ID   FEM1A_DROME             Reviewed;         664 AA.
AC   Q9VFD5; Q8IG97; Q8T4I2;
DT   18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT   04-DEC-2007, sequence version 2.
DT   03-AUG-2022, entry version 160.
DE   RecName: Full=Protein fem-1 homolog CG6966;
GN   ORFNames=CG6966;
OS   Drosophila melanogaster (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7227;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Berkeley;
RX   PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA   Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA   Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA   George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA   Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA   Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA   Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA   An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA   Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA   Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA   Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA   Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA   Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA   Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA   Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA   Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA   Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA   Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA   Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA   Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA   Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA   Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA   McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA   Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA   Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA   Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA   Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA   Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA   Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA   Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA   Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA   Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA   Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA   Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA   Venter J.C.;
RT   "The genome sequence of Drosophila melanogaster.";
RL   Science 287:2185-2195(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=Berkeley;
RX   PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA   Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA   Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA   Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA   Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA   Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA   Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT   "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT   review.";
RL   Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Berkeley; TISSUE=Embryo, and Testis;
RX   PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA   Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA   Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA   Celniker S.E.;
RT   "A Drosophila full-length cDNA resource.";
RL   Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC   -!- FUNCTION: Substrate-recognition component of a Cul2-RING (CRL2) E3
CC       ubiquitin-protein ligase complex of the DesCEND (destruction via C-end
CC       degrons) pathway, which recognizes a C-degron located at the extreme C
CC       terminus of target proteins, leading to their ubiquitination and
CC       degradation. The C-degron recognized by the DesCEND pathway is usually
CC       a motif of less than ten residues and can be present in full-length
CC       proteins, truncated proteins or proteolytically cleaved forms.
CC       {ECO:0000250|UniProtKB:Q9BSK4}.
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC       {ECO:0000250|UniProtKB:Q9BSK4}.
CC   -!- SUBUNIT: Component of a CRL2 E3 ubiquitin-protein ligase complex, also
CC       named ECS (Elongin BC-CUL2/5-SOCS-box protein) complex.
CC       {ECO:0000250|UniProtKB:Q9BSK4}.
CC   -!- SIMILARITY: Belongs to the fem-1 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAL89839.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AE014297; AAF55125.2; -; Genomic_DNA.
DR   EMBL; AY084101; AAL89839.1; ALT_INIT; mRNA.
DR   EMBL; BT001887; AAN71661.1; -; mRNA.
DR   RefSeq; NP_001097796.1; NM_001104326.2.
DR   RefSeq; NP_001287333.1; NM_001300404.1.
DR   AlphaFoldDB; Q9VFD5; -.
DR   SMR; Q9VFD5; -.
DR   BioGRID; 66882; 1.
DR   IntAct; Q9VFD5; 1.
DR   STRING; 7227.FBpp0112148; -.
DR   PaxDb; Q9VFD5; -.
DR   DNASU; 41816; -.
DR   EnsemblMetazoa; FBtr0113236; FBpp0112148; FBgn0038286.
DR   EnsemblMetazoa; FBtr0344380; FBpp0310753; FBgn0038286.
DR   GeneID; 41816; -.
DR   KEGG; dme:Dmel_CG6966; -.
DR   UCSC; CG6966-RB; d. melanogaster.
DR   FlyBase; FBgn0038286; CG6966.
DR   VEuPathDB; VectorBase:FBgn0038286; -.
DR   eggNOG; KOG0508; Eukaryota.
DR   GeneTree; ENSGT00940000161115; -.
DR   HOGENOM; CLU_020042_1_0_1; -.
DR   InParanoid; Q9VFD5; -.
DR   OMA; KQTQCPP; -.
DR   OrthoDB; 252380at2759; -.
DR   PhylomeDB; Q9VFD5; -.
DR   Reactome; R-DME-8951664; Neddylation.
DR   UniPathway; UPA00143; -.
DR   BioGRID-ORCS; 41816; 0 hits in 3 CRISPR screens.
DR   ChiTaRS; CG6966; fly.
DR   GenomeRNAi; 41816; -.
DR   PRO; PR:Q9VFD5; -.
