloning.";
RL Proc. Natl. Acad. Sci. U.S.A. 88:9690-9694(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE, ALTERNATIVE SPLICING, AND VARIANTS.
RC TISSUE=Liver;
RX PubMed=1572548; DOI=10.1016/0378-1119(92)90405-e;
RA Griffith I.J., Craig S., Pollock J., Yu X.-B., Morgenstern J.P.,
RA Rogers B.L.;
RT "Expression and genomic structure of the genes encoding FdI, the major
RT allergen from the domestic cat.";
RL Gene 113:263-268(1992).
RN [3]
RP PROTEIN SEQUENCE OF 18-37, AND CHARACTERIZATION.
RX PubMed=1712068; DOI=10.1016/0161-5890(91)90141-6;
RA Duffort O.A., Carreira J., Nitti G., Polo F., Lombardero M.;
RT "Studies on the biochemical structure of the major cat allergen Felis
RT domesticus I.";
RL Mol. Immunol. 28:301-309(1991).
RN [4]
RP CHARACTERIZATION.
RX PubMed=6747135; DOI=10.1016/0091-6749(84)90278-1;
RA Leitermann K., Ohman J.L. Jr.;
RT "Cat allergen 1: biochemical, antigenic, and allergenic properties.";
RL J. Allergy Clin. Immunol. 74:147-153(1984).
RN [5]
RP X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) OF 18-109.
RX PubMed=12851385; DOI=10.1074/jbc.m304740200;
RA Kaiser L., Gronlund H., Sandalova T., Ljunggren H.G., van Hage-Hamsten M.,
RA Achour A., Schneider G.;
RT "The crystal structure of the major cat allergen Fel d 1, a member of the
RT secretoglobin family.";
RL J. Biol. Chem. 278:37730-37735(2003).
CC -!- SUBUNIT: Heterotetramer composed of two non-covalently linked
CC disulfide-linked heterodimer of chains 1 and 2.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Comment=Experimental confirmation may be lacking for some isoforms.;
CC Name=1; Synonyms=CH2L;
CC IsoId=P30440-1; Sequence=Displayed;
CC Name=2; Synonyms=CH2S;
CC IsoId=P30440-2; Sequence=VSP_004249;
CC Name=3; Synonyms=CH2ST, Truncated;
CC IsoId=P30440-3; Sequence=VSP_004248;
CC -!- TISSUE SPECIFICITY: The long form is preferentially expressed in the
CC salivary gland, while the short form is preferentially expressed in the
CC skin.
CC -!- ALLERGEN: Causes an allergic reaction in human. Binds to IgE. Major
CC allergen produced by the domestic cat. Implicated as an asthma-inducing
CC agent in human. This protein is sticky and easily adheres to walls,
CC carpet, clothing, furniture and bedding.
CC -!- SIMILARITY: Belongs to the secretoglobin family. {ECO:0000305}.
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DR EMBL; M77341; AAC41616.1; -; mRNA.
DR EMBL; X62478; CAA44345.1; -; Genomic_DNA.
DR PIR; B53283; B53283.
DR PIR; C56413; C56413.
DR PIR; JC1127; JC1127.
DR RefSeq; NP_001041619.1; NM_001048154.1. [P30440-1]
DR PDB; 1PUO; X-ray; 1.85 A; A/B=18-109.
DR PDB; 1ZKR; X-ray; 1.64 A; A/B=18-93.
DR PDB; 2EJN; X-ray; 1.64 A; A/B=18-93.
DR PDB; 5VYF; X-ray; 2.90 A; C/F=18-109.
DR PDBsum; 1PUO; -.
DR PDBsum; 1ZKR; -.
DR PDBsum; 2EJN; -.
DR PDBsum; 5VYF; -.
DR AlphaFoldDB; P30440; -.
DR SMR; P30440; -.
DR STRING; 9685.ENSFCAP00000019678; -.
DR Allergome; 3278; Fel d 1.0101.
DR Allergome; 345; Fel d 1.
DR ABCD; P30440; 4 sequenced antibodies.
DR GeneID; 677879; -.
DR CTD; 677879; -.
DR eggNOG; ENOG502RU0W; Eukaryota.
DR InParanoid; P30440; -.
DR EvolutionaryTrace; P30440; -.
DR Proteomes; UP000011712; Unplaced.
DR GO; GO:0005615; C:extracellular space; IEA:InterPro.
DR CDD; cd00633; Secretoglobin; 1.
DR InterPro; IPR015332; CH2-like.
DR InterPro; IPR016126; Secretoglobin.
DR InterPro; IPR035960; Secretoglobin_sf.
DR PANTHER; PTHR31708; PTHR31708; 1.
DR Pfam; PF09252; Feld-I_B; 1.
DR SUPFAM; SSF48201; SSF48201; 1.
DR PROSITE; PS51311; SCGB; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Allergen; Alternative splicing; Direct protein sequencing;
KW Disulfide bond; Glycoprotein; Reference proteome; Secreted; Signal.
FT SIGNAL 1..17
FT /evidence="ECO:0000269|PubMed:1712068,
FT ECO:0000269|PubMed:1946388"
FT CHAIN 18..109
FT /note="Major allergen I polypeptide chain 2"
FT /id="PRO_0000021248"
FT CARBOHYD 50
FT /note="N-linked (GlcNAc...) asparagine"
FT DISULFID 24
FT /note="Interchain (with C-92 in chain 1)"
FT DISULFID 65
FT /note="Interchain (with C-66 in chain 1)"
FT DISULFID 90
FT /note="Interchain (with C-25 in chain 1)"
FT VAR_SEQ 82..109
FT /note="TTISSSKDCMGEAVQNTVEDLKLNTLGR -> PSTNIAWVKQFRTP (in
FT isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_004248"
FT VAR_SEQ 82..89
FT /note="TTISSSKD -> IAINEY (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_004249"
FT VARIANT 72
FT /note="I -> L (in CH2LV)"
FT VARIANT 72
FT /note="I -> V (in CH2SV)"
FT VARIANT 74..75
FT /note="RV -> KF (in CH2SV)"
FT VARIANT 91
FT /note="M -> T (in CH2LV)"
FT VARIANT 96
FT /note="Q -> E (in CH2SV)"
FT VARIANT 105
FT /note="N -> K (in CH2SV)"
FT CONFLICT 24
FT /note="C -> F (in Ref. 3; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 32
FT /note="F -> T (in Ref. 3; AA sequence)"
FT /evidence="ECO:0000305"
FT STRAND 19..23
FT /evidence="ECO:0007829|PDB:1ZKR"
FT HELIX 25..35
FT /evidence="ECO:0007829|PDB:1ZKR"
FT HELIX 39..48
FT /evidence="ECO:0007829|PDB:1ZKR"
FT HELIX 53..68
FT /evidence="ECO:0007829|PDB:1ZKR"
FT HELIX 71..73
FT /evidence="ECO:0007829|PDB:1ZKR"
FT STRAND 74..76
FT /evidence="ECO:0007829|PDB:1ZKR"
FT HELIX 77..85
FT /evidence="ECO:0007829|PDB:1ZKR"
FT TURN 88..90
FT /evidence="ECO:0007829|PDB:1ZKR"
SQ SEQUENCE 109 AA; 11854 MW; 857FB9CD76036CB9 CRC64;
MRGALLVLAL LVTQALGVKM AETCPIFYDV FFAVANGNEL LLDLSLTKVN ATEPERTAMK
KIQDCYVENG LISRVLDGLV MTTISSSKDC MGEAVQNTVE DLKLNTLGR
