FEM1A_DANRE
ID FEM1A_DANRE Reviewed; 617 AA.
AC Q6P9Z4;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=Protein fem-1 homolog A {ECO:0000305};
DE Short=FEM1a {ECO:0000305};
DE AltName: Full=FEM1-alpha {ECO:0000305};
GN Name=fem1a {ECO:0000250|UniProtKB:Q9BSK4};
GN ORFNames=zgc:63483 {ECO:0000303|Ref.1};
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=AB;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Substrate-recognition component of a Cul2-RING (CRL2) E3
CC ubiquitin-protein ligase complex of the DesCEND (destruction via C-end
CC degrons) pathway, which recognizes a C-degron located at the extreme C
CC terminus of target proteins, leading to their ubiquitination and
CC degradation. The C-degron recognized by the DesCEND pathway is usually
CC a motif of less than ten residues and can be present in full-length
CC proteins, truncated proteins or proteolytically cleaved forms. The
CC CRL2(FEM1A) complex specifically recognizes proteins with an arginine
CC at the C-terminus: recognizes and binds proteins ending with -Lys/Arg-
CC Xaa-Arg and -Lys/Arg-Xaa-Xaa-Arg C-degrons, leading to their
CC ubiquitination and degradation. {ECO:0000250|UniProtKB:Q9BSK4}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000250|UniProtKB:Q9BSK4}.
CC -!- SUBUNIT: Component of a CRL2 E3 ubiquitin-protein ligase complex, also
CC named ECS (Elongin BC-CUL2/5-SOCS-box protein) complex.
CC {ECO:0000250|UniProtKB:Q9BSK4}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250|UniProtKB:Q9BSK4}.
CC Cytoplasm {ECO:0000250|UniProtKB:Q9BSK4}.
CC -!- SIMILARITY: Belongs to the fem-1 family. {ECO:0000305}.
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DR EMBL; BC060522; AAH60522.1; -; mRNA.
DR RefSeq; NP_956131.1; NM_199837.1.
DR AlphaFoldDB; Q6P9Z4; -.
DR SMR; Q6P9Z4; -.
DR STRING; 7955.ENSDARP00000110111; -.
DR PaxDb; Q6P9Z4; -.
DR GeneID; 327613; -.
DR KEGG; dre:327613; -.
DR CTD; 55527; -.
DR ZFIN; ZDB-GENE-030131-5824; fem1a.
DR eggNOG; KOG0508; Eukaryota.
DR InParanoid; Q6P9Z4; -.
DR OrthoDB; 252380at2759; -.
DR PhylomeDB; Q6P9Z4; -.
DR Reactome; R-DRE-8951664; Neddylation.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q6P9Z4; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Unplaced.
DR GO; GO:0031462; C:Cul2-RING ubiquitin ligase complex; ISS:UniProtKB.
DR GO; GO:0005739; C:mitochondrion; ISS:UniProtKB.
DR GO; GO:0000151; C:ubiquitin ligase complex; IBA:GO_Central.
DR GO; GO:1990756; F:ubiquitin ligase-substrate adaptor activity; ISS:UniProtKB.
DR GO; GO:0050728; P:negative regulation of inflammatory response; IBA:GO_Central.
DR GO; GO:0050729; P:positive regulation of inflammatory response; ISS:UniProtKB.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR GO; GO:0051438; P:regulation of ubiquitin-protein transferase activity; ISS:UniProtKB.
DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR GO; GO:0140627; P:ubiquitin-dependent protein catabolic process via the C-end degron rule pathway; ISS:UniProtKB.
DR Gene3D; 1.25.40.10; -; 1.
DR Gene3D; 1.25.40.20; -; 3.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR Pfam; PF12796; Ank_2; 2.
DR Pfam; PF13606; Ank_3; 1.
DR Pfam; PF13857; Ank_5; 1.
DR PRINTS; PR01415; ANKYRIN.
DR SMART; SM00248; ANK; 8.
DR SUPFAM; SSF48403; SSF48403; 2.
DR PROSITE; PS50297; ANK_REP_REGION; 2.
DR PROSITE; PS50088; ANK_REPEAT; 7.
PE 2: Evidence at transcript level;
KW ANK repeat; Cytoplasm; Mitochondrion; Reference proteome; Repeat;
KW TPR repeat; Ubl conjugation pathway.
FT CHAIN 1..617
FT /note="Protein fem-1 homolog A"
FT /id="PRO_0000324529"
FT REPEAT 2..32
FT /note="ANK 1"
FT REPEAT 40..70
FT /note="ANK 2"
FT REPEAT 82..111
FT /note="ANK 3"
FT REPEAT 115..144
FT /note="ANK 4"
FT REPEAT 148..177
FT /note="ANK 5"
FT REPEAT 181..210
FT /note="ANK 6"
FT REPEAT 213..242
FT /note="ANK 7"
FT REPEAT 245..279
FT /note="TPR 1"
FT REPEAT 339..372
FT /note="TPR 2"
FT REPEAT 482..524
FT /note="ANK 8"
FT REPEAT 528..557
FT /note="ANK 9"
SQ SEQUENCE 617 AA; 68011 MW; 40C5523DE8BA84BA CRC64;
MDISAAVFNA ARDGKLKLMQ KLLINKSPEE RAALAEERTE GGTPLLIAAR YGHLPVVHFL
LERCGANVAL GGSVNFDGET IEGAPPLWAA SAAGHLPVVK ALLEHGAPVN NTTLTNSTPL
RAACFDGHLE IVRYLVEHQA DLEVANRHGH TCLMISCYKG HREIAQFLLE KGADVNRKSV
KGNTALHDCA ESGSLEIMKM LLKCDARMER DGYGMTPLLA ASVTGHTNIV EFLVHQPRAS
REQRIHALEL LGATFVDKKR DLLGAMRYWR RAMELRWAGG QAGALEKPTA GPLVPAYDCS
REVSTAEELE ALITDPDDMR MQALLVRERI LGPAHPDTSY YIRYRGAVYA DSGNFERCIR
LWKYALDMQQ SNLEPLSPMT ASSFLSFAEL FSFVLQDRAK GTLAARVSFQ DLMGVLSKSV
REVERAVAQR ERPPEPPQFS KALSIILHLL FLLQKLRCGP EQEHLKRQTV YRLLKLNPRA
RGGHTPLHMA VDRDTTSVGR YPVGRFPSLA VASLLLECGA DVDSRDYDNN TPLHIAAANG
CPDIMAALIR AGAHFDATNA AQQTAYQLLE AQSSGRHALH PLNHTTLQCL AARAVTAHRL
PYKGLISEQM EAFIELH
