FEM1B_DROME
ID FEM1B_DROME Reviewed; 650 AA.
AC A1ZBY1; A1ZBY0; Q8IGM5; Q8MS72;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 06-FEB-2007, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Protein fem-1 homolog B;
DE AltName: Full=dFEM-1;
GN Name=Fem-1; ORFNames=CG9025;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS A AND B).
RC STRAIN=Berkeley; TISSUE=Embryo, Larva, and Pupae;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP IDENTIFICATION, AND TISSUE SPECIFICITY.
RX PubMed=16415344; DOI=10.1074/jbc.m512468200;
RA Montana E.S., Littleton J.T.;
RT "Expression profiling of a hypercontraction-induced myopathy in Drosophila
RT suggests a compensatory cytoskeletal remodeling response.";
RL J. Biol. Chem. 281:8100-8109(2006).
CC -!- FUNCTION: Substrate-recognition component of a Cul2-RING (CRL2) E3
CC ubiquitin-protein ligase complex of the DesCEND (destruction via C-end
CC degrons) pathway, which recognizes a C-degron located at the extreme C
CC terminus of target proteins, leading to their ubiquitination and
CC degradation. The C-degron recognized by the DesCEND pathway is usually
CC a motif of less than ten residues and can be present in full-length
CC proteins, truncated proteins or proteolytically cleaved forms.
CC {ECO:0000250|UniProtKB:Q9BSK4}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000250|UniProtKB:Q9BSK4}.
CC -!- SUBUNIT: Component of a CRL2 E3 ubiquitin-protein ligase complex, also
CC named ECS (Elongin BC-CUL2/5-SOCS-box protein) complex.
CC {ECO:0000250|UniProtKB:Q9BSK4}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=B;
CC IsoId=A1ZBY1-1; Sequence=Displayed;
CC Name=A;
CC IsoId=A1ZBY1-3; Sequence=VSP_032268;
CC -!- TISSUE SPECIFICITY: Primarily detected in the embryonic central nervous
CC system. {ECO:0000269|PubMed:16415344}.
CC -!- SIMILARITY: Belongs to the fem-1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM50903.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=AAN71457.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AE013599; AAF57431.2; -; Genomic_DNA.
DR EMBL; AE013599; AAM68397.1; -; Genomic_DNA.
DR EMBL; AY119043; AAM50903.1; ALT_FRAME; mRNA.
DR EMBL; BT001702; AAN71457.1; ALT_INIT; mRNA.
DR RefSeq; NP_611508.1; NM_137664.3. [A1ZBY1-3]
DR RefSeq; NP_725993.1; NM_166408.2. [A1ZBY1-1]
DR AlphaFoldDB; A1ZBY1; -.
DR SMR; A1ZBY1; -.
DR BioGRID; 62993; 25.
DR STRING; 7227.FBpp0085558; -.
DR PaxDb; A1ZBY1; -.
DR PRIDE; A1ZBY1; -.
DR DNASU; 37344; -.
DR EnsemblMetazoa; FBtr0086245; FBpp0085558; FBgn0034542. [A1ZBY1-3]
DR EnsemblMetazoa; FBtr0086246; FBpp0085559; FBgn0034542. [A1ZBY1-1]
DR GeneID; 37344; -.
DR KEGG; dme:Dmel_CG9025; -.
DR UCSC; CG9025-RA; d. melanogaster. [A1ZBY1-1]
DR CTD; 37344; -.
DR FlyBase; FBgn0034542; Fem-1.
DR VEuPathDB; VectorBase:FBgn0034542; -.
DR eggNOG; KOG0508; Eukaryota.
DR GeneTree; ENSGT00940000161115; -.
DR HOGENOM; CLU_020042_1_0_1; -.
DR InParanoid; A1ZBY1; -.
DR OMA; NKHIYDL; -.
DR PhylomeDB; A1ZBY1; -.
DR Reactome; R-DME-8951664; Neddylation.
DR UniPathway; UPA00143; -.
DR BioGRID-ORCS; 37344; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 37344; -.
DR PRO; PR:A1ZBY1; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0034542; Expressed in crop (Drosophila) and 24 other tissues.
DR ExpressionAtlas; A1ZBY1; baseline and differential.
DR Genevisible; A1ZBY1; DM.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR GO; GO:0051438; P:regulation of ubiquitin-protein transferase activity; ISS:UniProtKB.
DR Gene3D; 1.25.40.20; -; 3.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR Pfam; PF12796; Ank_2; 2.
DR PRINTS; PR01415; ANKYRIN.
DR SMART; SM00248; ANK; 7.
DR SUPFAM; SSF48403; SSF48403; 2.
DR PROSITE; PS50297; ANK_REP_REGION; 2.
DR PROSITE; PS50088; ANK_REPEAT; 3.
PE 2: Evidence at transcript level;
KW Alternative splicing; ANK repeat; Reference proteome; Repeat;
KW Ubl conjugation pathway.
FT CHAIN 1..650
FT /note="Protein fem-1 homolog B"
FT /id="PRO_0000324541"
FT REPEAT 48..80
FT /note="ANK 1"
FT REPEAT 90..122
FT /note="ANK 2"
FT REPEAT 123..155
FT /note="ANK 3"
FT REPEAT 156..188
FT /note="ANK 4"
FT REPEAT 189..221
FT /note="ANK 5"
FT REPEAT 222..244
FT /note="ANK 6"
FT REPEAT 496..543
FT /note="ANK 7"
FT VAR_SEQ 1..40
FT /note="MHAELYRTRKRWYESAWPGAGFWRGANDTLPVTNYITYPA -> MDVDPAEE
FT APLDEAHTSIYDGYPPDFGYFNMAHGMLVEISEEIRAKFPHEPRLRFSQLNNLHLAARS
FT GQMLSFVEILNSVSCRAVQTHLVNT (in isoform A)"
FT /evidence="ECO:0000303|PubMed:12537569"
FT /id="VSP_032268"
SQ SEQUENCE 650 AA; 73048 MW; 20CF39D6EC4825C3 CRC64;
MHAELYRTRK RWYESAWPGA GFWRGANDTL PVTNYITYPA NFDQPDGQSL TPLTMAAMMG
NVKFVKTLLS HYDVDLDREC NVIYDGMVVY GATALWVAAG MGHLQIVKML VQAGAAINHN
TKAQSSPLRA ACYEGRLDIV EFLIDNGADV NATNLFNNNT LMIAAYKGHH LVVKTLLQNG
SRANDQALCG ATALHYAAES GHLDVVVALL DHGATLKKNE LGITPALQAA ERLHEDVLEA
FIERPELMSL EEQITALELL GATYANDKIK YDVNKAYGYL LRAMQLRYSN PRGVIRKRVQ
PTVPAYDNWL ETENLPELYA IKLNHHSIHM ESLAIRERIL GRNNPELPQA IIYRGAVMAD
QGRFYQCQVL WNYAIDLRMR NNVSVDRDLL RFAQLFAQIL RVENHNLTLD HVLPVLAKCQ
QEIENNKFKI KEAKPNACPS LWQDQNNQNC ITALYLIKIV THLARRKKDQ NIDEEHIQQL
FLVVRKFIQN DTRLQDGQTL LHIAVNGVMP VDEFYTNEMC RFPCYATALV LVHCGASVVA
VDSDRNTPLH ILVTKVNTTQ DRQAEMARIL ELFVEAGAHL DAVNAAGQTA ATACKLPILA
NRLHAHQNAH TSLKCLAARS IATNRLNFKG LIPTQLEAFI QMHSVRKVLP
