FEMX_STAAM
ID FEMX_STAAM Reviewed; 421 AA.
AC Q7A2M4;
DT 16-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=Lipid II:glycine glycyltransferase;
DE EC=2.3.2.16;
DE AltName: Full=Factor essential for expression of methicillin resistance X;
GN Name=femX; Synonyms=fmhB; OrderedLocusNames=SAV2262;
OS Staphylococcus aureus (strain Mu50 / ATCC 700699).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=158878;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Mu50 / ATCC 700699;
RX PubMed=11418146; DOI=10.1016/s0140-6736(00)04403-2;
RA Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L.,
RA Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M.,
RA Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y.,
RA Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H.,
RA Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K.,
RA Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H.,
RA Hiramatsu K.;
RT "Whole genome sequencing of meticillin-resistant Staphylococcus aureus.";
RL Lancet 357:1225-1240(2001).
RN [2]
RP FUNCTION.
RX PubMed=15280360; DOI=10.1074/jbc.m407149200;
RA Arbeloa A., Hugonnet J.-E., Sentilhes A.-C., Josseaume N., Dubost L.,
RA Monsempes C., Blanot D., Brouard J.-P., Arthur M.;
RT "Synthesis of mosaic peptidoglycan cross-bridges by hybrid peptidoglycan
RT assembly pathways in Gram-positive bacteria.";
RL J. Biol. Chem. 279:41546-41556(2004).
CC -!- FUNCTION: Catalyzes the incorporation of the first glycine of the
CC pentaglycine interpeptide bridge, which is characteristic of the
CC S.aureus peptidoglycan. This glycine is added to the epsilon-amino
CC group of the L-lysine of the membrane-bound lipid II intermediate
CC (GlcNAc-(beta-1,4)-N-acetylmuramic acid(-L-Ala-D-iGln-L-Lys-D-Ala-D-
CC Ala)-pyrophosphoryl-undecaprenol), using glycyl-tRNA(Gly) as donor, in
CC a ribosome-independent mechanism. Involved in methicillin resistance.
CC {ECO:0000269|PubMed:15280360}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-D-isoglutaminyl-L-Lys-D-
CC Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + glycyl-
CC tRNA(Gly) = beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-D-isoglutaminyl-L-
CC Lys-(N(6)-Gly)-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl
CC diphosphate + H(+) + tRNA(Gly); Xref=Rhea:RHEA:30435, Rhea:RHEA-
CC COMP:9664, Rhea:RHEA-COMP:9683, ChEBI:CHEBI:15378, ChEBI:CHEBI:62233,
CC ChEBI:CHEBI:62234, ChEBI:CHEBI:78442, ChEBI:CHEBI:78522; EC=2.3.2.16;
CC -!- SUBUNIT: Monomer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the FemABX family. {ECO:0000305}.
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DR EMBL; BA000017; BAB58424.1; -; Genomic_DNA.
DR RefSeq; WP_000413862.1; NC_002758.2.
DR AlphaFoldDB; Q7A2M4; -.
DR SMR; Q7A2M4; -.
DR World-2DPAGE; 0002:Q7A2M4; -.
DR PaxDb; Q7A2M4; -.
DR DNASU; 1122287; -.
DR EnsemblBacteria; BAB58424; BAB58424; SAV2262.
DR KEGG; sav:SAV2262; -.
DR HOGENOM; CLU_048411_0_1_9; -.
DR OMA; FGLIQFK; -.
DR PhylomeDB; Q7A2M4; -.
DR BioCyc; MetaCyc:MON-15452; -.
DR BioCyc; SAUR158878:SAV_RS12340-MON; -.
DR Proteomes; UP000002481; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016755; F:aminoacyltransferase activity; IEA:InterPro.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0009252; P:peptidoglycan biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR InterPro; IPR016181; Acyl_CoA_acyltransferase.
DR InterPro; IPR003447; FEMABX.
DR Pfam; PF02388; FemAB; 1.
DR SUPFAM; SSF55729; SSF55729; 2.
DR PROSITE; PS51191; FEMABX; 1.
PE 3: Inferred from homology;
KW Acyltransferase; Antibiotic resistance; Cell shape;
KW Cell wall biogenesis/degradation; Cytoplasm; Peptidoglycan synthesis;
KW Transferase.
FT CHAIN 1..421
FT /note="Lipid II:glycine glycyltransferase"
FT /id="PRO_0000236171"
SQ SEQUENCE 421 AA; 48536 MW; 7250F8E6361DC6DA CRC64;
MEKMHITNQE HDAFVKSHPN GDLLQLTKWA ETKKLTGWYA RRIAVGRDGE VQGVAQLLFK
KVPKLPYTLC YISRGFVVDY SNKEALNALL DSAKEIAKAE KAYAIKIDPD VEVDKGTDAL
QNLKALGFKH KGFKEGLSKD YIQPRMTMIT PIDKNDDELL NSFERRNRSK VRLALKRGTT
VERSDREGLK TFAELMKITG ERDGFLTRDI SYFENIYDAL HEDGDAELFL VKLDPKENIA
KVNQELNELH AEIAKWQQKM ETSEKQAKKA QNMINDAQNK IAKNEDLKRD LEALEKEHPE
GIYLSGALLM FAGSKSYYLY GASSNEFRDF LPNHHMQYTM MKYAREHGAT TYDFGGTDND
PDKDSEHYGL WAFKKVWGTY LSEKIGEFDY ILNQPLYQLI EQVKPRLTKA KIKISRKLKR
K