ID   FEM1C_XENLA             Reviewed;         617 AA.
AC   Q2T9K6;
DT   18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-JAN-2006, sequence version 1.
DT   03-AUG-2022, entry version 76.
DE   RecName: Full=Protein fem-1 homolog C {ECO:0000305};
DE            Short=FEM1c {ECO:0000250|UniProtKB:Q96JP0};
DE   AltName: Full=FEM1-gamma;
GN   Name=fem1c {ECO:0000250|UniProtKB:Q96JP0};
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Substrate-recognition component of a Cul2-RING (CRL2) E3
CC       ubiquitin-protein ligase complex of the DesCEND (destruction via C-end
CC       degrons) pathway, which recognizes a C-degron located at the extreme C
CC       terminus of target proteins, leading to their ubiquitination and
CC       degradation. The C-degron recognized by the DesCEND pathway is usually
CC       a motif of less than ten residues and can be present in full-length
CC       proteins, truncated proteins or proteolytically cleaved forms. The
CC       CRL2(FEM1C) complex specifically recognizes proteins with an arginine
CC       at the C-terminus: recognizes and binds proteins ending with -Lys/Arg-
CC       Xaa-Arg and -Lys/Arg-Xaa-Xaa-Arg C-degrons, leading to their
CC       ubiquitination and degradation. {ECO:0000250|UniProtKB:Q96JP0}.
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC       {ECO:0000250|UniProtKB:Q96JP0}.
CC   -!- SUBUNIT: Component of a CRL2 E3 ubiquitin-protein ligase complex, also
CC       named ECS (Elongin BC-CUL2/5-SOCS-box protein) complex.
CC       {ECO:0000250|UniProtKB:Q96JP0}.
CC   -!- DOMAIN: The first seven ANK repeats at the N-terminus (1-242) are
CC       essnetial for recognition of Lys/Arg-Xaa-Arg and -Lys/Arg-Xaa-Xaa-Arg
CC       C-degrons. {ECO:0000250|UniProtKB:Q96JP0}.
CC   -!- SIMILARITY: Belongs to the fem-1 family. {ECO:0000305}.
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DR   EMBL; BC111474; AAI11475.1; -; mRNA.
DR   RefSeq; NP_001090163.1; NM_001096694.1.
DR   RefSeq; XP_018106323.1; XM_018250834.1.
DR   AlphaFoldDB; Q2T9K6; -.
DR   SMR; Q2T9K6; -.
DR   PRIDE; Q2T9K6; -.
DR   DNASU; 735243; -.
DR   GeneID; 735243; -.
DR   KEGG; xla:735243; -.
DR   CTD; 735243; -.
DR   Xenbase; XB-GENE-998532; fem1c.L.
DR   OMA; KQTQCPP; -.
DR   OrthoDB; 252380at2759; -.
DR   UniPathway; UPA00143; -.
DR   Proteomes; UP000186698; Chromosome 1L.
DR   Bgee; 735243; Expressed in ovary and 19 other tissues.
DR   GO; GO:0031462; C:Cul2-RING ubiquitin ligase complex; ISS:UniProtKB.
DR   GO; GO:1990756; F:ubiquitin ligase-substrate adaptor activity; ISS:UniProtKB.
DR   GO; GO:0043161; P:proteasome-mediated ubiquitin-dependent protein catabolic process; ISS:UniProtKB.
DR   GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR   GO; GO:0140627; P:ubiquitin-dependent protein catabolic process via the C-end degron rule pathway; ISS:UniProtKB.
DR   Gene3D; 1.25.40.10; -; 1.
DR   Gene3D; 1.25.40.20; -; 3.
DR   InterPro; IPR002110; Ankyrin_rpt.
DR   InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR   InterPro; IPR011990; TPR-like_helical_dom_sf.
DR   Pfam; PF12796; Ank_2; 3.
DR   Pfam; PF13857; Ank_5; 1.
DR   PRINTS; PR01415; ANKYRIN.
DR   SMART; SM00248; ANK; 9.
DR   SUPFAM; SSF48403; SSF48403; 2.
DR   SUPFAM; SSF48452; SSF48452; 1.
DR   PROSITE; PS50297; ANK_REP_REGION; 2.
DR   PROSITE; PS50088; ANK_REPEAT; 7.
PE   2: Evidence at transcript level;
KW   ANK repeat; Reference proteome; Repeat; TPR repeat;
KW   Ubl conjugation pathway.
FT   CHAIN           1..617
FT                   /note="Protein fem-1 homolog C"
FT                   /id="PRO_0000324539"
FT   REPEAT          2..31
FT                   /note="ANK 1"
FT   REPEAT          40..70
FT                   /note="ANK 2"
FT   REPEAT          82..111
FT                   /note="ANK 3"
FT   REPEAT          115..144
FT                   /note="ANK 4"
FT   REPEAT          148..177
FT                   /note="ANK 5"
FT   REPEAT          181..210
FT                   /note="ANK 6"
FT   REPEAT          213..242
FT                   /note="ANK 7"
FT   REPEAT          245..279
FT                   /note="TPR 1"
FT   REPEAT          338..371
FT                   /note="TPR 2"
FT   REPEAT          481..523
FT                   /note="ANK 8"
FT   REPEAT          527..556
FT                   /note="ANK 9"
SQ   SEQUENCE   617 AA;  68806 MW;  69B7FCF6226D38A6 CRC64;
     MDLKTAVFNA ARDGKLRLLS KLLENKAKDD VVLLMSEKTN GATPLLMAAR YGHLDMVDYL
     LDQCSASVEI GGSVNFDGET IEGAPPLWAA SAAGHLKVVR SLLVHGASVN NTTLTNSTPL
     RAACFDGHLE IVKYLVEHKA DLEVANRHGH TCLMISCYKG HKEIAQFLLE KGADVNRKSV
     KGNTALHDCA ESGSLEIMQM LLKYGARMEK DGYGMTPLLS ASVTGHTNIV DFLTQNPQTS
     KNERINALEL LGATFVDKKR DLLGALKYWK RAMDMRHSDR TNIVSKPEPQ TLIMAYDYAR
     EVNTAEELDN LIADPDEMRM QALLIRERIL GPSHPDTSYY IRYRGAVYAD SGNFKRCINL
     WKYALDMQQN NLDPLSPMTA SSLLSFAELF SFMLQDRAKG LLGTTVTFDD LMGILCKSVM
     EIDRAVKQTA PPPDQVQLNK ALSIILHLIC LLEKVPCSPD QDHFKKQNIY RFLKLHPKGK
     NNFSPLHLAV DKNTTCVGRY PVCKFPSFQV TAILLECGAD VNVRDAEQNS PLHVAALNNH
     PDIMNLLVKS GAHFDSTNSH NQTACDLLDE KEMAKNLIQP INHTTLQCLA ARVIVKHNIQ
     YKQEIPEKLE SFVLLHR