FENR_BACCR
ID FENR_BACCR Reviewed; 331 AA.
AC Q816D9;
DT 03-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Ferredoxin--NADP reductase {ECO:0000255|HAMAP-Rule:MF_01685};
DE Short=FNR {ECO:0000255|HAMAP-Rule:MF_01685};
DE Short=Fd-NADP(+) reductase {ECO:0000255|HAMAP-Rule:MF_01685};
DE EC=1.18.1.2 {ECO:0000255|HAMAP-Rule:MF_01685};
GN OrderedLocusNames=BC_4926;
OS Bacillus cereus (strain ATCC 14579 / DSM 31 / CCUG 7414 / JCM 2152 / NBRC
OS 15305 / NCIMB 9373 / NCTC 2599 / NRRL B-3711).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC Bacillus cereus group.
OX NCBI_TaxID=226900;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 14579 / DSM 31 / CCUG 7414 / JCM 2152 / NBRC 15305 / NCIMB 9373
RC / NCTC 2599 / NRRL B-3711;
RX PubMed=12721630; DOI=10.1038/nature01582;
RA Ivanova N., Sorokin A., Anderson I., Galleron N., Candelon B., Kapatral V.,
RA Bhattacharyya A., Reznik G., Mikhailova N., Lapidus A., Chu L., Mazur M.,
RA Goltsman E., Larsen N., D'Souza M., Walunas T., Grechkin Y., Pusch G.,
RA Haselkorn R., Fonstein M., Ehrlich S.D., Overbeek R., Kyrpides N.C.;
RT "Genome sequence of Bacillus cereus and comparative analysis with Bacillus
RT anthracis.";
RL Nature 423:87-91(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H(+) + NADP(+) + 2 reduced [2Fe-2S]-[ferredoxin] = NADPH + 2
CC oxidized [2Fe-2S]-[ferredoxin]; Xref=Rhea:RHEA:20125, Rhea:RHEA-
CC COMP:10000, Rhea:RHEA-COMP:10001, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:57783,
CC ChEBI:CHEBI:58349; EC=1.18.1.2; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_01685};
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01685};
CC Note=Binds 1 FAD per subunit. {ECO:0000255|HAMAP-Rule:MF_01685};
CC -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01685}.
CC -!- SIMILARITY: Belongs to the ferredoxin--NADP reductase type 2 family.
CC {ECO:0000255|HAMAP-Rule:MF_01685}.
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DR EMBL; AE016877; AAP11799.1; -; Genomic_DNA.
DR RefSeq; NP_834598.1; NC_004722.1.
DR PDB; 6GAS; X-ray; 2.40 A; A/B/C/D=1-331.
DR PDBsum; 6GAS; -.
DR AlphaFoldDB; Q816D9; -.
DR SMR; Q816D9; -.
DR STRING; 226900.BC_4926; -.
DR EnsemblBacteria; AAP11799; AAP11799; BC_4926.
DR KEGG; bce:BC4926; -.
DR PATRIC; fig|226900.8.peg.5078; -.
DR HOGENOM; CLU_031864_5_5_9; -.
DR OMA; PEKVIYD; -.
DR Proteomes; UP000001417; Chromosome.
DR GO; GO:0004324; F:ferredoxin-NADP+ reductase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:UniProtKB-UniRule.
DR GO; GO:0050661; F:NADP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004791; F:thioredoxin-disulfide reductase activity; IBA:GO_Central.
DR GO; GO:0045454; P:cell redox homeostasis; IBA:GO_Central.
DR Gene3D; 3.50.50.60; -; 2.
DR HAMAP; MF_01685; FENR2; 1.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR023753; FAD/NAD-binding_dom.
DR InterPro; IPR022890; Fd--NADP_Rdtase_type_2.
DR Pfam; PF07992; Pyr_redox_2; 1.
DR SUPFAM; SSF51905; SSF51905; 1.
PE 1: Evidence at protein level;
KW 3D-structure; FAD; Flavoprotein; NADP; Oxidoreductase; Reference proteome.
