ID   FEM3_CAEBR              Reviewed;         409 AA.
AC   Q8I8U6; A8Y0I5;
DT   27-SEP-2004, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2004, sequence version 2.
DT   03-AUG-2022, entry version 69.
DE   RecName: Full=Sex-determination protein fem-3;
DE   AltName: Full=Cb-FEM-3;
GN   Name=fem-3 {ECO:0000312|EMBL:AAN37405.1}; ORFNames=CBG21774;
OS   Caenorhabditis briggsae.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6238;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:AAN37405.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND INTERACTION WITH TRA-2.
RC   STRAIN=AF16 {ECO:0000312|EMBL:AAN37405.1};
RX   PubMed=12477393; DOI=10.1016/s0960-9822(02)01333-7;
RA   Haag E.S., Wang S., Kimble J.;
RT   "Rapid coevolution of the nematode sex-determining genes fem-3 and tra-2.";
RL   Curr. Biol. 12:2035-2041(2002).
RN   [2] {ECO:0000305}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AF16;
RX   PubMed=14624247; DOI=10.1371/journal.pbio.0000045;
RA   Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N.,
RA   Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P.,
RA   Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W.,
RA   Hillier L.W., Kamath R., Kuwabara P.E., Mardis E.R., Marra M.A.,
RA   Miner T.L., Minx P., Mullikin J.C., Plumb R.W., Rogers J., Schein J.E.,
RA   Sohrmann M., Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K.,
RA   Durbin R.M., Waterston R.H.;
RT   "The genome sequence of Caenorhabditis briggsae: a platform for comparative
RT   genomics.";
RL   PLoS Biol. 1:166-192(2003).
CC   -!- FUNCTION: Required for male development. In XO (male) animals, fem-3
CC       directs male differentiation in all tissues. In XX (hermaphrodite
CC       animals), it specifies the first 80 or so germ cells to be sperm.
CC       Negatively regulates male development when bound to tra-2.
CC       {ECO:0000269|PubMed:12477393}.
CC   -!- SUBUNIT: Component of a complex containing fem-1, fem-2 and fem-3 (By
CC       similarity). Interacts with fem-1 and fem-2 (via N-terminus) (By
CC       similarity). Part of a E3 ubiquitin-protein ligase complex, at least
CC       composed of cul-2, elc-1, tra-1, fem-1, fem-2 and fem-3; mediates the
CC       ubiquitination and subsequent proteasomal degradation of tra-1 (By
CC       similarity). Interacts with sel-10 (By similarity). Interacts with tra-
CC       2 (PubMed:12477393). {ECO:0000250|UniProtKB:P34691,
CC       ECO:0000269|PubMed:12477393}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AY143174; AAN37405.1; -; mRNA.
DR   EMBL; HE600966; CAP38403.1; -; Genomic_DNA.
DR   RefSeq; XP_002633007.1; XM_002632961.1.
DR   AlphaFoldDB; Q8I8U6; -.
DR   IntAct; Q8I8U6; 1.
DR   STRING; 6238.CBG21774; -.
DR   EnsemblMetazoa; CBG21774.1; CBG21774.1; WBGene00000329.
DR   GeneID; 8575004; -.
DR   KEGG; cbr:CBG_21774; -.
DR   CTD; 8575004; -.
DR   WormBase; CBG21774; CBP11911; WBGene00000329; Cbr-fem-3.
DR   eggNOG; ENOG502TI3W; Eukaryota.
DR   HOGENOM; CLU_636523_0_0_1; -.
DR   InParanoid; Q8I8U6; -.
DR   OMA; FVFQRTM; -.
DR   OrthoDB; 1759382at2759; -.
DR   Proteomes; UP000008549; Chromosome IV.
DR   GO; GO:0031462; C:Cul2-RING ubiquitin ligase complex; IEA:EnsemblMetazoa.
DR   GO; GO:0005737; C:cytoplasm; IEA:EnsemblMetazoa.
DR   GO; GO:0008287; C:protein serine/threonine phosphatase complex; IEA:EnsemblMetazoa.
DR   GO; GO:0019903; F:protein phosphatase binding; IEA:EnsemblMetazoa.
DR   GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR   GO; GO:0019102; P:male somatic sex determination; IMP:UniProtKB.
DR   GO; GO:0042006; P:masculinization of hermaphroditic germ-line; IEA:EnsemblMetazoa.
DR   GO; GO:0010628; P:positive regulation of gene expression; IEA:EnsemblMetazoa.
DR   GO; GO:0043406; P:positive regulation of MAP kinase activity; IEA:EnsemblMetazoa.
DR   GO; GO:1904146; P:positive regulation of meiotic cell cycle process involved in oocyte maturation; IEA:EnsemblMetazoa.
DR   GO; GO:0060282; P:positive regulation of oocyte development; IEA:EnsemblMetazoa.
DR   GO; GO:0071168; P:protein localization to chromatin; IEA:EnsemblMetazoa.
DR   GO; GO:1905936; P:regulation of germ cell proliferation; IEA:EnsemblMetazoa.
DR   GO; GO:0007548; P:sex differentiation; IEA:UniProtKB-KW.
DR   GO; GO:0007283; P:spermatogenesis; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Developmental protein; Differentiation; Reference proteome;
KW   Sexual differentiation; Spermatogenesis.
FT   CHAIN           1..409
FT                   /note="Sex-determination protein fem-3"
FT                   /id="PRO_0000087219"
FT   CONFLICT        33
FT                   /note="K -> R (in Ref. 1; AAN37405)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   409 AA;  48963 MW;  89AC7A13E4B7A8B2 CRC64;
     MVPDDVEPME VDDDGALIVD LNETVEEDEE TKKEKKRRKR FREKLKRFDH YSQFSGISIA
     QIDWPLIQGR SLQRSPLTGQ SFNADENIFR IDEWPRETFL QITSTLTFCA GAALLSNEKI
     TLFVFQRTMK TLVAYCNFMY HRAITHNRRQ INRIDVHELI SRNPLRFHMF LQKFLPHPDI
     NRTHFNNEFL YYFHNLYFQD ETCRLLYHDV ARYSPIINQQ GTRMSLQHQI YYPDVMRNPA
     FDALWFTSFI NPSGYSFSRF HAYRFHEALG MPPLESELII VLDWLAKLII CDIGYKVLAW
     RDARGFQGLP DLLSFQMAML EEGDPLFDLD IDYTAPPTRL FSEPTRFQTY PKFQPRRRID
     FPSRFDGFYK KRRLERGLEE IQESFIMNHF PTKPLRTVYV YTHPEERRR