ID   FEM3_CAERE              Reviewed;         439 AA.
AC   Q8I8W7;
DT   11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   25-MAY-2022, entry version 47.
DE   RecName: Full=Sex-determination protein fem-3;
DE   AltName: Full=Cr-FEM-3;
GN   Name=fem-3 {ECO:0000312|EMBL:AAN28011.1};
OS   Caenorhabditis remanei (Caenorhabditis vulgaris).
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=31234;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:AAN28011.1}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND INTERACTION WITH TRA-2.
RC   STRAIN=SB146 {ECO:0000312|EMBL:AAN28011.1};
RX   PubMed=12477393; DOI=10.1016/s0960-9822(02)01333-7;
RA   Haag E.S., Wang S., Kimble J.;
RT   "Rapid coevolution of the nematode sex-determining genes fem-3 and tra-2.";
RL   Curr. Biol. 12:2035-2041(2002).
CC   -!- FUNCTION: Required for male development. In XO (male) animals, fem-3
CC       directs male differentiation in all tissues. In XX (hermaphrodite)
CC       animals, it specifies the first 80 or so germ cells to be sperm.
CC       Negatively regulates male development when bound to tra-2. Together
CC       with fem-2 associates with the CBC(fem-1) E3 ubiquitin-protein ligase
CC       complex which mediates the ubiquitination and subsequent proteasomal
CC       degradation of tra-1 (By similarity). {ECO:0000250,
CC       ECO:0000269|PubMed:12477393}.
CC   -!- SUBUNIT: Component of a complex containing fem-1, fem-2 and fem-3.
CC       Interacts with fem-1 and fem-2 (via N-terminus). Part of a E3
CC       ubiquitin-protein ligase complex, at least composed of cul-2, elc-1,
CC       tra-1, fem-1, fem-2 and fem-3; mediates the ubiquitination and
CC       subsequent proteasomal degradation of tra-1. Interacts with tra-1.
CC       Interacts with sel-10. Interacts with tra-2.
CC       {ECO:0000250|UniProtKB:P34691}.
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DR   EMBL; AY142113; AAN28011.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q8I8W7; -.
DR   IntAct; Q8I8W7; 1.
DR   STRING; 31234.CRE14070; -.
DR   PRIDE; Q8I8W7; -.
DR   eggNOG; ENOG502TI3W; Eukaryota.
DR   HOGENOM; CLU_615752_0_0_1; -.
DR   GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR   GO; GO:0019102; P:male somatic sex determination; IMP:UniProtKB.
DR   GO; GO:0007548; P:sex differentiation; IEA:UniProtKB-KW.
DR   GO; GO:0007283; P:spermatogenesis; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Developmental protein; Differentiation; Sexual differentiation;
KW   Spermatogenesis; Ubl conjugation pathway.
FT   CHAIN           1..439
FT                   /note="Sex-determination protein fem-3"
FT                   /id="PRO_0000087221"
FT   REGION          21..45
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   439 AA;  52125 MW;  9DADE0EFC0E186C7 CRC64;
     MTCDDFRAED EQPIEVDERR RRLKRKANDD DDDDETVRER VDDAESSMEV FEAEYPPILD
     PLQDQREAKY FRERMQRFDL YSRTTGLSVD DIDWPLIRGR SLQKGRVAGI SFVYDDTRYP
     INRFSDTWLL CVTKQKLFSF GAGCVEDLNI TSFVLRRTMK VLSTYCNWLF EAAKRNNRRH
     ITHKEIQELI NRDGFRFHQY LQKFLIGRGM EYTEYNNRFF KYLHEEYNKN PGGLETIYSN
     QDFIAKETAQ ANYIYDTVRA KYGGFEELPL FRHALKISFT QPGEHYFSRF YAKRFHEALG
     CPPLDSEIIM ILDWFGVLIM NQIAYKTIRW HEEEYNDGSF PVLDSYHKAL ADESKCPKAC
     LISISLFPDD PLFDLDIDYG TNPPPVNTAY QKVRRTPRDP TPFYRLMEFE DYKSCLVKMH
     FNFSELTGEW LRKICARGR