FT CHAIN 1..331
FT /note="Ferredoxin--NADP reductase"
FT /id="PRO_0000364790"
FT BINDING 20
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01685"
FT BINDING 39
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01685"
FT BINDING 47
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01685"
FT BINDING 52
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01685"
FT BINDING 92
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01685"
FT BINDING 126
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01685"
FT BINDING 287
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01685"
FT BINDING 328
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01685"
FT STRAND 9..15
FT /evidence="ECO:0007829|PDB:6GAS"
FT HELIX 19..30
FT /evidence="ECO:0007829|PDB:6GAS"
FT STRAND 35..38
FT /evidence="ECO:0007829|PDB:6GAS"
FT STRAND 40..44
FT /evidence="ECO:0007829|PDB:6GAS"
FT HELIX 46..51
FT /evidence="ECO:0007829|PDB:6GAS"
FT STRAND 55..57
FT /evidence="ECO:0007829|PDB:6GAS"
FT STRAND 64..67
FT /evidence="ECO:0007829|PDB:6GAS"
FT HELIX 68..79
FT /evidence="ECO:0007829|PDB:6GAS"
FT HELIX 80..82
FT /evidence="ECO:0007829|PDB:6GAS"
FT STRAND 85..87
FT /evidence="ECO:0007829|PDB:6GAS"
FT STRAND 94..97
FT /evidence="ECO:0007829|PDB:6GAS"
FT STRAND 103..106
FT /evidence="ECO:0007829|PDB:6GAS"
FT STRAND 111..119
FT /evidence="ECO:0007829|PDB:6GAS"
FT STRAND 124..128
FT /evidence="ECO:0007829|PDB:6GAS"
FT HELIX 136..139
FT /evidence="ECO:0007829|PDB:6GAS"
FT TURN 140..143
FT /evidence="ECO:0007829|PDB:6GAS"
FT STRAND 144..146
FT /evidence="ECO:0007829|PDB:6GAS"
FT HELIX 151..154
FT /evidence="ECO:0007829|PDB:6GAS"
FT STRAND 158..162
FT /evidence="ECO:0007829|PDB:6GAS"
FT HELIX 166..175
FT /evidence="ECO:0007829|PDB:6GAS"
FT TURN 176..178
FT /evidence="ECO:0007829|PDB:6GAS"
FT STRAND 179..185
FT /evidence="ECO:0007829|PDB:6GAS"
FT STRAND 187..190
FT /evidence="ECO:0007829|PDB:6GAS"
FT HELIX 195..202
FT /evidence="ECO:0007829|PDB:6GAS"
FT TURN 203..205
FT /evidence="ECO:0007829|PDB:6GAS"
FT STRAND 207..209
FT /evidence="ECO:0007829|PDB:6GAS"
FT STRAND 212..218
FT /evidence="ECO:0007829|PDB:6GAS"
FT STRAND 220..222
FT /evidence="ECO:0007829|PDB:6GAS"
FT STRAND 225..230
FT /evidence="ECO:0007829|PDB:6GAS"
FT TURN 231..233
FT /evidence="ECO:0007829|PDB:6GAS"
FT STRAND 236..240
FT /evidence="ECO:0007829|PDB:6GAS"
FT STRAND 242..246
FT /evidence="ECO:0007829|PDB:6GAS"
FT STRAND 250..253
FT /evidence="ECO:0007829|PDB:6GAS"
FT HELIX 255..259
FT /evidence="ECO:0007829|PDB:6GAS"
FT STRAND 268..270
FT /evidence="ECO:0007829|PDB:6GAS"
FT STRAND 282..284
FT /evidence="ECO:0007829|PDB:6GAS"
FT HELIX 298..316
FT /evidence="ECO:0007829|PDB:6GAS"
FT HELIX 327..329
FT /evidence="ECO:0007829|PDB:6GAS"
SQ SEQUENCE 331 AA; 36748 MW; 3D910538F9E38649 CRC64;
MKVAENQKVY DITIIGGGPT GLFTAFYGGM RQASVKIIES LPQLGGQLSA LYPEKYIYDV
AGFPKVRAQE LVDNLKEQMK KFDPTVCLEE AVDTLEKQAD GIFKLVTNKQ THYSKSVIIT
AGNGAFQPRR LELEGTAKYE KKNLHYFVDD MNKFAGKRVV VFGGGDSAVD WTMMLEPIAE
KVTIVHRRDK FRAHEHSVEN LMNSRAEVST PYVPVELIGD DKIEQVVLQH VKTEEKVIID
VDDVIVNYGF VSSLGPIKNW GLDIQKNSIL VNSKMETNIP GIYAAGDICT YEGKVKLIAC
GFGEAPTAVN NAKAYFDPNA KLQPMHSSSM F