DR   Proteomes; UP000000803; Chromosome 3R.
DR   Bgee; FBgn0038286; Expressed in crop (Drosophila) and 26 other tissues.
DR   ExpressionAtlas; Q9VFD5; baseline and differential.
DR   Genevisible; Q9VFD5; DM.
DR   GO; GO:0000151; C:ubiquitin ligase complex; IBA:GO_Central.
DR   GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR   GO; GO:0051438; P:regulation of ubiquitin-protein transferase activity; ISS:UniProtKB.
DR   GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR   Gene3D; 1.25.40.10; -; 1.
DR   Gene3D; 1.25.40.20; -; 3.
DR   InterPro; IPR002110; Ankyrin_rpt.
DR   InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR   InterPro; IPR011990; TPR-like_helical_dom_sf.
DR   Pfam; PF12796; Ank_2; 1.
DR   Pfam; PF13606; Ank_3; 1.
DR   Pfam; PF13637; Ank_4; 2.
DR   PRINTS; PR01415; ANKYRIN.
DR   SMART; SM00248; ANK; 8.
DR   SUPFAM; SSF48403; SSF48403; 2.
DR   PROSITE; PS50297; ANK_REP_REGION; 2.
DR   PROSITE; PS50088; ANK_REPEAT; 6.
PE   2: Evidence at transcript level;
KW   ANK repeat; Reference proteome; Repeat; TPR repeat;
KW   Ubl conjugation pathway.
FT   CHAIN           1..664
FT                   /note="Protein fem-1 homolog CG6966"
FT                   /id="PRO_0000324540"
FT   REPEAT          40..70
FT                   /note="ANK 1"
FT   REPEAT          82..111
FT                   /note="ANK 2"
FT   REPEAT          115..144
FT                   /note="ANK 3"
FT   REPEAT          148..177
FT                   /note="ANK 4"
FT   REPEAT          181..210
FT                   /note="ANK 5"
FT   REPEAT          213..242
FT                   /note="ANK 6"
FT   REPEAT          245..279
FT                   /note="TPR 1"
FT   REPEAT          335..368
FT                   /note="TPR 2"
FT   REPEAT          529..571
FT                   /note="ANK 7"
FT   REPEAT          575..605
FT                   /note="ANK 8"
FT   REGION          433..460
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   664 AA;  73786 MW;  470E17BFC12E3E00 CRC64;
     MDYKFIVFNA ARDNNLAQLK ATLYNKSSVE VGSLISAKVN GATPLVISCR NGHYDIVEYL
     LTKCRANVEQ VGSVSFDGEP IEDAPPLWCA AAAGHLGIVK MLVRRGANVN STTRTNSTPL
     RAACFDGHYE IVKYLVHHGA DFEVANRHGH TCLMIACYKG HFRIAQYLLS LNADVNRCSV
     KGNTALHDCA ESGSLQILQL LLKHGATMDV DYYGMTPLLA ASVTGHMPIV EHLITLPCVS
     RESRIHALEL LGATYVDRKR DMAAALNLWR RALEERAVEP PLEKKVQEPV PAYEMVREVT
     SVEELEEMVL DPDEMRMQAL VIRQRILGPT HPDTSYYIRF RGAHYADAGR FDRCIELWSY
     ALTMQQKILQ PLSPMTQSSL LSFAELFSFM LVEAGRLLPR GRVVPPIEPD SMLTIFYKAV
     KEVERGLAFT LEQQKDQQHP QKQLPAADKS PSCSASSSAS SSSSTTLLSA HQHDCNHDPN
     ALSRTMISAI HIGCLLSSLL DTDALSPEMR RQVMGALYRL NRLKVRVRFD RTALHYACYR
     EGTLAGRYPS CQFPSVTLAK ALLEVGADPN AIDEAGNTPL HLATMQPYVE PLSHILLEGG
     AHLDTKNYAG ETFESLLAPT PMHKIIDPMK YTTLACLAAR TIKKHDIRYE GTVPATLYEF
     IELH